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Description
| - Capillary electrophoresis (CE) represents a high-efficient separation technique which can be used for separation of various types of compounds ranging from low-molecular weight inorganic and organic ions, to structurally big compounds as proteins, nucleic acids or objects as viruses, cells or nanoparticles. However, CE with a conventional UV detector used to suffer from a lack of detection sensitivity since the optical path of the detector is too short. This problem mostly appears if there is a need for separation of compounds with a biological or medicinal impact (toxicologically important compounds, markers of diseases, metabolites etc.). Fortunately, there is a possibility to overcome this problem by using off-line or on-line preconcentration techniques. The off-line preconcentration techniques (L-L extraction, SPE) are mostly used since they are well-known from other separations as GC or LC. On the other hand, the on-line preconcentration techniques are very interesting because the preconcentration step is immersed into the separation step. Till now, many ways how to on-line concentrate samples have been developed. A short view on following on-line preconcentration technique: sample stacking, large volume sample stacking, isotachophoresis, transient isotachophoresis, isotachophoresis in column coupling arrangement, sweeping, high-salt sample stacking, dynamic pH junction, dynamic pH junction - sweeping, field-amplified sample injection, cation/anion selective exhaustive injection - sweeping, electrokinetic supercharging, and micellar collapse will be given in this chapter. Moreover, some advances will be mentioned regarding the use of on-line preconcentration techniques on microfluidic devices.
- Capillary electrophoresis (CE) represents a high-efficient separation technique which can be used for separation of various types of compounds ranging from low-molecular weight inorganic and organic ions, to structurally big compounds as proteins, nucleic acids or objects as viruses, cells or nanoparticles. However, CE with a conventional UV detector used to suffer from a lack of detection sensitivity since the optical path of the detector is too short. This problem mostly appears if there is a need for separation of compounds with a biological or medicinal impact (toxicologically important compounds, markers of diseases, metabolites etc.). Fortunately, there is a possibility to overcome this problem by using off-line or on-line preconcentration techniques. The off-line preconcentration techniques (L-L extraction, SPE) are mostly used since they are well-known from other separations as GC or LC. On the other hand, the on-line preconcentration techniques are very interesting because the preconcentration step is immersed into the separation step. Till now, many ways how to on-line concentrate samples have been developed. A short view on following on-line preconcentration technique: sample stacking, large volume sample stacking, isotachophoresis, transient isotachophoresis, isotachophoresis in column coupling arrangement, sweeping, high-salt sample stacking, dynamic pH junction, dynamic pH junction - sweeping, field-amplified sample injection, cation/anion selective exhaustive injection - sweeping, electrokinetic supercharging, and micellar collapse will be given in this chapter. Moreover, some advances will be mentioned regarding the use of on-line preconcentration techniques on microfluidic devices. (en)
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Title
| - On-line Preconcentration Techniques in Capillary Electrophoresis
- On-line Preconcentration Techniques in Capillary Electrophoresis (en)
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skos:prefLabel
| - On-line Preconcentration Techniques in Capillary Electrophoresis
- On-line Preconcentration Techniques in Capillary Electrophoresis (en)
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skos:notation
| - RIV/61989592:15310/12:33147455!RIV14-GA0-15310___
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http://linked.open...avai/riv/aktivita
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http://linked.open...avai/riv/aktivity
| - P(ED2.1.00/03.0058), P(EE2.3.20.0018), P(GAP206/12/1150)
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http://linked.open...vai/riv/dodaniDat
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http://linked.open...aciTvurceVysledku
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http://linked.open.../riv/druhVysledku
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http://linked.open...iv/duvernostUdaju
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http://linked.open...titaPredkladatele
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http://linked.open...dnocenehoVysledku
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http://linked.open...ai/riv/idVysledku
| - RIV/61989592:15310/12:33147455
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http://linked.open...riv/jazykVysledku
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http://linked.open.../riv/klicovaSlova
| - Capillary Electrophoresis; On-line preconcentration (en)
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http://linked.open.../riv/klicoveSlovo
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http://linked.open...ontrolniKodProRIV
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http://linked.open...i/riv/mistoVydani
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http://linked.open...vEdiceCisloSvazku
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http://linked.open...i/riv/nazevZdroje
| - Capillary Electrophoresis: Fundamentals, Techniques and Applications
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http://linked.open...in/vavai/riv/obor
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http://linked.open...ichTvurcuVysledku
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http://linked.open...v/pocetStranKnihy
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http://linked.open...cetTvurcuVysledku
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http://linked.open...vavai/riv/projekt
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http://linked.open...UplatneniVysledku
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http://linked.open...iv/tvurceVysledku
| - Petr, Jan
- Maier, Vítězslav
- Ševčík, Juraj
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number of pages
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http://purl.org/ne...btex#hasPublisher
| - Nova Science Publishers, Inc.
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https://schema.org/isbn
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http://localhost/t...ganizacniJednotka
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