About: MAP65 in tubulin/colchicine paracrystals of Vigna sinensis root cells: Possible role in the assembly and stabilization of atypical tubulin polymers     Goto   Sponge   NotDistinct   Permalink

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  • Members of the MAP65 family, colocalizing with microtubule arrays, have been identified in Vigna sinensis root cells by Western blotting and immunofluorescence. MAP65 proteins were also found in tubulin/colchicine-induced paracrystals. During recovery from colchicine, MAP65 signal was depleted from disintegrating paracrystals appearing in the reinstating microtubule arrays. MAP65-free perinuclear tubulin/colchicine aggregates were observed in plasmolyzed colchicine-treated cells. Deplasmolysis of the above cells resulted in the formation of MAP65-decorated paracrystals. MAP65 proteins underwent phosphorylation during plasmolysis, which was reversible by deplasmolysis. It seems likely that apart from binding to microtubules, MAP65 proteins may act as %22tubulin associated proteins%22 in a broader manner, promoting the polymerization and/or stabilization of atypical polymers such as tubulin/colchicine paracrystals.
  • Members of the MAP65 family, colocalizing with microtubule arrays, have been identified in Vigna sinensis root cells by Western blotting and immunofluorescence. MAP65 proteins were also found in tubulin/colchicine-induced paracrystals. During recovery from colchicine, MAP65 signal was depleted from disintegrating paracrystals appearing in the reinstating microtubule arrays. MAP65-free perinuclear tubulin/colchicine aggregates were observed in plasmolyzed colchicine-treated cells. Deplasmolysis of the above cells resulted in the formation of MAP65-decorated paracrystals. MAP65 proteins underwent phosphorylation during plasmolysis, which was reversible by deplasmolysis. It seems likely that apart from binding to microtubules, MAP65 proteins may act as %22tubulin associated proteins%22 in a broader manner, promoting the polymerization and/or stabilization of atypical polymers such as tubulin/colchicine paracrystals. (en)
Title
  • MAP65 in tubulin/colchicine paracrystals of Vigna sinensis root cells: Possible role in the assembly and stabilization of atypical tubulin polymers
  • MAP65 in tubulin/colchicine paracrystals of Vigna sinensis root cells: Possible role in the assembly and stabilization of atypical tubulin polymers (en)
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  • MAP65 in tubulin/colchicine paracrystals of Vigna sinensis root cells: Possible role in the assembly and stabilization of atypical tubulin polymers
  • MAP65 in tubulin/colchicine paracrystals of Vigna sinensis root cells: Possible role in the assembly and stabilization of atypical tubulin polymers (en)
skos:notation
  • RIV/61989592:15310/10:10212486!RIV11-MSM-15310___
http://linked.open...avai/riv/aktivita
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  • P(ED0007/01/01)
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  • 3
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  • 269666
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  • RIV/61989592:15310/10:10212486
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  • phosphorylation; protein; Phos-tag; microtubules; MAP65 (en)
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  • US - Spojené státy americké
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  • [404815CD290B]
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  • Cytoskeleton (online)
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http://linked.open...v/svazekPeriodika
  • 67
http://linked.open...iv/tvurceVysledku
  • Šamaj, Jozef
  • Komis, George
  • Adamakis, Ioannis-Dimosthenis
  • Bosabalidis, Artemios
  • Panteris, Emmanuel
http://linked.open...ain/vavai/riv/wos
  • 000276458100003
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  • 1949-3592
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  • 15310
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