About: Quantification of DNA content in freshwater microalgae using flow cytometry: a modified protocol for selected green microalgae     Goto   Sponge   NotDistinct   Permalink

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Description
  • We propose a multi-step protocol that leads to the quantification of DNA content in desmids using flow cytometry. We present detailed culture conditions, the minimal biomass necessary for three repetitive measurements, a method to isolate protoplasts and selection of suitable standards. We suggest and characterize a new standard for microagae - Micrasterias pinnatifida.
  • We propose a multi-step protocol that leads to the quantification of DNA content in desmids using flow cytometry. We present detailed culture conditions, the minimal biomass necessary for three repetitive measurements, a method to isolate protoplasts and selection of suitable standards. We suggest and characterize a new standard for microagae - Micrasterias pinnatifida. (en)
Title
  • Quantification of DNA content in freshwater microalgae using flow cytometry: a modified protocol for selected green microalgae
  • Quantification of DNA content in freshwater microalgae using flow cytometry: a modified protocol for selected green microalgae (en)
skos:prefLabel
  • Quantification of DNA content in freshwater microalgae using flow cytometry: a modified protocol for selected green microalgae
  • Quantification of DNA content in freshwater microalgae using flow cytometry: a modified protocol for selected green microalgae (en)
skos:notation
  • RIV/61989592:15310/11:10223907!RIV12-MSM-15310___
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(GA206/08/0389), P(GA206/09/0906), S
http://linked.open...iv/cisloPeriodika
  • 2
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 225405
http://linked.open...ai/riv/idVysledku
  • RIV/61989592:15310/11:10223907
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • DNA content, flow cytometry, desmids, microalgae, standards, Micrasterias pinnatifida (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • CZ - Česká republika
http://linked.open...ontrolniKodProRIV
  • [B097D1194F4E]
http://linked.open...i/riv/nazevZdroje
  • Fottea
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 11
http://linked.open...iv/tvurceVysledku
  • Mazalová, Petra
  • Ondřej, Vladan
  • Poulíčková, Aloisie
  • Šarhanová, Petra
http://linked.open...ain/vavai/riv/wos
  • 000296310200006
issn
  • 1802-5439
number of pages
http://localhost/t...ganizacniJednotka
  • 15310
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