About: Effect of purine derivative myoseverin and of its analogues on cultured hybridoma cells

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About: Effect of purine derivative myoseverin and of its analogues on cultured hybridoma cells Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Two 2,6,9-trisubstituted purine derivatives, 9-isopropyl-2,6-bis[(4-methoxybenzyl) amino] 9H-purine (myoseverin, PMYO, 1) and 9-isopropyl-2,6-bis[(2-methoxybenzyl)amino]-9H-purine (OMYO, 2), and two 6,9-disubstituted derivatives, 9-isopropyl-6-[(4-methoxybenzyl)amino]-9H-purine (3) and 9-isopropyl-6-[(2-methoxybenzyl)amino]-9H-purine (4), were synthesized with the aim to examine their cell proliferation inhibiting activity, and possible additional effects in cultures of hybridoma cells producing monoclonal antibody. The substances were tested over a concentration range from 0.003 to 30 mumol l(-1). The most active compound 1 caused a total loss of cell viability at 1 mumol l(-1), while its isomer 2 showed the same effect at 10 mumol l(-1) concentration. In the presence of compound 1, but not of compound 2, the character of the cell cycle phases profile changed dramatically, most cells being arrested in the G(2)/M phase. At intermediate concentrations of compound 2 a substantially higher viable cell co Two 2,6,9-trisubstituted purine derivatives, 9-isopropyl-2,6-bis[(4-methoxybenzyl) amino] 9H-purine (myoseverin, PMYO, 1) and 9-isopropyl-2,6-bis[(2-methoxybenzyl)amino]-9H-purine (OMYO, 2), and two 6,9-disubstituted derivatives, 9-isopropyl-6-[(4-methoxybenzyl)amino]-9H-purine (3) and 9-isopropyl-6-[(2-methoxybenzyl)amino]-9H-purine (4), were synthesized with the aim to examine their cell proliferation inhibiting activity, and possible additional effects in cultures of hybridoma cells producing monoclonal antibody. The substances were tested over a concentration range from 0.003 to 30 mumol l(-1). The most active compound 1 caused a total loss of cell viability at 1 mumol l(-1), while its isomer 2 showed the same effect at 10 mumol l(-1) concentration. In the presence of compound 1, but not of compound 2, the character of the cell cycle phases profile changed dramatically, most cells being arrested in the G(2)/M phase. At intermediate concentrations of compound 2 a substantially higher viable cell co (en)
Title	Effect of purine derivative myoseverin and of its analogues on cultured hybridoma cells Effect of purine derivative myoseverin and of its analogues on cultured hybridoma cells (en)
skos:prefLabel	Effect of purine derivative myoseverin and of its analogues on cultured hybridoma cells Effect of purine derivative myoseverin and of its analogues on cultured hybridoma cells (en)
skos:notation	RIV/61989592:15310/02:00001491!RIV/2003/MSM/153103/N
http://linked.open.../vavai/riv/strany	257-266
http://linked.open...avai/riv/aktivita	Z
http://linked.open...avai/riv/aktivity	Z(MSM 153100008)
http://linked.open...iv/cisloPeriodika	2
http://linked.open...vai/riv/dodaniDat	2003
http://linked.open...aciTvurceVysledku	Strnad, Miroslav Weigl, Evžen
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Palackého v Olomouci / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	644293
http://linked.open...ai/riv/idVysledku	RIV/61989592:15310/02:00001491
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	purines; CDK inhibitors; cytokinins; cytostatics; hybridoma cells; monoclonal antibodies (en)
http://linked.open.../riv/klicoveSlovo	cytostatics purines hybridoma cells cytokinins monoclonal antibodies CDK inhibitors
http://linked.open...odStatuVydavatele	CZ - Česká republika
http://linked.open...ontrolniKodProRIV	[A0E89C593EB9]
http://linked.open...i/riv/nazevZdroje	Collection of Czechoslovak Chemical Communications
http://linked.open...in/vavai/riv/obor	ED
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...ocetUcastnikuAkce	0 (xsd:int)
http://linked.open...nichUcastnikuAkce	0 (xsd:int)
http://linked.open...UplatneniVysledku	2002
http://linked.open...v/svazekPeriodika	67
http://linked.open...iv/tvurceVysledku	Eckschlager, Tomáš Havlíček, Libor Strnad, Miroslav Weigl, Evžen Franěk, František Siglerová, Věra
http://linked.open...n/vavai/riv/zamer	http://linked.opendata.cz/resource/domain/vavai/zamer/MSM%20153100008
issn	0010-0765
number of pages	10 (xsd:int)
http://localhost/t...ganizacniJednotka	15310

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