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| rdf:type
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| Description
| - Plant copper/topaquinone-containing amine oxidases (CAOs, EC 1.4.3.6) are enzymes oxidising various amines. Here wereport a study on the reactions of CAOs from grass pea (Lathyrus sativus), lentil (Lens esculenta) and Euphorbia characias, aMediterranean shrub, with N6-aminoalkyl adenines representing combined analogues of cytokinins and polyamines. Thefollowing compounds were synthesised: N6-(3-aminopropyl)adenine, N6-(4-aminobutyl)adenine, N6-(4-amino-trans-but-2-enyl)adenine, N6-(4-amino-cis-but-2-enyl)adenine and N6-(4-aminobut-2-ynyl)adenine. From these, N 6-(4-aminobutyl)adenine and N6-(4-amino-trans-but-2-enyl)adenine were found to be substrates for all three enzymes (Km similar to 10-4 M).Absorption spectroscopy demonstrated such an interaction with the cofactor topaquinone, which is typical for commondiamine substrates. However, only the former compound provided a regular reaction stoichiometry. Anaerobic absorptionspectra of N6-(3-aminopropyl)adenine, N6-(4-amino-cis-but-2-enyl)adenine and N6
- Plant copper/topaquinone-containing amine oxidases (CAOs, EC 1.4.3.6) are enzymes oxidising various amines. Here wereport a study on the reactions of CAOs from grass pea (Lathyrus sativus), lentil (Lens esculenta) and Euphorbia characias, aMediterranean shrub, with N6-aminoalkyl adenines representing combined analogues of cytokinins and polyamines. Thefollowing compounds were synthesised: N6-(3-aminopropyl)adenine, N6-(4-aminobutyl)adenine, N6-(4-amino-trans-but-2-enyl)adenine, N6-(4-amino-cis-but-2-enyl)adenine and N6-(4-aminobut-2-ynyl)adenine. From these, N 6-(4-aminobutyl)adenine and N6-(4-amino-trans-but-2-enyl)adenine were found to be substrates for all three enzymes (Km similar to 10-4 M).Absorption spectroscopy demonstrated such an interaction with the cofactor topaquinone, which is typical for commondiamine substrates. However, only the former compound provided a regular reaction stoichiometry. Anaerobic absorptionspectra of N6-(3-aminopropyl)adenine, N6-(4-amino-cis-but-2-enyl)adenine and N6 (en)
- Několik N6-alkylaminoadeninů bylo připraveno jako trihydrochloridy v dostatečné čistotě pro další studia - ABAD, N6-(4-aminobutyl)adenin, ABYAD, N6-(4-aminobut-2-ynyl)adenin, ACBAD, N6-(4-amino-cis-but-2-enyl)adenin, APAD, N6-(3- aminopropyl)-adenin a ATBAD, N6-(4-amino-trans-but-2-enyl)adenin. Tyto látky byly plně charakterizovány a testovány s enzymy GPAO - aminoxidasa z hrachoru setého, ELAO - aminoxidasa z latexu Euphorbia characias, LSAO - aminoxidasa z čočky. Přítomnost adeninového cyklu nebrání interakci s enzymem. ABAD i ATBAD mohou být považovány za substráty. Hodnoty Km těchto látek se blíží hodnotám známým pro subtrát putrescin, ačkoli látky jeví odlišnou stechiometrii reakce. APAD a ACBAD se projevují jako reverzibilní inhibitory, čímž se ACBAD liší od svého geometrického izomeru ATBAD. ABYAD je reakčně závislým inhibitorem zkoumaných aminoxidas. Experimenty s rychlým zaznamenáváním spekter ukázaly, že ACBAD a APAD netvoří při enyzmové přeměně meziprodukty typické pro substráty aminoxidas (cs)
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| Title
| - Reactions of plant copper/topaquinone amine oxidases with N6-aminoalkyl derivatives of adenine
- Reactions of plant copper/topaquinone amine oxidases with N6-aminoalkyl derivatives of adenine (en)
- Reakce rostlinných Cu-aminoxidas s N6-aminoalkylderiváty adeninu (cs)
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| skos:prefLabel
| - Reactions of plant copper/topaquinone amine oxidases with N6-aminoalkyl derivatives of adenine
- Reactions of plant copper/topaquinone amine oxidases with N6-aminoalkyl derivatives of adenine (en)
- Reakce rostlinných Cu-aminoxidas s N6-aminoalkylderiváty adeninu (cs)
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| skos:notation
| - RIV/61989592:15310/05:00002183!RIV06-MSM-15310___
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| http://linked.open.../vavai/riv/strany
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
| - Z(MSM 153100010), Z(MSM 153100013)
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
| |
| http://linked.open...ai/riv/idVysledku
| - RIV/61989592:15310/05:00002183
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| http://linked.open...riv/jazykVysledku
| |
| http://linked.open.../riv/klicovaSlova
| - CYCLIN-DEPENDENT KINASES; PEA DIAMINE OXIDASE; CYTOKININ OXIDASE; MECHANISM; INHIBITION; OLOMOUCINE; SEEDLINGS; DEHYDROGENASE; INACTIVATION; PURIFICATION (en)
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| http://linked.open.../riv/klicoveSlovo
| |
| http://linked.open...odStatuVydavatele
| - GB - Spojené království Velké Británie a Severního Irska
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| http://linked.open...ontrolniKodProRIV
| |
| http://linked.open...i/riv/nazevZdroje
| - Journal of Enzyme Inhibition and Medicinal Chemistry
|
| http://linked.open...in/vavai/riv/obor
| |
| http://linked.open...ichTvurcuVysledku
| |
| http://linked.open...cetTvurcuVysledku
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| http://linked.open...UplatneniVysledku
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| http://linked.open...v/svazekPeriodika
| |
| http://linked.open...iv/tvurceVysledku
| - Šebela, Marek
- Fryčák, Petr
- Floris, Giovanni
- Medda, Rosaria
- Peč, Pavel
- Lamplot, Zbyněk
- Longu, Silvia
- Padiglia, Alessandra
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| http://linked.open...n/vavai/riv/zamer
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| issn
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| number of pages
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| http://localhost/t...ganizacniJednotka
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