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  • It has been suggested that both free metals and reduced ferredoxin (Fd) participate in the light-induced production of hydroxyl radicals (OH.) in thylakoid membranes of chloroplasts. The most direct evidence of the involvement of Fd in OH. formation under physiological conditions was reported by Jakob and Heber (Plant Cell Physiol., 1996, 37, 629-635), who used the oxidation of dimethylsulfoxide to methane sulfinic acid as an indicator of OH. production. We confirmed their conclusions using a more sensitive and reliable EPR spin-trapping method and extended their work by additional findings. Free metal-dependent and ferredoxin-dependent OH. production was studied simultaneously and strong metal chelator Desferal was used to distinguish between these reaction pathways. The participation of protein-bound iron within photosystem I was confirmed by partial suppression of OH. generation in broken chloroplasts by methyl viologen. The stimulation of the production of OH. in thylakoid membranes by externally
  • It has been suggested that both free metals and reduced ferredoxin (Fd) participate in the light-induced production of hydroxyl radicals (OH.) in thylakoid membranes of chloroplasts. The most direct evidence of the involvement of Fd in OH. formation under physiological conditions was reported by Jakob and Heber (Plant Cell Physiol., 1996, 37, 629-635), who used the oxidation of dimethylsulfoxide to methane sulfinic acid as an indicator of OH. production. We confirmed their conclusions using a more sensitive and reliable EPR spin-trapping method and extended their work by additional findings. Free metal-dependent and ferredoxin-dependent OH. production was studied simultaneously and strong metal chelator Desferal was used to distinguish between these reaction pathways. The participation of protein-bound iron within photosystem I was confirmed by partial suppression of OH. generation in broken chloroplasts by methyl viologen. The stimulation of the production of OH. in thylakoid membranes by externally (en)
  • It has been suggested that both free metals and reduced ferredoxin (Fd) participate in the light-induced production of hydroxyl radicals (OH.) in thylakoid membranes of chloroplasts. The most direct evidence of the involvement of Fd in OH. formation under physiological conditions was reported by Jakob and Heber (Plant Cell Physiol., 1996, 37, 629-635), who used the oxidation of dimethylsulfoxide to methane sulfinic acid as an indicator of OH. production. We confirmed their conclusions using a more sensitive and reliable EPR spin-trapping method and extended their work by additional findings. Free metal-dependent and ferredoxin-dependent OH. production was studied simultaneously and strong metal chelator Desferal was used to distinguish between these reaction pathways. The participation of protein-bound iron within photosystem I was confirmed by partial suppression of OH. generation in broken chloroplasts by methyl viologen. The stimulation of the production of OH. in thylakoid membranes by externally (cs)
Title
  • Reaction pathways involved in the production of hydroxyl radicals in thylakoid membrane: EPR spin-trapping study.
  • Reaction pathways involved in the production of hydroxyl radicals in thylakoid membrane: EPR spin-trapping study. (en)
  • Reakční cesty produkce hydroxylových radikálů v thylakoidní membráně: spin-trapping EPR studie. (cs)
skos:prefLabel
  • Reaction pathways involved in the production of hydroxyl radicals in thylakoid membrane: EPR spin-trapping study.
  • Reaction pathways involved in the production of hydroxyl radicals in thylakoid membrane: EPR spin-trapping study. (en)
  • Reakční cesty produkce hydroxylových radikálů v thylakoidní membráně: spin-trapping EPR studie. (cs)
skos:notation
  • RIV/61989592:15310/06:00003237!RIV07-MSM-15310___
http://linked.open.../vavai/riv/strany
  • 472-476
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  • P(1K04103)
http://linked.open...iv/cisloPeriodika
  • 5
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  • 496483
http://linked.open...ai/riv/idVysledku
  • RIV/61989592:15310/06:00003237
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • PHOTOSYSTEM-I; OXYGEN; CHLOROPLASTS; FERREDOXIN; PLANTS; IRON; NICOTIANAMINE; TRANSPORT; MECHANISM; LEAVES (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV
  • [8B8E3925F18A]
http://linked.open...i/riv/nazevZdroje
  • Photochemical and Photobiological Sciences
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
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http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 5
http://linked.open...iv/tvurceVysledku
  • Pospíšil, Pavel
  • Nauš, Jan
  • Šnyrychová, Iva
issn
  • 1474-905X
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http://localhost/t...ganizacniJednotka
  • 15310
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