| Attributes | Values |
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| rdf:type
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| Description
| - Cytokinin dehydrogenase (CKX, EC 1.5.99.12) controls cytokinin levels in plants by oxidative cleavage of their side chain. The amount of cytokinin dehydrogenase in plants is generally very low and its isolation and purification is thus challenging. The cloning of CKX gene from maize1,2 opened a new opportunity for study of CKX and cytokinin function in plants. In Arabidopsis thaliana, seven distinct genes coding for seven proteins (AtCKX1 to AtCKX7) were found. In present study we aim to prepare yeast strains for production of recombinant AtCKX2 and to characterize CKX enzymes in tobacco plants overexpressing individual enzymes from Arabidopsis thaliana. AtCKX2 gene was cloned into pYES2 and pDR197 yeast expression plasmids or integrated into yeast genome using vectors YIplac211 and pYESX. The Saccharomyces cerevisiae strain 23344c (MAT#Á ura3) was used. CKX was secreted into the media in all tested yeast systems, the constitutive and inducible promoter driven expression showed comparable enzymatic ac
- Cytokinin dehydrogenase (CKX, EC 1.5.99.12) controls cytokinin levels in plants by oxidative cleavage of their side chain. The amount of cytokinin dehydrogenase in plants is generally very low and its isolation and purification is thus challenging. The cloning of CKX gene from maize1,2 opened a new opportunity for study of CKX and cytokinin function in plants. In Arabidopsis thaliana, seven distinct genes coding for seven proteins (AtCKX1 to AtCKX7) were found. In present study we aim to prepare yeast strains for production of recombinant AtCKX2 and to characterize CKX enzymes in tobacco plants overexpressing individual enzymes from Arabidopsis thaliana. AtCKX2 gene was cloned into pYES2 and pDR197 yeast expression plasmids or integrated into yeast genome using vectors YIplac211 and pYESX. The Saccharomyces cerevisiae strain 23344c (MAT#Á ura3) was used. CKX was secreted into the media in all tested yeast systems, the constitutive and inducible promoter driven expression showed comparable enzymatic ac (en)
- Článek se zabývá charakterizací cytokinindehydrogenasy z Arabidopsis thaliana exprimované v kvasince Saccharomyces cerevisiae a rostlinách tabáku. (cs)
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| Title
| - Characterization of Arabidopsis cytokinin dehydrogenase expressed in yeast Saccharomyces cerevisiae and tobacco plants
- Characterization of Arabidopsis cytokinin dehydrogenase expressed in yeast Saccharomyces cerevisiae and tobacco plants (en)
- Charakterizace cytokinindehydrogenasy z Arabidopsis thaliana exprimované v kvasince Saccharomyces cerevisiae a rostlinách tabáku (cs)
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| skos:prefLabel
| - Characterization of Arabidopsis cytokinin dehydrogenase expressed in yeast Saccharomyces cerevisiae and tobacco plants
- Characterization of Arabidopsis cytokinin dehydrogenase expressed in yeast Saccharomyces cerevisiae and tobacco plants (en)
- Charakterizace cytokinindehydrogenasy z Arabidopsis thaliana exprimované v kvasince Saccharomyces cerevisiae a rostlinách tabáku (cs)
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| skos:notation
| - RIV/61989592:15310/04:00002103!RIV/2005/GA0/153105/N
|
| http://linked.open.../vavai/riv/strany
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
| - P(GA522/03/0979), Z(MSM 153100008)
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| http://linked.open...iv/cisloPeriodika
| |
| http://linked.open...vai/riv/dodaniDat
| |
| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
| |
| http://linked.open...ai/riv/idVysledku
| - RIV/61989592:15310/04:00002103
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| http://linked.open...riv/jazykVysledku
| |
| http://linked.open.../riv/klicovaSlova
| - cytokinin oxidase/dehydrogenase;cloning;expression (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...odStatuVydavatele
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
| - Acta Universitatis Palackianae Olomucensis, Facultas Rerum Naturalium, Chemica
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| http://linked.open...in/vavai/riv/obor
| |
| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
| |
| http://linked.open...vavai/riv/projekt
| |
| http://linked.open...UplatneniVysledku
| |
| http://linked.open...v/svazekPeriodika
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| http://linked.open...iv/tvurceVysledku
| - Drábek, Jiří
- Popelková, Hana
- Frébort, Ivo
- Frébortová, Jitka
- Galuszka, Petr
- Werner, Tomáš
- Schmuelling, Thomas
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| http://linked.open...n/vavai/riv/zamer
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| issn
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| number of pages
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| http://localhost/t...ganizacniJednotka
| |
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