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| Description
| - Background DNA flow cytometry describes the use of flow cytometry for estimation of DNA quantity in cell nuclei. The method involves preparation of aqueous suspensions of intact nuclei whose DNA is stained using a DNA fluorochrome. The nuclei are classified according to their relative fluorescence intensity or DNA content. Because the sample preparation and analysis is convenient and rapid, DNA flow cytometry, has become a popular method for ploidy screening, detection of mixoploidy and aneuploidy, cell cycle analysis, assessment of the degree of polysomaty. determination of reproductive pathway, and estimation of absolute DNA amount or genome size. While the former applications are relatively straightforward, estimation of absolute DNA amount require special attention to possible errors in sample preparation and analysis. Scope The article reviews current procedures for estimation of absolute DNA amounts in plants using flow cytometry, with special emphasis on preparation of nuclei suspensions, stoic
- Background DNA flow cytometry describes the use of flow cytometry for estimation of DNA quantity in cell nuclei. The method involves preparation of aqueous suspensions of intact nuclei whose DNA is stained using a DNA fluorochrome. The nuclei are classified according to their relative fluorescence intensity or DNA content. Because the sample preparation and analysis is convenient and rapid, DNA flow cytometry, has become a popular method for ploidy screening, detection of mixoploidy and aneuploidy, cell cycle analysis, assessment of the degree of polysomaty. determination of reproductive pathway, and estimation of absolute DNA amount or genome size. While the former applications are relatively straightforward, estimation of absolute DNA amount require special attention to possible errors in sample preparation and analysis. Scope The article reviews current procedures for estimation of absolute DNA amounts in plants using flow cytometry, with special emphasis on preparation of nuclei suspensions, stoic (en)
- Kvalitní stanovení absolutního obsahu DNA u rostlin za pomocí průtokové cytometrie není triviálním úkolem. Ačkoli existuje několik ověřených protokolů a některé faktory kontrolující přesnost a reprodukovatelnost byly identifikovány, stále zůstávají některé problémy: (1) nezbytnost čerstvé tkáně komplikuje transfer %22doma připravených%22 vzorků a/nebo jejich skladování: (2) úloha cytosolických složek interferujících s kvantitativním DNA barvením není příliš známa: a (3) užití sady 4 mezinárodně uznaných DNA referenčních standardů zůstává stále nerealizovaným cílem. (cs)
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| Title
| - Plant DNA flow cytometry and estimation of nuclear genome size L093/aob/inci005
- Rostlinná flow-cytometrie a stanovení velikosti jaderného genomu (cs)
- Plant DNA flow cytometry and estimation of nuclear genome size L093/aob/inci005 (en)
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| skos:prefLabel
| - Plant DNA flow cytometry and estimation of nuclear genome size L093/aob/inci005
- Rostlinná flow-cytometrie a stanovení velikosti jaderného genomu (cs)
- Plant DNA flow cytometry and estimation of nuclear genome size L093/aob/inci005 (en)
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| skos:notation
| - RIV/61989592:15310/05:00001811!RIV06-GA0-15310___
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| http://linked.open.../vavai/riv/strany
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
| - P(GA204/04/1207), P(GA522/03/0354)
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/61989592:15310/05:00001811
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - flow cytometry; nuclear genome size; DNA C-value; nuclear DNA amount; DNA staining; intraspecific variation PROPIDIUM IODIDE; BASE COMPOSITION; CELL-CYCLE; STOICHIOMETRIC ERROR; REFERENCE-STANDARDS; HORDEUM-SPONTANEUM; IMAGE CYTOMETRY; MATURITY GROUP; BINDING-SITES; SINGLE CELLS (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...odStatuVydavatele
| - GB - Spojené království Velké Británie a Severního Irska
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
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| http://linked.open...in/vavai/riv/obor
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| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...vavai/riv/projekt
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| http://linked.open...UplatneniVysledku
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| http://linked.open...v/svazekPeriodika
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| http://linked.open...iv/tvurceVysledku
| - Bartoš, Jan
- Doležel, Jaroslav
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| issn
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| number of pages
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| http://localhost/t...ganizacniJednotka
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