About: Identification of Methanogenic archaea in the Hyporheic Sediment of Sitka Stream

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About: Identification of Methanogenic archaea in the Hyporheic Sediment of Sitka Stream Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Methanogenic archaea produce methane as a metabolic product under anoxic conditions and they play a crucial role in the global methane cycle. In this study molecular diversity of methanogenic archaea in the hyporheic sediment of the lowland stream Sitka (Olomouc, Czech Republic) was analyzed by PCR amplification, cloning and sequencing analysis of the methyl coenzyme M reductase alpha subunit (mcrA) gene. Sequencing analysis of 60 clones revealed 24 different mcrA phylotypes from hyporheic sedimentary layers to a depth of 50 cm. Phylotypes were affiliated with Methanomicrobiales, Methanosarcinales and Methanobacteriales orders. Only one phylotype remains unclassified. The majority of the phylotypes showed higher affiliation with uncultured methanogens than with known methanogenic species. The presence of relatively rich assemblage of methanogenic archaea confirmed that methanogens may be an important component of hyporheic microbial communities and may affect CH4 cycling in rivers. Methanogenic archaea produce methane as a metabolic product under anoxic conditions and they play a crucial role in the global methane cycle. In this study molecular diversity of methanogenic archaea in the hyporheic sediment of the lowland stream Sitka (Olomouc, Czech Republic) was analyzed by PCR amplification, cloning and sequencing analysis of the methyl coenzyme M reductase alpha subunit (mcrA) gene. Sequencing analysis of 60 clones revealed 24 different mcrA phylotypes from hyporheic sedimentary layers to a depth of 50 cm. Phylotypes were affiliated with Methanomicrobiales, Methanosarcinales and Methanobacteriales orders. Only one phylotype remains unclassified. The majority of the phylotypes showed higher affiliation with uncultured methanogens than with known methanogenic species. The presence of relatively rich assemblage of methanogenic archaea confirmed that methanogens may be an important component of hyporheic microbial communities and may affect CH4 cycling in rivers. (en)
Title	Identification of Methanogenic archaea in the Hyporheic Sediment of Sitka Stream Identification of Methanogenic archaea in the Hyporheic Sediment of Sitka Stream (en)
skos:prefLabel	Identification of Methanogenic archaea in the Hyporheic Sediment of Sitka Stream Identification of Methanogenic archaea in the Hyporheic Sediment of Sitka Stream (en)
skos:notation	RIV/61989592:15310/13:33145014!RIV14-GA0-15310___
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(GA526/09/1639)
http://linked.open...iv/cisloPeriodika	11
http://linked.open...vai/riv/dodaniDat	2014
http://linked.open...aciTvurceVysledku	Dvořák, Petr Rulík, Martin Brablcová, Lenka Buriánková, Iva Mach, Václav Chaudhary, Prem Prashant
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Palackého v Olomouci / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	78688
http://linked.open...ai/riv/idVysledku	RIV/61989592:15310/13:33145014
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	methanogenic archaea, methane, cloning, mcrA gene, hyporheic sediment (en)
http://linked.open.../riv/klicoveSlovo	hyporheic sediment methanogenic archaea mcrA gene methane cloning
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[9BAE6FFEE096]
http://linked.open...i/riv/nazevZdroje	PLoS One
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	6 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...vavai/riv/projekt	Linking biogeochemisty of methane and molecular detection of methanogens and methanotrophs in river sediments.
http://linked.open...UplatneniVysledku	2013
http://linked.open...v/svazekPeriodika	8
http://linked.open...iv/tvurceVysledku	Dvořák, Petr Rulík, Martin Mach, Václav Brablcová, Lenka Buriánková, Iva Chaudhary, Prem Prashant
http://linked.open...ain/vavai/riv/wos	000327313100113
issn	1932-6203
number of pages	10 (xsd:int)
http://bibframe.org/vocab/doi	10.1371/journal.pone.0080804
http://localhost/t...ganizacniJednotka	15310

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