About: Occurrence of two 5-aminolevulinate biosynthetic pathways in Streptomyces nodosus subsp asukaensis is linked with the production of asukamycin

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About: Occurrence of two 5-aminolevulinate biosynthetic pathways in Streptomyces nodosus subsp asukaensis is linked with the production of asukamycin Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	We report the results of cloning genes for two key biosynthetic enzymes of different 5-aminolevulinic acid (ALA) biosynthetic routes from Streptomyces. The genes encode the glutamyl-tRNA(Glu) reductase (GluTR) of the C. pathway and the ALA synthase (ALAS) of the Shemin pathway. While Streptomyces coelicolor A3(2) synthesizes ALA via the C, route, both pathways are operational in Streptomyces nodosus subsp. asukaensis, a producer of asukamycin. In this strain, the C. route produces ALA for tetrapyrrole biosynthesis; the ALA formed by the Shemin pathway serves as a precursor of the 2-amino-3-hydroxycyclopent-2-enone moiety (C5N unit), an antibiotic component. The growth of S. nodosus and S. coelicolor strains deficient in the GluTR genes (gtr) is strictly dependent on ALA or heme supplementation, whereas the defect in the ALAS-encoding gene (hemA-asuA) abolishes the asukamycin production in S. nodosus. The recombinant hemA-asuA gene was expressed in Escherichia coli and in Streptomyces, and the encoded We report the results of cloning genes for two key biosynthetic enzymes of different 5-aminolevulinic acid (ALA) biosynthetic routes from Streptomyces. The genes encode the glutamyl-tRNA(Glu) reductase (GluTR) of the C. pathway and the ALA synthase (ALAS) of the Shemin pathway. While Streptomyces coelicolor A3(2) synthesizes ALA via the C, route, both pathways are operational in Streptomyces nodosus subsp. asukaensis, a producer of asukamycin. In this strain, the C. route produces ALA for tetrapyrrole biosynthesis; the ALA formed by the Shemin pathway serves as a precursor of the 2-amino-3-hydroxycyclopent-2-enone moiety (C5N unit), an antibiotic component. The growth of S. nodosus and S. coelicolor strains deficient in the GluTR genes (gtr) is strictly dependent on ALA or heme supplementation, whereas the defect in the ALAS-encoding gene (hemA-asuA) abolishes the asukamycin production in S. nodosus. The recombinant hemA-asuA gene was expressed in Escherichia coli and in Streptomyces, and the encoded (en)
Title	Occurrence of two 5-aminolevulinate biosynthetic pathways in Streptomyces nodosus subsp asukaensis is linked with the production of asukamycin Occurrence of two 5-aminolevulinate biosynthetic pathways in Streptomyces nodosus subsp asukaensis is linked with the production of asukamycin (en)
skos:prefLabel	Occurrence of two 5-aminolevulinate biosynthetic pathways in Streptomyces nodosus subsp asukaensis is linked with the production of asukamycin Occurrence of two 5-aminolevulinate biosynthetic pathways in Streptomyces nodosus subsp asukaensis is linked with the production of asukamycin (en)
skos:notation	RIV/61989592:15310/06:00010634!RIV10-MSM-15310___
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA310/03/0285), P(KJB5020306), P(ME 707), Z(AV0Z50200510), Z(AV0Z50380511), Z(MSM6198959216)
http://linked.open...iv/cisloPeriodika	14
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Havlíček, Libor
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Palackého v Olomouci / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	489820
http://linked.open...ai/riv/idVysledku	RIV/61989592:15310/06:00010634
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	transfer-RNA reductase; complete genome sequence; acid synthase; Escherichia-coli; rhodobacter-sphaeroides; bacillus-subtilis; coelicolor A3(2); hema gene; Euglena-gracilis; cloning (en)
http://linked.open.../riv/klicoveSlovo	Escherichia-coli Euglena-gracilis acid synthase coelicolor A3(2) complete genome sequence hema gene transfer-RNA reductase bacillus-subtilis cloning rhodobacter-sphaeroides
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[049C3464E306]
http://linked.open...i/riv/nazevZdroje	Journal of Bacteriology
http://linked.open...in/vavai/riv/obor	EE
http://linked.open...ichTvurcuVysledku	1 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...vavai/riv/projekt	http://linked.opendata.cz/resource/domain/vavai/projekt/ME%20707 Analysis of gene clusters involved in biosynthesis of manumycin-type metabolites Biosynthesis of asukamycin polyketide chains: Prospects for hybrid antibotics
http://linked.open...UplatneniVysledku	2006
http://linked.open...v/svazekPeriodika	188
http://linked.open...iv/tvurceVysledku	Havlíček, Libor Felsberg, Jürgen Petříček, Miroslav Petříčková, Kateřina
http://linked.open...n/vavai/riv/zamer	Microorganisms in Research and Biotechnology Mechanismy regulace růstu a vývoje rostlin na úrovni buněk, orgánů a celých organismů: fyziologické, genetické a molekulárně biologické základy Modulation of signalling and regulatory pathways in normal and cancer cells
issn	0021-9193
number of pages	11 (xsd:int)
http://localhost/t...ganizacniJednotka	15310

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