About: Capillary electrophoretic method for nucleotide analysis in cells: Application on inherited metabolic disorders

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About: Capillary electrophoretic method for nucleotide analysis in cells: Application on inherited metabolic disorders Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Purine and pyrimidine nucleotides influence many metabolic pathways and their analogs have been widely used in medicine. A capillary electrophoretic method was developed for measuring intracellular nucleotides. The final BGE consisted of 40 mM citric acid with addition of 0.8 mM CTAB titrated by gamma-aminobutyric acid to pH 4.4. The electrophoretic separations were carried out in an uncoated silica capillary (id/od - 75/375 mu m; effective/total length - 90/97 cm). The method allows a complete separation of 21 nucleotides and deoxynucleotides within 15 min with separation efficiencies up to 400 000 theoretical plates per meter. Due to the use of an acidic separation medium, the method offers a high selectivity toward the studied analytes versus possible interferences from matrices. Sample preparation was optimized in order to shorten work-time and prevent analyte degradation. The method was applied for analyzing nucleotides in human erythrocytes and Chinese hamster ovary cells. Diagnostic potential f Purine and pyrimidine nucleotides influence many metabolic pathways and their analogs have been widely used in medicine. A capillary electrophoretic method was developed for measuring intracellular nucleotides. The final BGE consisted of 40 mM citric acid with addition of 0.8 mM CTAB titrated by gamma-aminobutyric acid to pH 4.4. The electrophoretic separations were carried out in an uncoated silica capillary (id/od - 75/375 mu m; effective/total length - 90/97 cm). The method allows a complete separation of 21 nucleotides and deoxynucleotides within 15 min with separation efficiencies up to 400 000 theoretical plates per meter. Due to the use of an acidic separation medium, the method offers a high selectivity toward the studied analytes versus possible interferences from matrices. Sample preparation was optimized in order to shorten work-time and prevent analyte degradation. The method was applied for analyzing nucleotides in human erythrocytes and Chinese hamster ovary cells. Diagnostic potential f (en)
Title	Capillary electrophoretic method for nucleotide analysis in cells: Application on inherited metabolic disorders Capillary electrophoretic method for nucleotide analysis in cells: Application on inherited metabolic disorders (en)
skos:prefLabel	Capillary electrophoretic method for nucleotide analysis in cells: Application on inherited metabolic disorders Capillary electrophoretic method for nucleotide analysis in cells: Application on inherited metabolic disorders (en)
skos:notation	RIV/61989592:15310/07:00005810!RIV10-MSM-15310___
http://linked.open...avai/riv/aktivita	P S Z
http://linked.open...avai/riv/aktivity	P(NR7796), P(NR8578), S, Z(MSM6198959205)
http://linked.open...iv/cisloPeriodika	3
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Maier, Vítězslav Adam, Tomáš Friedecký, David
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Palackého v Olomouci / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	412605
http://linked.open...ai/riv/idVysledku	RIV/61989592:15310/07:00005810
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	CE; inborn errors; MEKC; metabolism; nucleotide (en)
http://linked.open.../riv/klicoveSlovo	metabolism MEKC inborn errors CE nucleotide
http://linked.open...odStatuVydavatele	DE - Spolková republika Německo
http://linked.open...ontrolniKodProRIV	[088DC7AA3B80]
http://linked.open...i/riv/nazevZdroje	Electrophoresis
http://linked.open...in/vavai/riv/obor	CB
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...vavai/riv/projekt	PURINE DE NOVO SYNTHESIS DEFECTS - METABOLIC STUDY The occurence of the inherited metabolic disorders in early spontaneous abortions
http://linked.open...UplatneniVysledku	2007
http://linked.open...v/svazekPeriodika	28
http://linked.open...iv/tvurceVysledku	Janošťáková, Anna Procházka, Martin Maier, Vítězslav Adam, Tomáš Friedecký, David Tomková, Jana
http://linked.open...n/vavai/riv/zamer	Studies of genes and molecular mechanisms involved in regulation of hematopoiesis, their clinical impact and targeted treatment
issn	0173-0835
number of pages	8 (xsd:int)
http://localhost/t...ganizacniJednotka	15310

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