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  • The presence of phytoplasmas in the shrubs of red and white currants exhibiting full blossom symptoms was determined by nested PCR with primers amplifying phytoplasma 16S rRNA gene, sequencing and by electron microscopy. In total, 6 of 10 analysed symptomatic bushes gave positive PCR reaction. Based on the results of sequencing the phytoplasma isolates were identified as belonging to the apple proliferation group and aster yellows group. The presence of phytoplasma-like bodies in the phloem of two red currant bushes infected with aster yellows and apple proliferation phytoplasmas was confirmed by electron microscopy. All 10 analysed bushes were to be found infected with the Blackcurrant reversion virus. In graft experiments, the disease was transmitted from infected bushes to red currant ´Rondom´, ´Jonkheer van Tets´ and ´Vitan´, white currant ´Blanka´ and black currant ´Viola´. Red and white currants showed symptoms of full blossom, whereas ´Viola´ symptoms of the severe form of reversion.
  • The presence of phytoplasmas in the shrubs of red and white currants exhibiting full blossom symptoms was determined by nested PCR with primers amplifying phytoplasma 16S rRNA gene, sequencing and by electron microscopy. In total, 6 of 10 analysed symptomatic bushes gave positive PCR reaction. Based on the results of sequencing the phytoplasma isolates were identified as belonging to the apple proliferation group and aster yellows group. The presence of phytoplasma-like bodies in the phloem of two red currant bushes infected with aster yellows and apple proliferation phytoplasmas was confirmed by electron microscopy. All 10 analysed bushes were to be found infected with the Blackcurrant reversion virus. In graft experiments, the disease was transmitted from infected bushes to red currant ´Rondom´, ´Jonkheer van Tets´ and ´Vitan´, white currant ´Blanka´ and black currant ´Viola´. Red and white currants showed symptoms of full blossom, whereas ´Viola´ symptoms of the severe form of reversion. (en)
  • Pomocí PCR, sekvenování a elektronové mikroskopie byla sledována přítomnost fytoplazem v keřích červeného a bílého rybízu vykazujících plnokvětost. Na základě sekvenování byly detekováno fytoplazmy určeny jako fytoplazma žloutenky aster a fytoplazma proliferace jabloní. Tělískap odobná fytoplazmám byla identifikována I pomocí elektronové mikroskopie. U všech deset testovaných keřů byla detekována přítomnost viru zvratu rybízu. V roubovacích experimentech byl potvrzen přenos choroby na zdravé rostliny. (cs)
Title
  • Present Status of Research on the Etiology of the Full Blossom Disease in Red and White Currants in the Czech Republic
  • Present Status of Research on the Etiology of the Full Blossom Disease in Red and White Currants in the Czech Republic (en)
  • Současný stav výzkumu etiologie plnokvětosti u červeného a bílého rybízu v České republice. (cs)
skos:prefLabel
  • Present Status of Research on the Etiology of the Full Blossom Disease in Red and White Currants in the Czech Republic
  • Present Status of Research on the Etiology of the Full Blossom Disease in Red and White Currants in the Czech Republic (en)
  • Současný stav výzkumu etiologie plnokvětosti u červeného a bílého rybízu v České republice. (cs)
skos:notation
  • RIV/61989592:15310/04:00002450!RIV/2005/GA0/153105/N
http://linked.open.../vavai/riv/strany
  • 99-102
http://linked.open...avai/riv/aktivita
http://linked.open...avai/riv/aktivity
  • P(GA522/02/0040)
http://linked.open...iv/cisloPeriodika
  • -
http://linked.open...vai/riv/dodaniDat
http://linked.open...aciTvurceVysledku
http://linked.open.../riv/druhVysledku
http://linked.open...iv/duvernostUdaju
http://linked.open...titaPredkladatele
http://linked.open...dnocenehoVysledku
  • 581597
http://linked.open...ai/riv/idVysledku
  • RIV/61989592:15310/04:00002450
http://linked.open...riv/jazykVysledku
http://linked.open.../riv/klicovaSlova
  • phytoplasma;apple proliferation;aster yellows;Blackcurrant reversion virus;PCR;16S rRNA;red and white currant;sequencing;electron microscopy; (en)
http://linked.open.../riv/klicoveSlovo
http://linked.open...odStatuVydavatele
  • NL - Nizozemsko
http://linked.open...ontrolniKodProRIV
  • [A58EA2DB0595]
http://linked.open...i/riv/nazevZdroje
  • Acta Horticulturae
http://linked.open...in/vavai/riv/obor
http://linked.open...ichTvurcuVysledku
http://linked.open...cetTvurcuVysledku
http://linked.open...vavai/riv/projekt
http://linked.open...UplatneniVysledku
http://linked.open...v/svazekPeriodika
  • 656
http://linked.open...iv/tvurceVysledku
  • Navrátil, Milan
  • Válová, Pavla
  • Fialová, Renata
  • Špak, J.
  • Karešová, R.
  • Přibylová, J.
  • Kubelková, D.
  • Špaková, V.
issn
  • 0567-7572
number of pages
http://localhost/t...ganizacniJednotka
  • 15310
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