About: Cytokinin oxidase/dehydrogenase genes in barley and wheat

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Práce je zaměřena na geny kodující cytokinin oxydasu/dehydrogenasu v ječmeni a pšenici. (cs) The cloning of two novel genes that encode cytokinin oxidase/dehydrogenase (CKX) in barley is described in this work. Transformation of both genes into Arabidopsis and tobacco showed that at least one of the genes codes for a functional enzyme, as its expression caused a cytokinin-deficient phenotype in the heterologous host plants. Additional cloning of two gene fragments, and an in silico search in the public expressed sequence tag clone databases, revealed the presence of at least 13 more members of the CKX gene family in barley and wheat. The expression of three selected barley genes was analyzed by RT-PCR and found to be organ-specific with peak expression in mature kernels. One barley CKX (HvCKX2) was characterized in detail after heterologous expression in tobacco. Interestingly, this enzyme shows a pH optimum at 4.5 and a preference for cytokinin ribosides as substrates, which may indicate its vacuolar targeting. Different substrate specificities, and the pH profiles of cytokinin-degrading enz The cloning of two novel genes that encode cytokinin oxidase/dehydrogenase (CKX) in barley is described in this work. Transformation of both genes into Arabidopsis and tobacco showed that at least one of the genes codes for a functional enzyme, as its expression caused a cytokinin-deficient phenotype in the heterologous host plants. Additional cloning of two gene fragments, and an in silico search in the public expressed sequence tag clone databases, revealed the presence of at least 13 more members of the CKX gene family in barley and wheat. The expression of three selected barley genes was analyzed by RT-PCR and found to be organ-specific with peak expression in mature kernels. One barley CKX (HvCKX2) was characterized in detail after heterologous expression in tobacco. Interestingly, this enzyme shows a pH optimum at 4.5 and a preference for cytokinin ribosides as substrates, which may indicate its vacuolar targeting. Different substrate specificities, and the pH profiles of cytokinin-degrading enz (en)
Title	Cytokinin oxidase/dehydrogenase genes in barley and wheat Cytokinin oxidase/dehydrogenase genes in barley and wheat (en) Geny kodující cytokinin oxydasu/dehydrogenasu v ječmeni a pšenici (cs)
skos:prefLabel	Cytokinin oxidase/dehydrogenase genes in barley and wheat Cytokinin oxidase/dehydrogenase genes in barley and wheat (en) Geny kodující cytokinin oxydasu/dehydrogenasu v ječmeni a pšenici (cs)
skos:notation	RIV/61989592:15310/04:00002311!RIV/2005/GA0/153105/N
http://linked.open.../vavai/riv/strany	3999-4002
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(GA301/02/0475), P(IBS5038351)
http://linked.open...iv/cisloPeriodika	20
http://linked.open...vai/riv/dodaniDat	2005
http://linked.open...aciTvurceVysledku	Strnad, Miroslav Frébort, Ivo Frébortová, Jitka Galuszka, Petr
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Palackého v Olomouci / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	559207
http://linked.open...ai/riv/idVysledku	RIV/61989592:15310/04:00002311
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	cereals;cloning;cytokinin oxidase/dehydrogenase;expression;gene family (en)
http://linked.open.../riv/klicoveSlovo	cereals expression cytokinin oxidase/dehydrogenase gene family cloning
http://linked.open...odStatuVydavatele	DE - Spolková republika Německo
http://linked.open...ontrolniKodProRIV	[C079F3ACCCDE]
http://linked.open...i/riv/nazevZdroje	European Journal of Biochemistry
http://linked.open...in/vavai/riv/obor	EF
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	7 (xsd:int)
http://linked.open...vavai/riv/projekt	Purine inhibitors of cyclin-dependent kinases as anticancer and antimitotic compounds Immunodiagnostics of natural hormonal substances
http://linked.open...UplatneniVysledku	2004
http://linked.open...v/svazekPeriodika	271
http://linked.open...iv/tvurceVysledku	Strnad, Miroslav Frébort, Ivo Frébortová, Jitka Galuszka, Petr Werner, Tomáš Schmülling, Thomas Yamada, Mamoru
issn	0014-2956
number of pages	4 (xsd:int)
http://localhost/t...ganizacniJednotka	15310

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