| Attributes | Values |
|---|
| rdf:type
| |
| Description
| - Navázáním zbytků cyklodextrinu k molekule rostlinné aminoxidasy byl připraven modifikovaný enzym, který vykazoval při nezměněné specifitě vysokou odolnost vůči teplotní inaktivaci. (cs)
- Syntheses of conjugates of garden pea (Pisum sativum) and grass pea (Lathyrus sativus) amine oxidases (PSAO and GPAO respectively) with BCD (â-cyclodextrin), performed to improve the thermostability of the enzymes, are described in the present study. Periodate-oxidized BCD reacted with the enzyme proteins via free primary amino groups in a buffered solution containingcyanoborohydride as a reductant. Although the specific activities of PSAO and GPAO partially decreased after modification, Km values determined for the best diamine substrates remained almost unchanged. Both the BCD conjugates could be incubated at 65 ◦C for 30 min without considerable inactivation, and the residual activity remained detectable even after incubation at 75 ◦C. The conjugates contained approx. 30% of neutral sugars. Molecular masses of BCD-PSAO and BCD-GPAO (180 kDa), as estimated by gelpermeation chromatography, were higher compared with the value of 145 kDa for the native enzymes. Thiswas in good corre
- Syntheses of conjugates of garden pea (Pisum sativum) and grass pea (Lathyrus sativus) amine oxidases (PSAO and GPAO respectively) with BCD (â-cyclodextrin), performed to improve the thermostability of the enzymes, are described in the present study. Periodate-oxidized BCD reacted with the enzyme proteins via free primary amino groups in a buffered solution containingcyanoborohydride as a reductant. Although the specific activities of PSAO and GPAO partially decreased after modification, Km values determined for the best diamine substrates remained almost unchanged. Both the BCD conjugates could be incubated at 65 ◦C for 30 min without considerable inactivation, and the residual activity remained detectable even after incubation at 75 ◦C. The conjugates contained approx. 30% of neutral sugars. Molecular masses of BCD-PSAO and BCD-GPAO (180 kDa), as estimated by gelpermeation chromatography, were higher compared with the value of 145 kDa for the native enzymes. Thiswas in good corre (en)
|
| Title
| - Thermostable β-cyclodextrin conjugates of two similar plant amine oxidases and their properties
- Termostabilní β-cyklodextrinové deriváty dvou podobných rostlinných aminoxidas a jejich vlastnosti (cs)
- Thermostable β-cyclodextrin conjugates of two similar plant amine oxidases and their properties (en)
|
| skos:prefLabel
| - Thermostable β-cyclodextrin conjugates of two similar plant amine oxidases and their properties
- Termostabilní β-cyklodextrinové deriváty dvou podobných rostlinných aminoxidas a jejich vlastnosti (cs)
- Thermostable β-cyclodextrin conjugates of two similar plant amine oxidases and their properties (en)
|
| skos:notation
| - RIV/61989592:15310/05:00002262!RIV06-MSM-15310___
|
| http://linked.open.../vavai/riv/strany
| |
| http://linked.open...avai/riv/aktivita
| |
| http://linked.open...avai/riv/aktivity
| - P(ME 664), Z(MSM 153100013)
|
| http://linked.open...iv/cisloPeriodika
| |
| http://linked.open...vai/riv/dodaniDat
| |
| http://linked.open...aciTvurceVysledku
| |
| http://linked.open.../riv/druhVysledku
| |
| http://linked.open...iv/duvernostUdaju
| |
| http://linked.open...titaPredkladatele
| |
| http://linked.open...dnocenehoVysledku
| |
| http://linked.open...ai/riv/idVysledku
| - RIV/61989592:15310/05:00002262
|
| http://linked.open...riv/jazykVysledku
| |
| http://linked.open.../riv/klicovaSlova
| - DIAMINE OXIDASE; PEA-SEEDLINGS; ACTIVE-SITES; PROTEINS; MECHANISM; GLYCOSYLATION; STABILIZATION; INHIBITOR; STABILITY; CLONING (en)
|
| http://linked.open.../riv/klicoveSlovo
| |
| http://linked.open...odStatuVydavatele
| - GB - Spojené království Velké Británie a Severního Irska
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| http://linked.open...ontrolniKodProRIV
| |
| http://linked.open...i/riv/nazevZdroje
| - Biotechnology and Applied Biochemistry
|
| http://linked.open...in/vavai/riv/obor
| |
| http://linked.open...ichTvurcuVysledku
| |
| http://linked.open...cetTvurcuVysledku
| |
| http://linked.open...vavai/riv/projekt
| |
| http://linked.open...UplatneniVysledku
| |
| http://linked.open...v/svazekPeriodika
| |
| http://linked.open...iv/tvurceVysledku
| - Kopečný, David
- Šebela, Marek
- Havliš, Jan
- Lamplot, Zbyněk
- Shevchenko, Andrej
- Thomas, Henrik
|
| http://linked.open...n/vavai/riv/zamer
| |
| issn
| |
| number of pages
| |
| http://localhost/t...ganizacniJednotka
| |
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