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| rdf:type
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| Description
| - Byli připraveny polyklonální protilátky proti 24-epicastasteronu. Protilátky byly připraveny imunizací králiků pomocí konjugátu 24-epicastasterone O-(carboxymethyl)oxime (24-epiCS-CMO) s hovězím sérovým albuminem (BSA). Dané protilátky byly použity pro vývoj ELISA testu pro stanoveni 24-epicastasteronu a příbuzných brssinosteroidů s detekčním limitem od 0.005 do 50 pmolů. Pro analýzu endogenních BRs byly použity rostliny fazole (Phaseolus vulgaris L., cv. Pinto), mrkve (Daucus carota ssp.sativus), Arabidopsis thaliana L. Heynh. A řepkový pyl (Brassica napus L.). Stanovení bylo prováděno pomocí HPLC-ELISA a HPLC-MS. (cs)
- We have developed enzyme-linked immunosorbent assays (ELISAs) for measuring 24-epicastasterone and related brassinolide analogues, with detection ranges of 0.005 to 50 pmoles. Polyclonal antibodies used in these assays were raised against 24-epicastasterone carboxymethyloxime-bovine serum albumin conjugates and were found to have high specificity for 24-epibrassinosteroids. Natural brassinosteroids (BRs), such as brassinolide and 24-epibrassinolide, exhibited relatively high cross-reactivities with the generated antibodies, while other BR analogues with β-oriented hydroxyl groups at C-2, C-3, C-22, and C23 lacked immunoreactivity. Through the use of internal standardization, dilution assays, recovery of authentic [3H]24-epicastasterone, and immunohistograms, the ELISAs have been shown to be applicable for estimating 24-epibrassinosteroids levels in crude plant extracts. In order to analyze brassinosteroids in tissues from young bean (Phaseolus vulgaris L., cv. Pinto), Daucus carota ssp.sativus an
- We have developed enzyme-linked immunosorbent assays (ELISAs) for measuring 24-epicastasterone and related brassinolide analogues, with detection ranges of 0.005 to 50 pmoles. Polyclonal antibodies used in these assays were raised against 24-epicastasterone carboxymethyloxime-bovine serum albumin conjugates and were found to have high specificity for 24-epibrassinosteroids. Natural brassinosteroids (BRs), such as brassinolide and 24-epibrassinolide, exhibited relatively high cross-reactivities with the generated antibodies, while other BR analogues with β-oriented hydroxyl groups at C-2, C-3, C-22, and C23 lacked immunoreactivity. Through the use of internal standardization, dilution assays, recovery of authentic [3H]24-epicastasterone, and immunohistograms, the ELISAs have been shown to be applicable for estimating 24-epibrassinosteroids levels in crude plant extracts. In order to analyze brassinosteroids in tissues from young bean (Phaseolus vulgaris L., cv. Pinto), Daucus carota ssp.sativus an (en)
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| Title
| - New technologies for estimation of naturally occuring brassinosteroids
- New technologies for estimation of naturally occuring brassinosteroids (en)
- Nová technologie pro stanovení přirozených brassinosteroidů (cs)
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| skos:prefLabel
| - New technologies for estimation of naturally occuring brassinosteroids
- New technologies for estimation of naturally occuring brassinosteroids (en)
- Nová technologie pro stanovení přirozených brassinosteroidů (cs)
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| skos:notation
| - RIV/61989592:15310/07:00004648!RIV08-MSM-15310___
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| http://linked.open.../vavai/riv/strany
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/61989592:15310/07:00004648
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - brassinosteroids; antibodies; ELISA; rape pollen; HPLC-MS; Arabidopsis (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...odStatuVydavatele
| - DE - Spolková republika Německo
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
| - Journal of Plant Growth Regulation
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| http://linked.open...in/vavai/riv/obor
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| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...UplatneniVysledku
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| http://linked.open...v/svazekPeriodika
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| http://linked.open...iv/tvurceVysledku
| - Kohout, Ladislav
- Novák, Ondřej
- Strnad, Miroslav
- Šíša, Miroslav
- Hauserová, Eva
- Swaczynová, Jana
- Fuksová, Květa
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| http://linked.open...n/vavai/riv/zamer
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| issn
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| number of pages
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| http://localhost/t...ganizacniJednotka
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