About: Tumour suppressor PTEN regulates cell cycle and protein kinase B/Akt pathway in breast cancer cells

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About: Tumour suppressor PTEN regulates cell cycle and protein kinase B/Akt pathway in breast cancer cells Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Background: PTEN is a tumour suppressor protein with phosphatase activity frequently altered in several types of human cancers. Materials and Methods: The PTEN effect was studied on the cell cycle (by bromdeoxyuridine incorporation) and on the phosphatidylinositol-3-kinaselprotein kinase B/Akt (PI3-K/PKB/Akt) pathway regulating proteins (by immunocytochemical, Western blot analysis and kinase assay) upon transfection of wild-type PTEN and its mutant H123Y in breast cancer cell lines. Results: The expression of the important proteins in the MCF-7 and BT-549 cells was characterised and the cellular localisation of PTEN was analysed. Transfection of H123Y led to the down-regulation of p27(KiP1) and p21(Waf1/Cip1) protein levels and the up-regulation of phosphorylated PKB/Akt. An overexpression of PTEN decreased cyclin E/cdk2 activity and inhibited S-phase entry in MCF-7. In BT-549 these changes were not observed, but overexpression of PTEN led to a diminution of PKB/Akt phosphorylation. Conclusion: PTEN Background: PTEN is a tumour suppressor protein with phosphatase activity frequently altered in several types of human cancers. Materials and Methods: The PTEN effect was studied on the cell cycle (by bromdeoxyuridine incorporation) and on the phosphatidylinositol-3-kinaselprotein kinase B/Akt (PI3-K/PKB/Akt) pathway regulating proteins (by immunocytochemical, Western blot analysis and kinase assay) upon transfection of wild-type PTEN and its mutant H123Y in breast cancer cell lines. Results: The expression of the important proteins in the MCF-7 and BT-549 cells was characterised and the cellular localisation of PTEN was analysed. Transfection of H123Y led to the down-regulation of p27(KiP1) and p21(Waf1/Cip1) protein levels and the up-regulation of phosphorylated PKB/Akt. An overexpression of PTEN decreased cyclin E/cdk2 activity and inhibited S-phase entry in MCF-7. In BT-549 these changes were not observed, but overexpression of PTEN led to a diminution of PKB/Akt phosphorylation. Conclusion: PTEN (en)
Title	Tumour suppressor PTEN regulates cell cycle and protein kinase B/Akt pathway in breast cancer cells Tumour suppressor PTEN regulates cell cycle and protein kinase B/Akt pathway in breast cancer cells (en)
skos:prefLabel	Tumour suppressor PTEN regulates cell cycle and protein kinase B/Akt pathway in breast cancer cells Tumour suppressor PTEN regulates cell cycle and protein kinase B/Akt pathway in breast cancer cells (en)
skos:notation	RIV/61989592:15310/06:00010655!RIV10-MSM-15310___
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA204/01/0488), P(NC6779), P(NR7828), Z(AV0Z50380511), Z(MSM6198959216)
http://linked.open...iv/cisloPeriodika	2A
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Hlobilková, Alice Kryštof, Vladimír Knillová, Jana KOLÁŘ, Zdeněk Šváchová, Michaela
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Palackého v Olomouci / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	504532
http://linked.open...ai/riv/idVysledku	RIV/61989592:15310/06:00010655
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	breast cancer cell lines; cell cycle; phosphatase activity; PKB/Akt; PTEN; tumour suppressor; p27(Kip1); p21(Waf1/Cip1); p53 and pRb (en)
http://linked.open.../riv/klicoveSlovo	phosphatase activity cell cycle breast cancer cell lines p21(Waf1/Cip1) p27(Kip1) p53 and pRb PKB/Akt tumour suppressor PTEN
http://linked.open...odStatuVydavatele	GR - Řecká republika
http://linked.open...ontrolniKodProRIV	[DA1BB8952A68]
http://linked.open...i/riv/nazevZdroje	Anticancer Research
http://linked.open...in/vavai/riv/obor	FD
http://linked.open...ichTvurcuVysledku	5 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...vavai/riv/projekt	Analysis of PTEN gene interactions in prostate cancer cell lines Study of importance of proteins regulating PI3-K/PKB/Akt signaling pathway and cell cycle for glioblastoma pathogenesis Study of molecular mechanisms affecting phosphatase activity of PTEN gene product
http://linked.open...UplatneniVysledku	2006
http://linked.open...v/svazekPeriodika	26
http://linked.open...iv/tvurceVysledku	Kryštof, Vladimír Kolář, Zdeněk Šváchová, Michaela Hlobilková, Alice Knillová, Jana Skypalová, Petra
http://linked.open...n/vavai/riv/zamer	Mechanismy regulace růstu a vývoje rostlin na úrovni buněk, orgánů a celých organismů: fyziologické, genetické a molekulárně biologické základy Modulation of signalling and regulatory pathways in normal and cancer cells
issn	0250-7005
number of pages	8 (xsd:int)
http://localhost/t...ganizacniJednotka	15310

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