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  • An electrochemical biosensor for detection of the plant hormone cytokinin is introduced. Cytokinin homeostasis in tissues of many lower and higher plants is controlled largely by the activity of cytokinin dehydrogenase (CKX, EC 1.5.99.12) that catalyzes an irreversible cleavage of N6-side chain of cytokinins. Expression of Arabidopsis thaliana CKX2 from Pichia pastoris was used to prepare purified AtCKX2 as the basis of the cytokinin biosensor. Prussian Blue (PrB) was electrodeposited on Pt microelectrodes prior to deposition of the enzyme in a sol-gel matrix. The biosensor gave amperometric responses to several cytokinins. These responses depended on the presence of both the enzyme and the Prussian Blue. Thus Prussian Blue must act as an electron mediator between the FAD centre in CKX2 and the Pt surface.
  • An electrochemical biosensor for detection of the plant hormone cytokinin is introduced. Cytokinin homeostasis in tissues of many lower and higher plants is controlled largely by the activity of cytokinin dehydrogenase (CKX, EC 1.5.99.12) that catalyzes an irreversible cleavage of N6-side chain of cytokinins. Expression of Arabidopsis thaliana CKX2 from Pichia pastoris was used to prepare purified AtCKX2 as the basis of the cytokinin biosensor. Prussian Blue (PrB) was electrodeposited on Pt microelectrodes prior to deposition of the enzyme in a sol-gel matrix. The biosensor gave amperometric responses to several cytokinins. These responses depended on the presence of both the enzyme and the Prussian Blue. Thus Prussian Blue must act as an electron mediator between the FAD centre in CKX2 and the Pt surface. (en)
Title
  • Prussian Blue acts as a mediator in a reagentless cytokinin biosensor.
  • Prussian Blue acts as a mediator in a reagentless cytokinin biosensor. (en)
skos:prefLabel
  • Prussian Blue acts as a mediator in a reagentless cytokinin biosensor.
  • Prussian Blue acts as a mediator in a reagentless cytokinin biosensor. (en)
skos:notation
  • RIV/61989592:15310/11:33118639!RIV12-MSM-15310___
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(ED0007/01/01)
http://linked.open...iv/cisloPeriodika
  • 701
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 225025
http://linked.open...ai/riv/idVysledku
  • RIV/61989592:15310/11:33118639
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • Electrode; Quantitation; Dehydrogenase; Oxidase; Electrochemistry; Phytohormone (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • NL - Nizozemsko
http://linked.open...ontrolniKodProRIV
  • [4FE429585964]
http://linked.open...i/riv/nazevZdroje
  • Analytica Chimica Acta
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 701
http://linked.open...iv/tvurceVysledku
  • Frébort, Ivo
  • Galuszka, Petr
  • Kowalska, Marta
  • Dale, Nicholas
  • Napier, Richard
  • Tian, Faming
  • Šmehilová, Mária
http://linked.open...ain/vavai/riv/wos
  • 000294144400015
issn
  • 0003-2670
number of pages
http://bibframe.org/vocab/doi
  • 10.1016/j.aca.2011.06.018
http://localhost/t...ganizacniJednotka
  • 15310
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