About: Adaptation of enzyme-linked immunosorbent assay (ELISA) to the determination of brassinosteroids in plant tissues

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About: Adaptation of enzyme-linked immunosorbent assay (ELISA) to the determination of brassinosteroids in plant tissues Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Byla studována adaptace enzymové imunoanalýzy pro stanovení brassinosteroidů v rostlinném materiálu. (cs) Brassinosteroids (BRs) represent a large group of plant steroids which include more than 40 congeners distributed from lower to higher plants. BRs have been detected and isolated from seeds, fruits, leaves, galls and pollen. Physiological functions proposed for BRs include cell elongation, cell division and modulation of stress responses.We have developed polyclonal antibodies against a brassinosteroid analogue, (20S)-2?,3?-dihydroxy-7-oxa-7?-homo-5?-pregnane-6one-20 carboxylic acid (4812). Antiserum against this substance was raised in rabbits immunized using 4812 - bovine-serum albumin (BSA) conjugate. The conjugate was prepared by mixed anhydride method.Polyclonal antibodies raised in rabbits were purified by ammonium sulphate precipitation or by affinity purification on protein A columns. The obtained antibodies were tested in enzyme-linked immunosorbent assay (ELISA) using (20S)-2?,3?-dihydroxy-7-oxa-7?-homo-5?-pregnane-6one-20 carboxylic acid - horse-radish peroxidase conjugate.The cross Brassinosteroids (BRs) represent a large group of plant steroids which include more than 40 congeners distributed from lower to higher plants. BRs have been detected and isolated from seeds, fruits, leaves, galls and pollen. Physiological functions proposed for BRs include cell elongation, cell division and modulation of stress responses.We have developed polyclonal antibodies against a brassinosteroid analogue, (20S)-2?,3?-dihydroxy-7-oxa-7?-homo-5?-pregnane-6one-20 carboxylic acid (4812). Antiserum against this substance was raised in rabbits immunized using 4812 - bovine-serum albumin (BSA) conjugate. The conjugate was prepared by mixed anhydride method.Polyclonal antibodies raised in rabbits were purified by ammonium sulphate precipitation or by affinity purification on protein A columns. The obtained antibodies were tested in enzyme-linked immunosorbent assay (ELISA) using (20S)-2?,3?-dihydroxy-7-oxa-7?-homo-5?-pregnane-6one-20 carboxylic acid - horse-radish peroxidase conjugate.The cross (en)
Title	Adaptation of enzyme-linked immunosorbent assay (ELISA) to the determination of brassinosteroids in plant tissues Adaptation of enzyme-linked immunosorbent assay (ELISA) to the determination of brassinosteroids in plant tissues (en) Adaptace enzymové imunoanalýzy pro stanovení brassinosteroidů v rostlinném materiálu (cs)
skos:prefLabel	Adaptation of enzyme-linked immunosorbent assay (ELISA) to the determination of brassinosteroids in plant tissues Adaptation of enzyme-linked immunosorbent assay (ELISA) to the determination of brassinosteroids in plant tissues (en) Adaptace enzymové imunoanalýzy pro stanovení brassinosteroidů v rostlinném materiálu (cs)
skos:notation	RIV/61989592:15310/04:00002318!RIV/2005/AV0/153105/N
http://linked.open.../vavai/riv/strany	28
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(IBS5038351)
http://linked.open...iv/cisloPeriodika	-
http://linked.open...vai/riv/dodaniDat	2005
http://linked.open...aciTvurceVysledku	Strnad, Miroslav Oklešťková, Jana
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Palackého v Olomouci / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	553506
http://linked.open...ai/riv/idVysledku	RIV/61989592:15310/04:00002318
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	brassinosteroids;polyclonal antibodies;ELISA (en)
http://linked.open.../riv/klicoveSlovo	ELISA brassinosteroids polyclonal antibodies
http://linked.open...odStatuVydavatele	PL - Polská republika
http://linked.open...ontrolniKodProRIV	[4F8E42086ECE]
http://linked.open...i/riv/nazevZdroje	Acta Physiologiae Plantarum
http://linked.open...in/vavai/riv/obor	EF
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...vavai/riv/projekt	Immunodiagnostics of natural hormonal substances
http://linked.open...UplatneniVysledku	2004
http://linked.open...v/svazekPeriodika	26
http://linked.open...iv/tvurceVysledku	Kohout, Ladislav Strnad, Miroslav Swaczynová, Jana
issn	0137-5881
number of pages	318 (xsd:int)
http://localhost/t...ganizacniJednotka	15310

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