About: Isolation of novel indole-3-acetic acid conjugates by immunoaffinity extraction

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Isolation of novel indole-3-acetic acid conjugates by immunoaffinity extraction Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	An analytical protocol for the isolation and quantification of indole-3-acetic acid (IAA) and its amino acid conjugates was developed. IAA is an important phytohormone and formation of its conjugates plays a crucial role in regulating IAA levels in plants. The developed protocol combines a highly specific immunoaffinity extraction with a sensitive and selective LC?MS/MS analysis. By using internal standards for each of the studied compounds, IAA and seven amino acid conjugates were analyzed in quantities of fresh plant material as low as 30 mg. In seeds of Helleborus niger, physiological levels of these compounds were found to range from 7.5 nmol g-1 fresh weight (IAA) to 0.44 pmol g-1 fresh weight (conjugate with Ala). To our knowledge, the identification of IAA conjugates with Gly, Phe and Val from higher plants is reported here for the first time. An analytical protocol for the isolation and quantification of indole-3-acetic acid (IAA) and its amino acid conjugates was developed. IAA is an important phytohormone and formation of its conjugates plays a crucial role in regulating IAA levels in plants. The developed protocol combines a highly specific immunoaffinity extraction with a sensitive and selective LC?MS/MS analysis. By using internal standards for each of the studied compounds, IAA and seven amino acid conjugates were analyzed in quantities of fresh plant material as low as 30 mg. In seeds of Helleborus niger, physiological levels of these compounds were found to range from 7.5 nmol g-1 fresh weight (IAA) to 0.44 pmol g-1 fresh weight (conjugate with Ala). To our knowledge, the identification of IAA conjugates with Gly, Phe and Val from higher plants is reported here for the first time. (en)
Title	Isolation of novel indole-3-acetic acid conjugates by immunoaffinity extraction Isolation of novel indole-3-acetic acid conjugates by immunoaffinity extraction (en)
skos:prefLabel	Isolation of novel indole-3-acetic acid conjugates by immunoaffinity extraction Isolation of novel indole-3-acetic acid conjugates by immunoaffinity extraction (en)
skos:notation	RIV/61989592:15310/09:00010197!RIV10-MSM-15310___
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GD522/08/H003), P(KAN200380801), Z(AV0Z50380511), Z(MSM6198959216)
http://linked.open...iv/cisloPeriodika	2
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Barták, Petr Rolčík, Jakub Novák, Ondřej Strnad, Miroslav Buchtík, Roman Pěnčík, Aleš
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Palackého v Olomouci / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	320507
http://linked.open...ai/riv/idVysledku	RIV/61989592:15310/09:00010197
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Immunoaffinity extraction; High-performance liquid chromatography (HPLC); Tandem mass spectrometry (MS/MS); Indole-3-acetic acid (IAA); Auxin conjugates; Helleborus niger L. (en)
http://linked.open.../riv/klicoveSlovo	Tandem mass spectrometry (MS/MS) Helleborus niger L. Immunoaffinity extraction Auxin conjugates High-performance liquid chromatography (HPLC) Indole-3-acetic acid (IAA)
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[6ABE3CB07BEB]
http://linked.open...i/riv/nazevZdroje	Talanta
http://linked.open...in/vavai/riv/obor	CB
http://linked.open...ichTvurcuVysledku	6 (xsd:int)
http://linked.open...cetTvurcuVysledku	8 (xsd:int)
http://linked.open...vavai/riv/projekt	Immunonanotechnologies for hormone diagnostic Integration of doctoral studies in biochemistry, plant physiology and biophysics
http://linked.open...UplatneniVysledku	2009
http://linked.open...v/svazekPeriodika	80
http://linked.open...iv/tvurceVysledku	Barták, Petr Novák, Ondřej Rolčík, Jakub Strnad, Miroslav Buchtík, Roman Pěnčík, Aleš Magnus, Volker Salopek-Sondi, Branka
http://linked.open...n/vavai/riv/zamer	Mechanismy regulace růstu a vývoje rostlin na úrovni buněk, orgánů a celých organismů: fyziologické, genetické a molekulárně biologické základy Modulation of signalling and regulatory pathways in normal and cancer cells
issn	0039-9140
number of pages	5 (xsd:int)
http://localhost/t...ganizacniJednotka	15310

Faceted Search & Find service v1.16.118 as of Jun 21 2024

Alternative Linked Data Documents: ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3240 as of Jun 21 2024, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (126 GB total memory, 58 GB memory in use)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software