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| rdf:type
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| Description
| - Cytokinin dehydrogenase (CKX) is a flavoprotein that cleaves cytokinins to adenine and corresponding side-chain aldehyde using a quinone-type electron acceptor. Reactions of maize CKX with five different substrates were studied using the Stopped-flow monitoring of the reductive half-reaction revealed formation of a binary ES complex between the formed cytokinin imine and the reduced enzyme. KD values for cytokinin binding range from <2 µM for isopentenyladenine to 66 µM for kinetin and reasonably correspond to respective Km values. Reduction rate was high for isoprenoid cytokinins that showed specific formation of a charge transfer complex with the enzyme, but very low for other cytokinins. The ES complex decays to form a free imine-product which accumulates in the reaction mixture and only slowly hydrolyzes to adenine and the corresponding aldehyde. The imine products were identified using Q-TOF MS/MS. Mixing of the substrate-reduced enzyme with Cu2+/imidazole as an electron acceptor, was used to
- Cytokinin dehydrogenase (CKX) is a flavoprotein that cleaves cytokinins to adenine and corresponding side-chain aldehyde using a quinone-type electron acceptor. Reactions of maize CKX with five different substrates were studied using the Stopped-flow monitoring of the reductive half-reaction revealed formation of a binary ES complex between the formed cytokinin imine and the reduced enzyme. KD values for cytokinin binding range from <2 µM for isopentenyladenine to 66 µM for kinetin and reasonably correspond to respective Km values. Reduction rate was high for isoprenoid cytokinins that showed specific formation of a charge transfer complex with the enzyme, but very low for other cytokinins. The ES complex decays to form a free imine-product which accumulates in the reaction mixture and only slowly hydrolyzes to adenine and the corresponding aldehyde. The imine products were identified using Q-TOF MS/MS. Mixing of the substrate-reduced enzyme with Cu2+/imidazole as an electron acceptor, was used to (en)
- Cytokinindehydrogenasa (CKX) je flavoprotein, který štěpí cytokininy na adenine a aldehyd z postranního řetězce. Jednotlivé faze reakce CKX z kukuřice byly zkoumány s různými substráty pomocí spektrofotometrie zastaveného toku a byly identifikovány struktury iminových meziproduktů pomocí Q-TOF MS/MS. Kinetická data byla diskutována ve vztahu ke struktuře proteinu. (cs)
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| Title
| - Kinetic analysis and structural correlations of the reaction of cytokinin dehydrogenase (EC. 1.5.99.12)
- Kinetic analysis and structural correlations of the reaction of cytokinin dehydrogenase (EC. 1.5.99.12) (en)
- Kinetická analýza a strukturní korelace reakce cytokinindehydrogenasy (cs)
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| skos:prefLabel
| - Kinetic analysis and structural correlations of the reaction of cytokinin dehydrogenase (EC. 1.5.99.12)
- Kinetic analysis and structural correlations of the reaction of cytokinin dehydrogenase (EC. 1.5.99.12) (en)
- Kinetická analýza a strukturní korelace reakce cytokinindehydrogenasy (cs)
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| skos:notation
| - RIV/61989592:15310/05:00001818!RIV06-MSM-15310___
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| http://linked.open.../vavai/riv/strany
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
| - P(GA522/03/0979), P(GP204/03/P103), Z(MSM6198959216)
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/61989592:15310/05:00001818
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - catalytic mechanism; crystal structure; cytokinin dehydrogenase; flavoprotein; imine (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...odStatuVydavatele
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
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| http://linked.open...in/vavai/riv/obor
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| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...vavai/riv/projekt
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| http://linked.open...UplatneniVysledku
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| http://linked.open...v/svazekPeriodika
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| http://linked.open...iv/tvurceVysledku
| - Novák, Ondřej
- Popelková, Hana
- Frébort, Ivo
- Frébortová, Jitka
- Bilyeu, Kristin D.
- Fraaije, Marco W.
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| http://linked.open...n/vavai/riv/zamer
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| issn
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| number of pages
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| http://localhost/t...ganizacniJednotka
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