About: Reproducible discrimination between Gram-positive and Gram-negative bacteria using surface enhanced Raman spectroscopy with infrared excitation     Goto   Sponge   NotDistinct   Permalink

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  • The on time diagnostics of bacterial diseases is one of the essential steps in the foregoing treatment of such pathogens. Here we sought to present an easy to use and robust method for the discrimination between Gram-positive (Enterococcus faecalis and Streptococcus pyogenes) and Gram-negative (Acinetobacter baumannii and Klebsiella pneumoniae) bacterial genera based on surface enhanced Raman scattering (SERS) spectroscopy. The robustness of our approach lies in the novel method for the production of the SER substrate based on silver nanoparticles and their subsequent recrystallization in solutions containing high concentrations of chloride ions. The method presented here could be an interesting alternative both to commonly used histochemical approaches and commercial SERS substrates.
  • The on time diagnostics of bacterial diseases is one of the essential steps in the foregoing treatment of such pathogens. Here we sought to present an easy to use and robust method for the discrimination between Gram-positive (Enterococcus faecalis and Streptococcus pyogenes) and Gram-negative (Acinetobacter baumannii and Klebsiella pneumoniae) bacterial genera based on surface enhanced Raman scattering (SERS) spectroscopy. The robustness of our approach lies in the novel method for the production of the SER substrate based on silver nanoparticles and their subsequent recrystallization in solutions containing high concentrations of chloride ions. The method presented here could be an interesting alternative both to commonly used histochemical approaches and commercial SERS substrates. (en)
Title
  • Reproducible discrimination between Gram-positive and Gram-negative bacteria using surface enhanced Raman spectroscopy with infrared excitation
  • Reproducible discrimination between Gram-positive and Gram-negative bacteria using surface enhanced Raman spectroscopy with infrared excitation (en)
skos:prefLabel
  • Reproducible discrimination between Gram-positive and Gram-negative bacteria using surface enhanced Raman spectroscopy with infrared excitation
  • Reproducible discrimination between Gram-positive and Gram-negative bacteria using surface enhanced Raman spectroscopy with infrared excitation (en)
skos:notation
  • RIV/61989592:15310/12:33142310!RIV13-MSM-15310___
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(ED2.1.00/03.0058), P(EE2.3.20.0056), P(GAP304/10/1316), S
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  • 12
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  • 165072
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  • RIV/61989592:15310/12:33142310
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http://linked.open.../riv/klicovaSlova
  • Bacteria discrimination, Gram-positive, Gram-negative, surface enhanced Raman spectroscopy, silver nanoparticles (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • GB - Spojené království Velké Británie a Severního Irska
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  • [2A7375CA0DD3]
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  • Analyst
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  • 137
http://linked.open...iv/tvurceVysledku
  • Kvítek, Libor
  • Kolář, Milan
  • Panáček, Aleš
  • Prucek, Robert
  • Zbořil, Radek
  • Ranc, Václav
http://linked.open...ain/vavai/riv/wos
  • 000304308700020
issn
  • 0003-2654
number of pages
http://bibframe.org/vocab/doi
  • 10.1039/c2an16310a
http://localhost/t...ganizacniJednotka
  • 15310
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