About: Comparison of endogenous cytokinins and cytokinin oxidase/dehydrogenase activity in germinating and thermoinhibited Tagetes minuta achenes     Goto   Sponge   NotDistinct   Permalink

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Description
  • Tagetes minuta L achenes are thermoinhibited at temperatures above 35 degrees C and have accelerated radicle emergence (germination) when subsequently transferred to an optimal temperature (25 degrees C). Endogenous cytokinins and cytokinin oxidase/dehydrogenase (CKX) activity were compared in normally germinating (25 degrees C) and thermoinhibited (72 h at 36 degrees C then transferred to 25 degrees C) T. minuta achenes. Following imbibition, endogenous cytokinin concentrations changed in normally germinating T. minuta achenes, with a gradual decrease in dihydrozeatin-type (DHZ) cytokinins, a large increase in cis-zeatin-type (cZ) cytokinins, a smaller increase in N-6-(2-isopentenyl)adenine-type (iP) cytokinins and a peak of transzeatin-type (tZ) cytokinins at 13 h. These changes in the isoprenoid cytokinin profile were similar in the thermoinhibited achenes imbibed at 36 degrees C, despite the thermal block preventing radicle emergence. The exception was the iP-type cytokinins that only increased when transferred to 25 degrees C. Profiles of the physiologically active free bases showed an increase in tZ prior to radical emergence in both normally germinating (13 h) and thermoinhibited achenes. A large transient peak in aromatic cytokinins [N-6-benzyladenine-type (BA)] occurred during early seedling establishment in normally germinating achenes (40 h) while a transient maximum in BA-type cytokinins was found prior to radicle emergence in the thermoinhibited achenes (24 h). The CKX activity was enhanced in normally germinating achenes as the cytokinin concentration increased following imbibition. In thermoinhibited achenes, an elevated temperature negatively affected the CKX activity that only increased when the achenes were transferred to 25 degrees C, corresponding to an increase in iP-type cytokinins.
  • Tagetes minuta L achenes are thermoinhibited at temperatures above 35 degrees C and have accelerated radicle emergence (germination) when subsequently transferred to an optimal temperature (25 degrees C). Endogenous cytokinins and cytokinin oxidase/dehydrogenase (CKX) activity were compared in normally germinating (25 degrees C) and thermoinhibited (72 h at 36 degrees C then transferred to 25 degrees C) T. minuta achenes. Following imbibition, endogenous cytokinin concentrations changed in normally germinating T. minuta achenes, with a gradual decrease in dihydrozeatin-type (DHZ) cytokinins, a large increase in cis-zeatin-type (cZ) cytokinins, a smaller increase in N-6-(2-isopentenyl)adenine-type (iP) cytokinins and a peak of transzeatin-type (tZ) cytokinins at 13 h. These changes in the isoprenoid cytokinin profile were similar in the thermoinhibited achenes imbibed at 36 degrees C, despite the thermal block preventing radicle emergence. The exception was the iP-type cytokinins that only increased when transferred to 25 degrees C. Profiles of the physiologically active free bases showed an increase in tZ prior to radical emergence in both normally germinating (13 h) and thermoinhibited achenes. A large transient peak in aromatic cytokinins [N-6-benzyladenine-type (BA)] occurred during early seedling establishment in normally germinating achenes (40 h) while a transient maximum in BA-type cytokinins was found prior to radicle emergence in the thermoinhibited achenes (24 h). The CKX activity was enhanced in normally germinating achenes as the cytokinin concentration increased following imbibition. In thermoinhibited achenes, an elevated temperature negatively affected the CKX activity that only increased when the achenes were transferred to 25 degrees C, corresponding to an increase in iP-type cytokinins. (en)
Title
  • Comparison of endogenous cytokinins and cytokinin oxidase/dehydrogenase activity in germinating and thermoinhibited Tagetes minuta achenes
  • Comparison of endogenous cytokinins and cytokinin oxidase/dehydrogenase activity in germinating and thermoinhibited Tagetes minuta achenes (en)
skos:prefLabel
  • Comparison of endogenous cytokinins and cytokinin oxidase/dehydrogenase activity in germinating and thermoinhibited Tagetes minuta achenes
  • Comparison of endogenous cytokinins and cytokinin oxidase/dehydrogenase activity in germinating and thermoinhibited Tagetes minuta achenes (en)
skos:notation
  • RIV/61989592:15310/12:33142885!RIV13-MSM-15310___
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(ED0007/01/01), P(GAP506/11/0774), P(LC06034), P(TA01010861), S, Z(AV0Z50380511)
http://linked.open...iv/cisloPeriodika
  • 7
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 127912
http://linked.open...ai/riv/idVysledku
  • RIV/61989592:15310/12:33142885
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • Thermoinhibition; Germination; Deactivation; Cytokinin oxidase/dehydrogenase; Cytokinin biosynthesis (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • DE - Spolková republika Německo
http://linked.open...ontrolniKodProRIV
  • [957E84C0A001]
http://linked.open...i/riv/nazevZdroje
  • Journal of Plant Physiology
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 169
http://linked.open...iv/tvurceVysledku
  • Motyka, Václav
  • Novák, Ondřej
  • Strnad, Miroslav
  • Žižková, Eva
  • Van Staden, Johannes
  • Stirk, Wendy A.
http://linked.open...ain/vavai/riv/wos
  • 000304335300007
http://linked.open...n/vavai/riv/zamer
issn
  • 0176-1617
number of pages
http://bibframe.org/vocab/doi
  • 10.1016/j.jplph.2012.01.013
http://localhost/t...ganizacniJednotka
  • 15310
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