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Description
  • The investigation of hormone-receptor interaction normally needs isolation and extensive purification of the receptor protein or a particular receptor-containing fraction. To bypass these time- and resource-consuming procedures, we have established a live cell-based assay using transgenic bacteria expressing single eukaryotic receptors. Here we describe some biochemical features of the Arabidopsis cytokinin receptor CRE1/AHK4 expressed in Escherichia coli. The data show that the main characteristics of the ligand-receptor interaction, including binding affinity and ligand specificity, can be determined using intact bacteria expressing a functional receptor.
  • The investigation of hormone-receptor interaction normally needs isolation and extensive purification of the receptor protein or a particular receptor-containing fraction. To bypass these time- and resource-consuming procedures, we have established a live cell-based assay using transgenic bacteria expressing single eukaryotic receptors. Here we describe some biochemical features of the Arabidopsis cytokinin receptor CRE1/AHK4 expressed in Escherichia coli. The data show that the main characteristics of the ligand-receptor interaction, including binding affinity and ligand specificity, can be determined using intact bacteria expressing a functional receptor. (en)
Title
  • A live cell hormone-binding assay on transgenic bacteria expressing a eukaryotic receptor protein
  • A live cell hormone-binding assay on transgenic bacteria expressing a eukaryotic receptor protein (en)
skos:prefLabel
  • A live cell hormone-binding assay on transgenic bacteria expressing a eukaryotic receptor protein
  • A live cell hormone-binding assay on transgenic bacteria expressing a eukaryotic receptor protein (en)
skos:notation
  • RIV/61989592:15310/05:00009856!RIV10-MSM-15310___
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • S, Z(MSM6198959216)
http://linked.open...iv/cisloPeriodika
  • 1
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 510885
http://linked.open...ai/riv/idVysledku
  • RIV/61989592:15310/05:00009856
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • hormone; receptor; cytokinin; binding assay; transgenic Escherichia coli (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • GB - Spojené království Velké Británie a Severního Irska
http://linked.open...ontrolniKodProRIV
  • [4026F18F6598]
http://linked.open...i/riv/nazevZdroje
  • Analytical Biochemistry
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 347
http://linked.open...iv/tvurceVysledku
  • Strnad, Miroslav
  • Spíchal, Lukáš
  • Schmülling, Thomas
  • Lomin, Sergey N
  • Romanov, Georgy A
http://linked.open...n/vavai/riv/zamer
issn
  • 0003-2697
number of pages
http://localhost/t...ganizacniJednotka
  • 15310
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