About: Application of Q-TOF technology in plant hormone research

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An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Combination of high performance liquid chromatography (HPLC) or capillary liquid chromatography (CapLC) with quadrupole-time of flight mass spectrometry (Q-TOF) makes possible a new direction in plant hormone research. The instrument facilities are a good alternative for search of new plant metabolites and metabolic pathways. The recently developed method for quantitative analysis of natural cytokinins (Novák et al., 2003) was adapted from single-quadrupole mass spectrometer (SQ-MS) to Q-TOF micro instrument. The full scan spectra at very low concentration levels (100 fmol) were obtained together with MS-MS analyses of selected ions. The accurate masses of the parent ions and their fragments were calculated and used for determination of elementary structure with fidelity 10 ppm and better with a external calibration. The optimal conditions for combination of HPLC and CapLC with Q-TOF, i.e. capillary and cone voltage, desolvation gas flow and temperature for effective ionization and collision energy fo Combination of high performance liquid chromatography (HPLC) or capillary liquid chromatography (CapLC) with quadrupole-time of flight mass spectrometry (Q-TOF) makes possible a new direction in plant hormone research. The instrument facilities are a good alternative for search of new plant metabolites and metabolic pathways. The recently developed method for quantitative analysis of natural cytokinins (Novák et al., 2003) was adapted from single-quadrupole mass spectrometer (SQ-MS) to Q-TOF micro instrument. The full scan spectra at very low concentration levels (100 fmol) were obtained together with MS-MS analyses of selected ions. The accurate masses of the parent ions and their fragments were calculated and used for determination of elementary structure with fidelity 10 ppm and better with a external calibration. The optimal conditions for combination of HPLC and CapLC with Q-TOF, i.e. capillary and cone voltage, desolvation gas flow and temperature for effective ionization and collision energy fo (en) Kombinace HPLC nebo kapilární kapalinové chromatografie (CapLC) s Q-TOF dává nové možnosti ve výzkumu rostlinných hormonů. (cs)
Title	Application of Q-TOF technology in plant hormone research Application of Q-TOF technology in plant hormone research (en) Použití Q-TOF technologie při studiu rostlinných hormonů (cs)
skos:prefLabel	Application of Q-TOF technology in plant hormone research Application of Q-TOF technology in plant hormone research (en) Použití Q-TOF technologie při studiu rostlinných hormonů (cs)
skos:notation	RIV/61989592:15310/04:00002217!RIV/2005/GA0/153105/N
http://linked.open.../vavai/riv/strany	124
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(GA522/03/0323), Z(MSM 153100008)
http://linked.open...vai/riv/dodaniDat	2005
http://linked.open...aciTvurceVysledku	Novák, Ondřej Strnad, Miroslav Lenobel, René
http://linked.open.../riv/druhVysledku	D - Článek ve sborníku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Palackého v Olomouci / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	555158
http://linked.open...ai/riv/idVysledku	RIV/61989592:15310/04:00002217
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	liquid chromatography;mass spectrometry;quadrupole-time of flight mass spectrometer;cytokinins;identification;quantification;exact mass determination (en)
http://linked.open.../riv/klicoveSlovo	identification liquid chromatography mass spectrometry quantification cytokinins exact mass determination quadrupole-time of flight mass spectrometer
http://linked.open...ontrolniKodProRIV	[84106E3138A2]
http://linked.open...i/riv/mistoVydani	Canberra
http://linked.open...i/riv/nazevZdroje	18th IPGSA conference, Conference Handbook
http://linked.open...in/vavai/riv/obor	EF
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...vavai/riv/projekt	Investigation of algae and cyanobacteria as producers of biologically active substances
http://linked.open...UplatneniVysledku	2004
http://linked.open...iv/tvurceVysledku	Novák, Ondřej Strnad, Miroslav Lenobel, René Richer, Stephanie
http://linked.open...n/vavai/riv/zamer	http://linked.opendata.cz/resource/domain/vavai/zamer/MSM%20153100008
number of pages	144 (xsd:int)
http://purl.org/ne...btex#hasPublisher	Australian National University
http://localhost/t...ganizacniJednotka	15310

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