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  • Capillary electrophoresis (CE) represents a high-efficient separation technique which can be used for separation of various types of compounds ranging from low-molecular weight inorganic and organic ions, to structurally big compounds as proteins, nucleic acids or objects as viruses, cells or nanoparticles. However, CE with a conventional UV detector used to suffer from a lack of detection sensitivity since the optical path of the detector is too short. This problem mostly appears if there is a need for separation of compounds with a biological or medicinal impact (toxicologically important compounds, markers of diseases, metabolites etc.). Fortunately, there is a possibility to overcome this problem by using off-line or on-line preconcentration techniques. The off-line preconcentration techniques (L-L extraction, SPE) are mostly used since they are well-known from other separations as GC or LC. On the other hand, the on-line preconcentration techniques are very interesting because the preconcentration step is immersed into the separation step. Till now, many ways how to on-line concentrate samples have been developed. A short view on following on-line preconcentration technique: sample stacking, large volume sample stacking, isotachophoresis, transient isotachophoresis, isotachophoresis in column coupling arrangement, sweeping, high-salt sample stacking, dynamic pH junction, dynamic pH junction - sweeping, field-amplified sample injection, cation/anion selective exhaustive injection - sweeping, electrokinetic supercharging, and micellar collapse will be given in this chapter. Moreover, some advances will be mentioned regarding the use of on-line preconcentration techniques on microfluidic devices.
  • Capillary electrophoresis (CE) represents a high-efficient separation technique which can be used for separation of various types of compounds ranging from low-molecular weight inorganic and organic ions, to structurally big compounds as proteins, nucleic acids or objects as viruses, cells or nanoparticles. However, CE with a conventional UV detector used to suffer from a lack of detection sensitivity since the optical path of the detector is too short. This problem mostly appears if there is a need for separation of compounds with a biological or medicinal impact (toxicologically important compounds, markers of diseases, metabolites etc.). Fortunately, there is a possibility to overcome this problem by using off-line or on-line preconcentration techniques. The off-line preconcentration techniques (L-L extraction, SPE) are mostly used since they are well-known from other separations as GC or LC. On the other hand, the on-line preconcentration techniques are very interesting because the preconcentration step is immersed into the separation step. Till now, many ways how to on-line concentrate samples have been developed. A short view on following on-line preconcentration technique: sample stacking, large volume sample stacking, isotachophoresis, transient isotachophoresis, isotachophoresis in column coupling arrangement, sweeping, high-salt sample stacking, dynamic pH junction, dynamic pH junction - sweeping, field-amplified sample injection, cation/anion selective exhaustive injection - sweeping, electrokinetic supercharging, and micellar collapse will be given in this chapter. Moreover, some advances will be mentioned regarding the use of on-line preconcentration techniques on microfluidic devices. (en)
Title
  • On-line Preconcentration Techniques in Capillary Electrophoresis
  • On-line Preconcentration Techniques in Capillary Electrophoresis (en)
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  • On-line Preconcentration Techniques in Capillary Electrophoresis
  • On-line Preconcentration Techniques in Capillary Electrophoresis (en)
skos:notation
  • RIV/61989592:15310/12:33147455!RIV14-GA0-15310___
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  • P(ED2.1.00/03.0058), P(EE2.3.20.0018), P(GAP206/12/1150)
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  • 156569
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  • RIV/61989592:15310/12:33147455
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  • Capillary Electrophoresis; On-line preconcentration (en)
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  • [654A697C75E5]
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  • New York
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  • Neuveden
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  • Capillary Electrophoresis: Fundamentals, Techniques and Applications
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  • Petr, Jan
  • Maier, Vítězslav
  • Ševčík, Juraj
number of pages
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  • Nova Science Publishers, Inc.
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  • 978-1-62081-785-8
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  • 15310
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