About: Capillary electrophoresis for detection of inherited disorders of purine and pyrimidine metabolism: A selective approach

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About: Capillary electrophoresis for detection of inherited disorders of purine and pyrimidine metabolism: A selective approach Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	We developed a capillary electrophoresis method as a diagnostic tool for purine and pyrimidine metabolic disorders. Optimal conditions allowed the separation of the major diagnostic metabolites in urine samples within an analysis time of 10 min and with a separation efficiency of about 350 000 theoretical plates/m. The diagnostically important metabolites (adenine, adenosine, 2-deoxyadenosine, 2-deoxyguanosine, 2,8 dihydroxyadenine, guanosine, hypoxanthine, orotidine, orotic acid, and creatinine) were detectable at concentrations of 1.0 5.7 mumol/L. The method gives a linear calibration curve for tested purine and pyrimidine derivatives within the range of 5 500 mumol/L (r > 0.996) The coefficients of variation for the within- and between-day imprecisions were less than 3.2 and 5.8%, respectively. Characteristic abnormalities were detected in the electropherograms of urine samples from patients with purine and pyrimidine enzyme deficiencies. We provide electrophoretic and spectral characteristics of i We developed a capillary electrophoresis method as a diagnostic tool for purine and pyrimidine metabolic disorders. Optimal conditions allowed the separation of the major diagnostic metabolites in urine samples within an analysis time of 10 min and with a separation efficiency of about 350 000 theoretical plates/m. The diagnostically important metabolites (adenine, adenosine, 2-deoxyadenosine, 2-deoxyguanosine, 2,8 dihydroxyadenine, guanosine, hypoxanthine, orotidine, orotic acid, and creatinine) were detectable at concentrations of 1.0 5.7 mumol/L. The method gives a linear calibration curve for tested purine and pyrimidine derivatives within the range of 5 500 mumol/L (r > 0.996) The coefficients of variation for the within- and between-day imprecisions were less than 3.2 and 5.8%, respectively. Characteristic abnormalities were detected in the electropherograms of urine samples from patients with purine and pyrimidine enzyme deficiencies. We provide electrophoretic and spectral characteristics of i (en)
Title	Capillary electrophoresis for detection of inherited disorders of purine and pyrimidine metabolism: A selective approach Capillary electrophoresis for detection of inherited disorders of purine and pyrimidine metabolism: A selective approach (en)
skos:prefLabel	Capillary electrophoresis for detection of inherited disorders of purine and pyrimidine metabolism: A selective approach Capillary electrophoresis for detection of inherited disorders of purine and pyrimidine metabolism: A selective approach (en)
skos:notation	RIV/61989592:15310/02:00001497!RIV/2003/MSM/153103/N
http://linked.open.../vavai/riv/strany	565-571
http://linked.open...avai/riv/aktivita	Z
http://linked.open...avai/riv/aktivity	Z(MSM 153100013)
http://linked.open...iv/cisloPeriodika	4
http://linked.open...vai/riv/dodaniDat	2003
http://linked.open...aciTvurceVysledku	Barták, Petr Adam, Tomáš
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Palackého v Olomouci / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	640096
http://linked.open...ai/riv/idVysledku	RIV/61989592:15310/02:00001497
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	metabolism errors; purine; pyrimidine; screening; urine (en)
http://linked.open.../riv/klicoveSlovo	purine screening urine pyrimidine metabolism errors
http://linked.open...odStatuVydavatele	DE - Spolková republika Německo
http://linked.open...ontrolniKodProRIV	[534DCD513622]
http://linked.open...i/riv/nazevZdroje	Electrophoresis
http://linked.open...in/vavai/riv/obor	CB
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...ocetUcastnikuAkce	0 (xsd:int)
http://linked.open...nichUcastnikuAkce	0 (xsd:int)
http://linked.open...UplatneniVysledku	2002
http://linked.open...v/svazekPeriodika	23
http://linked.open...iv/tvurceVysledku	Barták, Petr Adam, Tomáš Friedecký, David
http://linked.open...n/vavai/riv/zamer	http://linked.opendata.cz/resource/domain/vavai/zamer/MSM%20153100013
issn	0173-0835
number of pages	7 (xsd:int)
http://localhost/t...ganizacniJednotka	15310

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