About: Solving the problem of shoot-tip necrosis in Harpagophytum procumbens by changing the cytokinin types, calcium and boron concentrations in the medium

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About: Solving the problem of shoot-tip necrosis in Harpagophytum procumbens by changing the cytokinin types, calcium and boron concentrations in the medium Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Various experiments were conducted to characterize and control factors affecting shoot-tip necrosis (STN) in Harpagophytum procumbens. Higher cytokinin concentrations increased the incidence of STN and the problem was aggravated by the addition of auxin (IAA) to the multiplication medium. Optimum shoot multiplication was achieved by omitting auxin and using the cytokinin meta-Topolin riboside (mTR). In the presence of auxin, plantlets produced basal callus that interfered with rooting. The quantity of this basal callus was minimal when mTR was used. Increasing the concentration of either calcium or boron prevented the development of necrotic shoots. When the concentration of both elements was increased simultaneously, negative effects on both growth and STN were observed. Using 6 mM Ca in half-strength MS medium was optimum. Boron was toxic at higher (0.4 and 0.5 mM) concentrations. Plantlets rooted readily in half-strength cytokinin-free MS media supplemented with 2.5 mu M IAA. Rooted plantlets produ Various experiments were conducted to characterize and control factors affecting shoot-tip necrosis (STN) in Harpagophytum procumbens. Higher cytokinin concentrations increased the incidence of STN and the problem was aggravated by the addition of auxin (IAA) to the multiplication medium. Optimum shoot multiplication was achieved by omitting auxin and using the cytokinin meta-Topolin riboside (mTR). In the presence of auxin, plantlets produced basal callus that interfered with rooting. The quantity of this basal callus was minimal when mTR was used. Increasing the concentration of either calcium or boron prevented the development of necrotic shoots. When the concentration of both elements was increased simultaneously, negative effects on both growth and STN were observed. Using 6 mM Ca in half-strength MS medium was optimum. Boron was toxic at higher (0.4 and 0.5 mM) concentrations. Plantlets rooted readily in half-strength cytokinin-free MS media supplemented with 2.5 mu M IAA. Rooted plantlets produ (en)
Title	Solving the problem of shoot-tip necrosis in Harpagophytum procumbens by changing the cytokinin types, calcium and boron concentrations in the medium Solving the problem of shoot-tip necrosis in Harpagophytum procumbens by changing the cytokinin types, calcium and boron concentrations in the medium (en)
skos:prefLabel	Solving the problem of shoot-tip necrosis in Harpagophytum procumbens by changing the cytokinin types, calcium and boron concentrations in the medium Solving the problem of shoot-tip necrosis in Harpagophytum procumbens by changing the cytokinin types, calcium and boron concentrations in the medium (en)
skos:notation	RIV/61989592:15310/09:00010627!RIV10-MSM-15310___
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA522/06/0108), Z(AV0Z50380511), Z(MSM6198959216)
http://linked.open...iv/cisloPeriodika	1
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Doležal, Karel
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Palackého v Olomouci / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	342323
http://linked.open...ai/riv/idVysledku	RIV/61989592:15310/09:00010627
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Boron; Calcium; Cytokinins; Harpagophytum procumbens; Regeneration; Shoot-tip necrosis; Topolins (en)
http://linked.open.../riv/klicoveSlovo	Cytokinins Calcium Boron Harpagophytum procumbens Shoot-tip necrosis Regeneration Topolins
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[EE799200B7E1]
http://linked.open...i/riv/nazevZdroje	South African Journal of Botany
http://linked.open...in/vavai/riv/obor	EF
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	5 (xsd:int)
http://linked.open...vavai/riv/projekt	New cytokinin derivatives in plants: isolation, identification and biological activity
http://linked.open...UplatneniVysledku	2009
http://linked.open...v/svazekPeriodika	75
http://linked.open...iv/tvurceVysledku	Doležal, Karel Stirk, Wendy A. Bairu, M. W. Jain, N. Van Staden, Johanes
http://linked.open...n/vavai/riv/zamer	Mechanismy regulace růstu a vývoje rostlin na úrovni buněk, orgánů a celých organismů: fyziologické, genetické a molekulárně biologické základy Modulation of signalling and regulatory pathways in normal and cancer cells
issn	0254-6299
number of pages	6 (xsd:int)
http://localhost/t...ganizacniJednotka	15310

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