About: Genetic variability of stolbur phytoplasma in annual crop and wild plant species in South Moravia

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About: Genetic variability of stolbur phytoplasma in annual crop and wild plant species in South Moravia Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The genetic diversity of stolbur phytoplasma isolates was assessed by different molecular typing methods in a wide range of plant hosts in a limited geographical area of the Czech Republic. Plants of Apium graveolens, Capsicum annuum, Solanum lycopersicum, Solanum tuberosum, Amaranthus retroflexus, Calystegia sepium, Cirsium arvense, Convolvulus arvensis, and Datura stramonium displaying symptoms of proliferation, discoloration, and malformation of flower were collected in vegetable crop plots in the vicinity of the village of Lednice (south Moravia) and examined for the presence of phytoplasmas. Using 16S rDNA amplification and RFLP assays, stolbur phytoplasma was detected in some of the symptomatic plants. The genotype of the phytoplasma isolates detected was investigated by restriction pattern or sequence analysis of two non-ribosomal house-keeping genes (tuf and secY) and a recently characterized polymorphic gene (vmp1). According to tuf genotyping, all isolates were of the tufAY-b genotype sensu The genetic diversity of stolbur phytoplasma isolates was assessed by different molecular typing methods in a wide range of plant hosts in a limited geographical area of the Czech Republic. Plants of Apium graveolens, Capsicum annuum, Solanum lycopersicum, Solanum tuberosum, Amaranthus retroflexus, Calystegia sepium, Cirsium arvense, Convolvulus arvensis, and Datura stramonium displaying symptoms of proliferation, discoloration, and malformation of flower were collected in vegetable crop plots in the vicinity of the village of Lednice (south Moravia) and examined for the presence of phytoplasmas. Using 16S rDNA amplification and RFLP assays, stolbur phytoplasma was detected in some of the symptomatic plants. The genotype of the phytoplasma isolates detected was investigated by restriction pattern or sequence analysis of two non-ribosomal house-keeping genes (tuf and secY) and a recently characterized polymorphic gene (vmp1). According to tuf genotyping, all isolates were of the tufAY-b genotype sensu (en)
Title	Genetic variability of stolbur phytoplasma in annual crop and wild plant species in South Moravia Genetic variability of stolbur phytoplasma in annual crop and wild plant species in South Moravia (en)
skos:prefLabel	Genetic variability of stolbur phytoplasma in annual crop and wild plant species in South Moravia Genetic variability of stolbur phytoplasma in annual crop and wild plant species in South Moravia (en)
skos:notation	RIV/61989592:15310/09:00010866!RIV10-GA0-15310___
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(GA522/06/0618)
http://linked.open...iv/cisloPeriodika	2
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Navrátil, Milan Fialová, Renata Šafářová, Dana Válová, Pavla
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Palackého v Olomouci / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	316165
http://linked.open...ai/riv/idVysledku	RIV/61989592:15310/09:00010866
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	stolbur disease; non-ribosomal DNA; vmp1 gene; tuf gene; secY gene; PCR; RFLP SEQUENCE; CLASSIFICATION; GRAPEVINE; VECTOR; RNA; DNA (en)
http://linked.open.../riv/klicoveSlovo	GRAPEVINE RFLP SEQUENCE non-ribosomal DNA secY gene stolbur disease tuf gene vmp1 gene DNA PCR RNA CLASSIFICATION VECTOR
http://linked.open...odStatuVydavatele	IT - Italská republika
http://linked.open...ontrolniKodProRIV	[61A249A42A7B]
http://linked.open...i/riv/nazevZdroje	Journal of Plant Pathology
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	7 (xsd:int)
http://linked.open...vavai/riv/projekt	Epidemiology of stolbur disease
http://linked.open...UplatneniVysledku	2009
http://linked.open...v/svazekPeriodika	91
http://linked.open...iv/tvurceVysledku	Navrátil, Milan Šafářová, Dana Válová, Pavla Danet, Jean-Luc Foissac, Xavier Fialová, Renata Balakishiyeva, G.
issn	1125-4653
number of pages	6 (xsd:int)
http://localhost/t...ganizacniJednotka	15310

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