| Attributes | Values |
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| rdf:type
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| Description
| - Enzym cytokinin oxidasa/dehydrogenasa (CKO/CKX) z kukuřice byl připraven jako rekombinantní protein v hostitelské kvasince Yarrowia lipolytica. Rekombinantní enzym byl izolován z kultury transformovaných kvasinek a přečištěn do homogenního stavu. Následně byly charakterizovány molekulové a kinetické vlastnosti enzymu. (cs)
- Cytokinin oxidase/dehydrogenase (CKO/CKX) is a flavoenzyme, which irreversibly inactivates cytokinins by severing the isoprenoid side chain from the adenine/adenosine moiety. There are several genes coding for the enzyme in maize (Zea mays). A Z. mays CKO1 cDNA was cloned in the yeast Yarrowia lipolytica to achieve heterologous protein expression. The recombinant ZmCKO1 was recovered from cultures of transformed yeasts and purified using several chromatographic steps. The enzyme was obtained as a homogeneous protein in a remarkably high-yield and its molecular and kinetic properties were characterized. The enzyme showed a molecular mass of 69 kDa, pI was 6.3. Neutral sugar content of the molecule was 22%. Absorption and fluorescence spectra were in accordance with the presence of FAD as a cofactor. Peptide mass fingerprinting using MALDI-MS correctly assigned the enzyme in MSDB protein database. The enzyme showed a relatively high degree of thermostability (T50 = 55 °C for 30 min incubation). The foll
- Cytokinin oxidase/dehydrogenase (CKO/CKX) is a flavoenzyme, which irreversibly inactivates cytokinins by severing the isoprenoid side chain from the adenine/adenosine moiety. There are several genes coding for the enzyme in maize (Zea mays). A Z. mays CKO1 cDNA was cloned in the yeast Yarrowia lipolytica to achieve heterologous protein expression. The recombinant ZmCKO1 was recovered from cultures of transformed yeasts and purified using several chromatographic steps. The enzyme was obtained as a homogeneous protein in a remarkably high-yield and its molecular and kinetic properties were characterized. The enzyme showed a molecular mass of 69 kDa, pI was 6.3. Neutral sugar content of the molecule was 22%. Absorption and fluorescence spectra were in accordance with the presence of FAD as a cofactor. Peptide mass fingerprinting using MALDI-MS correctly assigned the enzyme in MSDB protein database. The enzyme showed a relatively high degree of thermostability (T50 = 55 °C for 30 min incubation). The foll (en)
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| Title
| - High-level expression and characterization of Zea mays cytokinin oxidase/dehydrogenase in Yarrowia lipolytica
- Vysokoúrovňová exprese a charakterizace cytokininoxidasy/dehydrogenasy ze Zea mays v Yarrowia lipolytica (cs)
- High-level expression and characterization of Zea mays cytokinin oxidase/dehydrogenase in Yarrowia lipolytica (en)
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| skos:prefLabel
| - High-level expression and characterization of Zea mays cytokinin oxidase/dehydrogenase in Yarrowia lipolytica
- Vysokoúrovňová exprese a charakterizace cytokininoxidasy/dehydrogenasy ze Zea mays v Yarrowia lipolytica (cs)
- High-level expression and characterization of Zea mays cytokinin oxidase/dehydrogenase in Yarrowia lipolytica (en)
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| skos:notation
| - RIV/61989592:15310/05:00002162!RIV06-MSM-15310___
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| http://linked.open.../vavai/riv/strany
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
| - P(ME 664), Z(MSM6198959216)
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/61989592:15310/05:00002162
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - POLYACRYLAMIDE-GELS; POLYAMINE OXIDASE; YEAST; DEHYDROGENASE; PURIFICATION; PROTEINS; DEGRADATION; CLONING; MAIZE; ACID (en)
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| http://linked.open.../riv/klicoveSlovo
| |
| http://linked.open...odStatuVydavatele
| - FR - Francouzská republika
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
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| http://linked.open...in/vavai/riv/obor
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| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...vavai/riv/projekt
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| http://linked.open...UplatneniVysledku
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| http://linked.open...v/svazekPeriodika
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| http://linked.open...iv/tvurceVysledku
| - Kopečný, David
- Šebela, Marek
- Madzak, Catherine
- Houba-Hérin, Nicole
- Laloue, Michel
- Majira, Amel
- Pethe, Claude
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| http://linked.open...n/vavai/riv/zamer
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| issn
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| number of pages
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| http://localhost/t...ganizacniJednotka
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