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| Description
| - Kvartérní benzo[c]fenanthridinový alkaloid chelerythrin je široce využíván jako inhibitor protein kinasy C (PKC). V biologických systémech ale chelerythrin interaguje s plejádou proteinů. V této studii jsme zkoumali účinky chelerythrinu a sanguinarinu na konvenční PKCs (cPKCs) a PKC upstream kinasu, fosfoinositidedependent protein kinasu 1 (PDK1), za podmínek kompletní inhibice PKC-dependentního oxidativního vzplanutí. V neutrofilům podobných buňkách HL-60 cells, inhibovaly sanguinarin a chelerythrin N-formyl-Met-Leu-Phe, phorbol 12-myristate 13-acetate (PMA)-, a A23187-indukované oxidativní vzplanutí, s hodnotami IC50 nepřesahující 4.6 μmol/L, ale inhibice PMA-stimulované cPKC aktivity v intaktních buňkách vyžadovala nejméně pětkrát vyšší koncentraci alkaloidu. Sanguinarin a chelerythrin bránily fosforylaci 80 kDa proteinu a zadržovaly 60 kDa fosfoproteid v cytosolu v koncentracích pod 10 μmol/L. Navíc ani sanguinarin ani chelerythrin neblokovaly PMA-stimulovanou translokaci a (cs)
- The quaternary benzo[c]phenanthridine alkaloid chelerythrine is widely used as an inhibitor of protein kinase C (PKC). However, in biological systems chelerythrine interacts with an array of proteins. In this study, we examined the effects of chelerythrine and sanguinarine on conventional PKCs (cPKCs) and PKC upstream kinase, phosphoinositide-dependent protein kinase 1 (PDK1), under complete inhibition conditions of PKC-dependent oxidative burst. In neutrophil-like HL-60 cells, sanguinarine and chelerythrine inhibited N-formyl-Met-Leu-Phe, phorbol 12-myristate 13-acetate (PMA)-, and A23187-induced oxidative burst with IC50 values not exceeding 4.6 mu mol/L, but the inhibition of PMA-stimulated cPKC activity in intact cells required at least fivefold higher alkaloid concentrations. At concentrations below 10 mu mol/L, sanguinarine and chelerythrine prevented phosphorylation of similar to 80 kDa protein and sequestered similar to 60 kDa phosphoprotein in cytosol. Moreover, neither sanguinarine nor chele
- The quaternary benzo[c]phenanthridine alkaloid chelerythrine is widely used as an inhibitor of protein kinase C (PKC). However, in biological systems chelerythrine interacts with an array of proteins. In this study, we examined the effects of chelerythrine and sanguinarine on conventional PKCs (cPKCs) and PKC upstream kinase, phosphoinositide-dependent protein kinase 1 (PDK1), under complete inhibition conditions of PKC-dependent oxidative burst. In neutrophil-like HL-60 cells, sanguinarine and chelerythrine inhibited N-formyl-Met-Leu-Phe, phorbol 12-myristate 13-acetate (PMA)-, and A23187-induced oxidative burst with IC50 values not exceeding 4.6 mu mol/L, but the inhibition of PMA-stimulated cPKC activity in intact cells required at least fivefold higher alkaloid concentrations. At concentrations below 10 mu mol/L, sanguinarine and chelerythrine prevented phosphorylation of similar to 80 kDa protein and sequestered similar to 60 kDa phosphoprotein in cytosol. Moreover, neither sanguinarine nor chele (en)
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| Title
| - Conventional protein kinase C isoenzymes undergo dephosphorylation in neutrophil-like HL-60 cells treated by chelerythrine or sanguinarine
- Conventional protein kinase C isoenzymes undergo dephosphorylation in neutrophil-like HL-60 cells treated by chelerythrine or sanguinarine (en)
- Isoenzymy konvenční protein dinasy C podléhají defosforylaci v neutrofilům podobných HL-60 buňkách inkubovaných s chelerythrinem a sanguinarinem. (cs)
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| skos:prefLabel
| - Conventional protein kinase C isoenzymes undergo dephosphorylation in neutrophil-like HL-60 cells treated by chelerythrine or sanguinarine
- Conventional protein kinase C isoenzymes undergo dephosphorylation in neutrophil-like HL-60 cells treated by chelerythrine or sanguinarine (en)
- Isoenzymy konvenční protein dinasy C podléhají defosforylaci v neutrofilům podobných HL-60 buňkách inkubovaných s chelerythrinem a sanguinarinem. (cs)
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| skos:notation
| - RIV/61989592:15310/08:00005343!RIV09-MSM-15310___
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
| - P(GP303/06/P193), Z(MSM6198959216)
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/61989592:15310/08:00005343
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - chelerythrine; HL-60 cells; oxidative burst; phosphoinositide-dependent protein kinase 1; protein kinase C; sanguinarine (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...odStatuVydavatele
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
| - Cell Biology and Toxicology
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| http://linked.open...in/vavai/riv/obor
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| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...vavai/riv/projekt
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| http://linked.open...UplatneniVysledku
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| http://linked.open...v/svazekPeriodika
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| http://linked.open...iv/tvurceVysledku
| - Dvořák, Zdeněk
- Ulrichová, Jitka
- Vrba, Jiří
- Modrianský, Martin
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| http://linked.open...n/vavai/riv/zamer
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| issn
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| number of pages
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| http://localhost/t...ganizacniJednotka
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