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Description
  • Antioxidant capacity and various classes of phenolic antioxidants were quantified in leaf and bark methanol extracts of a medicinal plant, Rhamnus intermedia Steud. et Hochst., whose phytochemical composition is unknown. Three well-established assays were used to determine the antioxidant potency of the extracts: the Ferric Reducing/Antioxidant Power assay (FRAP), 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay, and 2,2'-azinobis (3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) assay, as well as oxygen radical absorbance capacity (ORAC) assay. Total phenol (TP, Folin-Ciocalteu assay), total flavonoid (IF, colorimetric assay with AlCl3) and free phenolic acid (UPLC-MS/MS) content of extracts was quantified. In comparison to bark extracts, leaf extracts exhibited significantly higher TP and IF contents, as well as higher antioxidant capacity according to all four assays. Ten free phenolic acids were quantified in leaf extracts while six were detected in bark extracts. The most abundant phenolic acid in leaves was vanilic acid (1647.06 +/- 79.35 mu mol g(-1) DW; DW, dry weight) while salycilic acid was the most concentrated in the bark (111.10 +/- 14.14 mu mol g(-1) DW). (doi: 10.5562/cca1946)
  • Antioxidant capacity and various classes of phenolic antioxidants were quantified in leaf and bark methanol extracts of a medicinal plant, Rhamnus intermedia Steud. et Hochst., whose phytochemical composition is unknown. Three well-established assays were used to determine the antioxidant potency of the extracts: the Ferric Reducing/Antioxidant Power assay (FRAP), 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay, and 2,2'-azinobis (3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) assay, as well as oxygen radical absorbance capacity (ORAC) assay. Total phenol (TP, Folin-Ciocalteu assay), total flavonoid (IF, colorimetric assay with AlCl3) and free phenolic acid (UPLC-MS/MS) content of extracts was quantified. In comparison to bark extracts, leaf extracts exhibited significantly higher TP and IF contents, as well as higher antioxidant capacity according to all four assays. Ten free phenolic acids were quantified in leaf extracts while six were detected in bark extracts. The most abundant phenolic acid in leaves was vanilic acid (1647.06 +/- 79.35 mu mol g(-1) DW; DW, dry weight) while salycilic acid was the most concentrated in the bark (111.10 +/- 14.14 mu mol g(-1) DW). (doi: 10.5562/cca1946) (en)
Title
  • Rhamnus intermedia Steud. et Hochst. - a New Source of Bioactive Phytochemicals
  • Rhamnus intermedia Steud. et Hochst. - a New Source of Bioactive Phytochemicals (en)
skos:prefLabel
  • Rhamnus intermedia Steud. et Hochst. - a New Source of Bioactive Phytochemicals
  • Rhamnus intermedia Steud. et Hochst. - a New Source of Bioactive Phytochemicals (en)
skos:notation
  • RIV/61989592:15310/12:33142889!RIV13-MSM-15310___
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(KAN200380801), Z(AV0Z50380511), Z(MSM6198959216)
http://linked.open...iv/cisloPeriodika
  • 2
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 165532
http://linked.open...ai/riv/idVysledku
  • RIV/61989592:15310/12:33142889
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • phenolic acids; total flavonoids; total phenols; antioxidant activity; Rhamnus intermedia (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • HR - Chorvatská republika
http://linked.open...ontrolniKodProRIV
  • [993E7F815905]
http://linked.open...i/riv/nazevZdroje
  • Croatica Chemica Acta
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 85
http://linked.open...iv/tvurceVysledku
  • Grúz, Jiří
  • Jurišić Grubešić, Renata
  • Kremer, Dario
  • Piljac-Žegarac, Jasenka
  • Šamec, Dunja
http://linked.open...ain/vavai/riv/wos
  • 000306880200001
http://linked.open...n/vavai/riv/zamer
issn
  • 0011-1643
number of pages
http://bibframe.org/vocab/doi
  • 10.5562/cca1946
http://localhost/t...ganizacniJednotka
  • 15310
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