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Description
| - We present the structure of LinB, a 33-kDa haloalkane dehalogenase from Sphingomonas paucimobilis UT26, at 0.95 Angstrom resolution. The data have allowed us to directly observe the anisotropic motions of the catalytic residues. In particular, the side-chain of the catalytic nucleophile, Asp 108, displays a high degree of disorder. It has been modeled in two conformations, one similar to that observed previously (conformation A) and one strained (conformation 13) that approached the catalytic base (His272). The strain in conformation B was mainly in the C-alpha-C-beta-C-gamma angle (126degrees) that deviated by 13.4degrees from the %22ideal%22 bond angle of 112.6degrees. On the basis of these observations, we propose a role for the charge state of the catalytic histidine in determining the geometry of the catalytic residues. We hypothesized that double-protonation of the catalytic base (His272) reduces the distance between the side-chain of this residue and that of the Asp108. The results of molecular dyn
- We present the structure of LinB, a 33-kDa haloalkane dehalogenase from Sphingomonas paucimobilis UT26, at 0.95 Angstrom resolution. The data have allowed us to directly observe the anisotropic motions of the catalytic residues. In particular, the side-chain of the catalytic nucleophile, Asp 108, displays a high degree of disorder. It has been modeled in two conformations, one similar to that observed previously (conformation A) and one strained (conformation 13) that approached the catalytic base (His272). The strain in conformation B was mainly in the C-alpha-C-beta-C-gamma angle (126degrees) that deviated by 13.4degrees from the %22ideal%22 bond angle of 112.6degrees. On the basis of these observations, we propose a role for the charge state of the catalytic histidine in determining the geometry of the catalytic residues. We hypothesized that double-protonation of the catalytic base (His272) reduces the distance between the side-chain of this residue and that of the Asp108. The results of molecular dyn (en)
- Prezentujeme krystalovou strukturu haloalkan dehalogenasy LinB z Sphingomonas paucimobilis UT26 v 0.95 angstrom rozlišení. (cs)
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Title
| - Crystal structure of haloalkane dehalogenase LinB from Sphingomonas paucimobilis UT26 at 0.95 angstrom resolution: Dynamics of catalytic residues
- Crystal structure of haloalkane dehalogenase LinB from Sphingomonas paucimobilis UT26 at 0.95 angstrom resolution: Dynamics of catalytic residues (en)
- Krystalová struktura haloalkan dehalogenasy LinB z Sphingomonas paucimobilis UT26 v 0.95 angstrom rozlišení (cs)
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skos:prefLabel
| - Crystal structure of haloalkane dehalogenase LinB from Sphingomonas paucimobilis UT26 at 0.95 angstrom resolution: Dynamics of catalytic residues
- Crystal structure of haloalkane dehalogenase LinB from Sphingomonas paucimobilis UT26 at 0.95 angstrom resolution: Dynamics of catalytic residues (en)
- Krystalová struktura haloalkan dehalogenasy LinB z Sphingomonas paucimobilis UT26 v 0.95 angstrom rozlišení (cs)
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skos:notation
| - RIV/61989592:15310/04:00001936!RIV/2005/MSM/153105/N
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http://linked.open.../vavai/riv/strany
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http://linked.open...avai/riv/aktivita
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http://linked.open...avai/riv/aktivity
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http://linked.open...iv/cisloPeriodika
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http://linked.open...vai/riv/dodaniDat
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http://linked.open...aciTvurceVysledku
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http://linked.open.../riv/druhVysledku
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http://linked.open...iv/duvernostUdaju
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http://linked.open...titaPredkladatele
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http://linked.open...dnocenehoVysledku
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http://linked.open...ai/riv/idVysledku
| - RIV/61989592:15310/04:00001936
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http://linked.open...riv/jazykVysledku
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http://linked.open.../riv/klicovaSlova
| - HEXACHLOROCYCLOHEXANE-DEGRADING BACTERIUM;PROTEIN-STRUCTURE REFINEMENT;HALIDE-BINDING-SITE;REACTION-MECHANISM;SUBSTRATE CLASS;SPECIFICITY;ENZYMES;PROGRAM;MUTAGENESIS;PARAMETERS (en)
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http://linked.open.../riv/klicoveSlovo
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http://linked.open...odStatuVydavatele
| - US - Spojené státy americké
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http://linked.open...ontrolniKodProRIV
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http://linked.open...i/riv/nazevZdroje
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http://linked.open...in/vavai/riv/obor
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http://linked.open...ichTvurcuVysledku
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http://linked.open...cetTvurcuVysledku
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http://linked.open...UplatneniVysledku
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http://linked.open...v/svazekPeriodika
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http://linked.open...iv/tvurceVysledku
| - Damborský, Jiří
- Klvaňa, Martin
- Otyepka, Michal
- Nagata, Yugi
- Oakley, A. J.
- Wilce, Mathew
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http://linked.open...n/vavai/riv/zamer
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issn
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number of pages
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http://localhost/t...ganizacniJednotka
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