About: The involvement of cytokinin oxidase/dehydrogenase and zeatin reductase in regulation of cytokinin levels in pea (Pisum sativum L.) leaves     Goto   Sponge   NotDistinct   Permalink

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Description
  • Cytokinin metabolism in plants is very complex. More than 20 cytokinins bearing isoprenoid and aromatic side chains were identified by high performance liquid chromatography-mass spectrometry (HPLC-MS) in pea ( Pisum sativum L. cv. Gotik) leaves, indicating diverse metabolic conversions of primary products of cytokinin biosynthesis. To determine the potential involvement of two enzymes metabolizing cytokinins, cytokinin oxidase/dehydrogenase (CKX, EC 1.5.99.12) and zeatin reductase (ZRED, EC 1.3.1.69), in the control of endogenous cytokinin levels, their in vitro activities were investigated in relation to the uptake and metabolism of [2-H-3] trans- zeatin ([2-H-3] Z) in shoot explants of pea. Trans-zeatin 9-riboside, trans-zeatin 9-riboside-5'-monophosphate and cytokinin degradation products adenine and adenosine were detected as predominant [2-H-3] Z metabolites during 2, 5, 8, and 24 h incubation. Increasing formation of adenine and adenosine indicated extensive degradation of [2-H-3] Z by CKX
  • Cytokinin metabolism in plants is very complex. More than 20 cytokinins bearing isoprenoid and aromatic side chains were identified by high performance liquid chromatography-mass spectrometry (HPLC-MS) in pea ( Pisum sativum L. cv. Gotik) leaves, indicating diverse metabolic conversions of primary products of cytokinin biosynthesis. To determine the potential involvement of two enzymes metabolizing cytokinins, cytokinin oxidase/dehydrogenase (CKX, EC 1.5.99.12) and zeatin reductase (ZRED, EC 1.3.1.69), in the control of endogenous cytokinin levels, their in vitro activities were investigated in relation to the uptake and metabolism of [2-H-3] trans- zeatin ([2-H-3] Z) in shoot explants of pea. Trans-zeatin 9-riboside, trans-zeatin 9-riboside-5'-monophosphate and cytokinin degradation products adenine and adenosine were detected as predominant [2-H-3] Z metabolites during 2, 5, 8, and 24 h incubation. Increasing formation of adenine and adenosine indicated extensive degradation of [2-H-3] Z by CKX (en)
Title
  • The involvement of cytokinin oxidase/dehydrogenase and zeatin reductase in regulation of cytokinin levels in pea (Pisum sativum L.) leaves
  • The involvement of cytokinin oxidase/dehydrogenase and zeatin reductase in regulation of cytokinin levels in pea (Pisum sativum L.) leaves (en)
skos:prefLabel
  • The involvement of cytokinin oxidase/dehydrogenase and zeatin reductase in regulation of cytokinin levels in pea (Pisum sativum L.) leaves
  • The involvement of cytokinin oxidase/dehydrogenase and zeatin reductase in regulation of cytokinin levels in pea (Pisum sativum L.) leaves (en)
skos:notation
  • RIV/61989592:15310/05:00009862!RIV10-MSM-15310___
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(GA206/03/0313), S, Z(AV0Z50380511), Z(MSM6198959216)
http://linked.open...iv/cisloPeriodika
  • 3
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 525647
http://linked.open...ai/riv/idVysledku
  • RIV/61989592:15310/05:00009862
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • Aromatic cytokinin; cis-zeatin; cytokinin; cytokinin oxidase/dehydrogenace; dihydrozeatin; metabolism; pea; trans-zeatin; zeatin reductase (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • DE - Spolková republika Německo
http://linked.open...ontrolniKodProRIV
  • [0F44C6F3A08A]
http://linked.open...i/riv/nazevZdroje
  • Journal of Plant Growth Regulation
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 24
http://linked.open...iv/tvurceVysledku
  • Motyka, Václav
  • Novák, Ondřej
  • Strnad, Miroslav
  • Gaudinová, Alena
  • Friedecký, David
  • Dobrev, Petre I.
  • Šolcová, Blanka
http://linked.open...n/vavai/riv/zamer
issn
  • 0721-7595
number of pages
http://localhost/t...ganizacniJednotka
  • 15310
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