About: Polyploidization Facilitates Biotechnological In Vitro Techniques in the Genus Cucumis     Goto   Sponge   NotDistinct   Permalink

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  • Prezygotic interspecific crossability barrier in the genus Cucumis is related to the ploidy level of the species (cucumber (C. sativus), x = 7; muskmelon (C. melo) and wild Cucumis species, x = 12). Polyploidization of maternal plants helps hybridization among other Cucumis species by overcoming prezygotic genetic barriers. The main objective of this paper is to compare the results of several methods supporting interspecific crosses in cucumber without and with polyploidization (comparison between diploid (2x) and mixoploid (2x/4x) cucumber maternal plants). Mixoploid plants were obtained after in vivo and in vitro polyploidization by colchicine and oryzalin. Ploidy level was estimated by flow cytometry. Embryo rescue, in vitro pollination, and isolation of mesophyll protoplast were tested and compared. Positive effect of polyploidization was observed during all experiments presented by higher regeneration capacity of cultivated mixoploid cucumber embryos, ovules, and protoplasts.
  • Prezygotic interspecific crossability barrier in the genus Cucumis is related to the ploidy level of the species (cucumber (C. sativus), x = 7; muskmelon (C. melo) and wild Cucumis species, x = 12). Polyploidization of maternal plants helps hybridization among other Cucumis species by overcoming prezygotic genetic barriers. The main objective of this paper is to compare the results of several methods supporting interspecific crosses in cucumber without and with polyploidization (comparison between diploid (2x) and mixoploid (2x/4x) cucumber maternal plants). Mixoploid plants were obtained after in vivo and in vitro polyploidization by colchicine and oryzalin. Ploidy level was estimated by flow cytometry. Embryo rescue, in vitro pollination, and isolation of mesophyll protoplast were tested and compared. Positive effect of polyploidization was observed during all experiments presented by higher regeneration capacity of cultivated mixoploid cucumber embryos, ovules, and protoplasts. (en)
Title
  • Polyploidization Facilitates Biotechnological In Vitro Techniques in the Genus Cucumis
  • Polyploidization Facilitates Biotechnological In Vitro Techniques in the Genus Cucumis (en)
skos:prefLabel
  • Polyploidization Facilitates Biotechnological In Vitro Techniques in the Genus Cucumis
  • Polyploidization Facilitates Biotechnological In Vitro Techniques in the Genus Cucumis (en)
skos:notation
  • RIV/61989592:15310/10:10212970!RIV11-MSM-15310___
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • Z(MSM6198959215)
http://linked.open...iv/cisloPeriodika
  • ID 475432
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 279808
http://linked.open...ai/riv/idVysledku
  • RIV/61989592:15310/10:10212970
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • in vitro techniques; Biotechnology; Polyploidization; Cucumis spp. (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • US - Spojené státy americké
http://linked.open...ontrolniKodProRIV
  • [B9A6BC7A702C]
http://linked.open...i/riv/nazevZdroje
  • Journal of Biomedicine and Biotechnology (online)
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 2010
http://linked.open...iv/tvurceVysledku
  • Lebeda, Aleš
  • Navrátilová, Božena
  • Ondřej, Vladan
  • Skálová, Dagmar
  • Doležalová, Ivana
http://linked.open...n/vavai/riv/zamer
issn
  • 1110-7251
number of pages
http://localhost/t...ganizacniJednotka
  • 15310
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