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| Description
| - Rychlá procedura založená na solid-phase extrakci a HPLC stanovení linkomycinu v premixech s UV detekcí je v práci popsána. Po extrakci linkomycinu pomocí extrakčního činidla je extrakt nastříknut na OASIS HLB kolonu. Linkomycin je eluován metanolem a eluent je analyzován na analytické koloně s použitím mobilní fáze složené z 0,2 % kyseliny fosforečné ve směsi vody a acetonitrilu. Detekce je prováděna při 208 nm.Ke kvantifikaci se používá metoda vnějšího standardu. Průměrná výtěžnost byla 105,0 +/- 7,3 % v koncentračním rozmezí 250-750 mg/kg a 99,8 +/- 3,7 % v koncentračním rozmezí 10000-150000 mg/kg. Mez detekce určená poměrem signál ku šumu 10:1 byla 5,2 mg/kg. LC-MS/MS konfirmace linkomycinu je také prezentována. (cs)
- A rapid clean-up procedure based on solid-phase extraction (SPE) and HPLC determination of lincomycin in premixes with UV detection is described. After extraction of lincomycin from premix with extraction solvent the extract is applied to OASIS HLB column treated with methanol and water. Lincomycin is eluted with methanol and effluent is analysed on analytical column (phenyl) using mobile phase consists 0.2% phosphoric acid in water and acetonitrile (875:125, v/v). Detection is performed at 208 nm. Quantitation is carried out using external standard. The mean recovery of lincomycin was 105.0 +/- 7.3%, in concentration range of 250-750 mg k,(-1), and 99.8 +/- 3.7%, in concentration range of 10,000-150,000mg kg(-1). The limit of determination, based on a signal-to-noise ratio of 10:1, was 5.2mg kg(-1). LC-MS/MS confirmation of lincomycin is also presented. Identification was performed by monitoring two pairs of multiple reaction monitoring ions from the parent ions (m/z 407.2 -> 126.1 and 407.2 -
- A rapid clean-up procedure based on solid-phase extraction (SPE) and HPLC determination of lincomycin in premixes with UV detection is described. After extraction of lincomycin from premix with extraction solvent the extract is applied to OASIS HLB column treated with methanol and water. Lincomycin is eluted with methanol and effluent is analysed on analytical column (phenyl) using mobile phase consists 0.2% phosphoric acid in water and acetonitrile (875:125, v/v). Detection is performed at 208 nm. Quantitation is carried out using external standard. The mean recovery of lincomycin was 105.0 +/- 7.3%, in concentration range of 250-750 mg k,(-1), and 99.8 +/- 3.7%, in concentration range of 10,000-150,000mg kg(-1). The limit of determination, based on a signal-to-noise ratio of 10:1, was 5.2mg kg(-1). LC-MS/MS confirmation of lincomycin is also presented. Identification was performed by monitoring two pairs of multiple reaction monitoring ions from the parent ions (m/z 407.2 -> 126.1 and 407.2 - (en)
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| Title
| - HPLC determination of lincomycin in premixes and feedstuffs with solid-phase extraction on HLB OASIS and LC-MS/MS confirmation
- HPLC stanovení linkomycinu v premixech a krmných směsích s použitím solid-phase extrakce na HLB OASIS a LC-MS/MS potvrzení (cs)
- HPLC determination of lincomycin in premixes and feedstuffs with solid-phase extraction on HLB OASIS and LC-MS/MS confirmation (en)
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| skos:prefLabel
| - HPLC determination of lincomycin in premixes and feedstuffs with solid-phase extraction on HLB OASIS and LC-MS/MS confirmation
- HPLC stanovení linkomycinu v premixech a krmných směsích s použitím solid-phase extrakce na HLB OASIS a LC-MS/MS potvrzení (cs)
- HPLC determination of lincomycin in premixes and feedstuffs with solid-phase extraction on HLB OASIS and LC-MS/MS confirmation (en)
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| skos:notation
| - RIV/61989592:15310/06:00003365!RIV07-MSM-15310___
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| http://linked.open.../vavai/riv/strany
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/61989592:15310/06:00003365
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - HPLC determination; LC-MS/MS confirmation; lincomycin; premix; validation (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...odStatuVydavatele
| - GB - Spojené království Velké Británie a Severního Irska
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
| - Journal of Pharmaceutical and Biomedical Analysis
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| http://linked.open...in/vavai/riv/obor
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| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...UplatneniVysledku
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| http://linked.open...v/svazekPeriodika
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| http://linked.open...iv/tvurceVysledku
| - Lemr, Karel
- Douša, Michal
- Halama, Michal
- Sikač, Zdeněk
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| http://linked.open...n/vavai/riv/zamer
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| issn
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| number of pages
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| http://localhost/t...ganizacniJednotka
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