About: Subcellular localization and biochemical comparison of cytosolic and secreted cytokinin dehydrogenase enzymes from maize

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Description	The aim was to characterize the maize non-secreted CKX gene and elucidate the ZmCKX10 subcellular localization. For determination of subcellular localization, the CKX genes were fused with green fluorescent protein (GFP) and overexpressed in tomato hairy roots. Using confocal microscopy, ZmCKX1-GFP signal was confirmed to be present in the apoplast, whereas ZmCKX10-GFP was detected in the cytosol. While roots overexpressing ZmCKX1-GFP formed significantly more mass in comparison to the control, non-secreted CKX overexpression resulted in a small reduction in root mass accumulation. Biochemical characterization of ZmCKX10 was performed using recombinant protein produced in Pichia pastoris. In contrast to the preference for 2,6-dichlorophenolindophenol (DCPIP) as an electron acceptor and transzeatin, N6-(Δ2-isopentenyl)adenine (iP) and N6-(Δ 2-isopentenyl)adenosin (iPR) as substrates for ZmCKX1, the non-secreted ZmCKX10 had a range of suitable electron acceptors and different substrate prefere The aim was to characterize the maize non-secreted CKX gene and elucidate the ZmCKX10 subcellular localization. For determination of subcellular localization, the CKX genes were fused with green fluorescent protein (GFP) and overexpressed in tomato hairy roots. Using confocal microscopy, ZmCKX1-GFP signal was confirmed to be present in the apoplast, whereas ZmCKX10-GFP was detected in the cytosol. While roots overexpressing ZmCKX1-GFP formed significantly more mass in comparison to the control, non-secreted CKX overexpression resulted in a small reduction in root mass accumulation. Biochemical characterization of ZmCKX10 was performed using recombinant protein produced in Pichia pastoris. In contrast to the preference for 2,6-dichlorophenolindophenol (DCPIP) as an electron acceptor and transzeatin, N6-(Δ2-isopentenyl)adenine (iP) and N6-(Δ 2-isopentenyl)adenosin (iPR) as substrates for ZmCKX1, the non-secreted ZmCKX10 had a range of suitable electron acceptors and different substrate prefere (en)
Title	Subcellular localization and biochemical comparison of cytosolic and secreted cytokinin dehydrogenase enzymes from maize Subcellular localization and biochemical comparison of cytosolic and secreted cytokinin dehydrogenase enzymes from maize (en)
skos:prefLabel	Subcellular localization and biochemical comparison of cytosolic and secreted cytokinin dehydrogenase enzymes from maize Subcellular localization and biochemical comparison of cytosolic and secreted cytokinin dehydrogenase enzymes from maize (en)
skos:notation	RIV/61989592:15310/09:00010322!RIV10-MSM-15310___
http://linked.open...avai/riv/aktivita	P S
http://linked.open...avai/riv/aktivity	P(ME 861), S
http://linked.open...iv/cisloPeriodika	9
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Frébort, Ivo Galuszka, Petr
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Palackého v Olomouci / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	344601
http://linked.open...ai/riv/idVysledku	RIV/61989592:15310/09:00010322
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Apoplast; CKX; CKX activity assay; cytokinin; cytosol; GFP; Pichia pastoris expression system; subcellular localization; tomato hairy root transformation; Zea mays. (en)
http://linked.open.../riv/klicoveSlovo	cytokinin GFP subcellular localization Pichia pastoris expression system Apoplast Zea mays. cytosol tomato hairy root transformation CKX CKX activity assay
http://linked.open...odStatuVydavatele	GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV	[FBDCB21F46FC]
http://linked.open...i/riv/nazevZdroje	Journal of Experimenal Botany
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	9 (xsd:int)
http://linked.open...vavai/riv/projekt	http://linked.opendata.cz/resource/domain/vavai/projekt/ME%20861
http://linked.open...UplatneniVysledku	2009
http://linked.open...v/svazekPeriodika	60
http://linked.open...iv/tvurceVysledku	Sedlářová, Michaela Šebela, Marek Frébort, Ivo Galuszka, Petr Kowalska, Marta Šmehilová, Mária Jaworek, Pavel Bilyeu, Kristin D. English, James T.
issn	0022-0957
number of pages	12 (xsd:int)
http://localhost/t...ganizacniJednotka	15310

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