About: Proteolytic enzymes: Significance for proteomics

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Proteolytic enzymes (peptidases) are important tools for proteomic research. This article reviews their application to digestion of proteins, which commonly precedes the mass spectrometry analysis of proteins. In the opening part, mechanism of proteolysis is described together with a detailed introduction to classification and nomenclature of enzymes. The following part brings an overview of proteolytic enzymes that are currently used in expression and functional proteomics including their properties and specificity. The review continues with reports on assays of proteolytic activity and computer prediction of peptide cleavage. The methodology of in-solution and in-gel digestion of samples and the use of peptidases for cleavage of proteins on membrane surfaces is also mentioned. Furthermore, chemical modifications of peptidases for improving their stability to autolysis and thermal inactivation are documented. Peptidase treatment of hydrophobic proteins in solutions containing organic solvents are als Proteolytic enzymes (peptidases) are important tools for proteomic research. This article reviews their application to digestion of proteins, which commonly precedes the mass spectrometry analysis of proteins. In the opening part, mechanism of proteolysis is described together with a detailed introduction to classification and nomenclature of enzymes. The following part brings an overview of proteolytic enzymes that are currently used in expression and functional proteomics including their properties and specificity. The review continues with reports on assays of proteolytic activity and computer prediction of peptide cleavage. The methodology of in-solution and in-gel digestion of samples and the use of peptidases for cleavage of proteins on membrane surfaces is also mentioned. Furthermore, chemical modifications of peptidases for improving their stability to autolysis and thermal inactivation are documented. Peptidase treatment of hydrophobic proteins in solutions containing organic solvents are als (en)
Title	Proteolytic enzymes: Significance for proteomics Proteolytic enzymes: Significance for proteomics (en)
skos:prefLabel	Proteolytic enzymes: Significance for proteomics Proteolytic enzymes: Significance for proteomics (en)
skos:notation	RIV/61989592:15310/05:00010650!RIV10-MSM-15310___
http://linked.open...avai/riv/aktivita	Z
http://linked.open...avai/riv/aktivity	Z(MSM6198959216)
http://linked.open...iv/cisloPeriodika	12
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Štosová, Taťána Šebela, Marek Lenobel, René
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Palackého v Olomouci / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	539384
http://linked.open...ai/riv/idVysledku	RIV/61989592:15310/05:00010650
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	tandem mass-spectrometry; amino-acid sequence; protein identification; polyacrylamide-gels; quadrupole-time; lysobacter-enzymogenes; chemical-modification; carboxypeptidase-Y; pseudomonas-fragi; membrane-proteins (en)
http://linked.open.../riv/klicoveSlovo	membrane-proteins tandem mass-spectrometry protein identification amino-acid sequence carboxypeptidase-Y chemical-modification lysobacter-enzymogenes polyacrylamide-gels pseudomonas-fragi quadrupole-time
http://linked.open...odStatuVydavatele	CZ - Česká republika
http://linked.open...ontrolniKodProRIV	[B1E78F703D8B]
http://linked.open...i/riv/nazevZdroje	Chemické listy
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...UplatneniVysledku	2005
http://linked.open...v/svazekPeriodika	99
http://linked.open...iv/tvurceVysledku	Šebela, Marek Štosová, Taťána Lenobel, René Havliś, Jan
http://linked.open...n/vavai/riv/zamer	Modulation of signalling and regulatory pathways in normal and cancer cells
issn	0009-2770
number of pages	10 (xsd:int)
http://localhost/t...ganizacniJednotka	15310

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