About: Purification and characterization of amine oxidase and its endogenous inhibitor from Phaseolus vulgaris

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About: Purification and characterization of amine oxidase and its endogenous inhibitor from Phaseolus vulgaris Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Amine oxidase activity in the seedlings homogenates of common bean (Phaseolus vulgaris) was reported to be absent due to the presence of low-molecular endogenous inhibitor. We investigated the changes in amine oxidase activity in developing bean seedlings by the use of biosensor-immobilized tissue cuttings and by histochemical analysis. Amine oxidase was isolated and purified to apparent homogeneity from 5-days old etiolated seedlings. The isolation scheme included fractionation by ammonium sulphate, ion-exchange chromatography on DEAE-cellulose and hydrophobic chromatography on hydroxyapatite. Further purification was achieved through HPLC on ion-exchange and gel columns. Purified enzyme was characterised by native and SDS-electrophoresis. After native electrophoresis, the enzyme was transferred to cellulose acetate membranes by semi-dry blotting, but antibodies to amine oxidase from pea (Pisum sativum) did not show cross-reactivity with corresponding protein from Phaseolus. Spectroscopic properties Amine oxidase activity in the seedlings homogenates of common bean (Phaseolus vulgaris) was reported to be absent due to the presence of low-molecular endogenous inhibitor. We investigated the changes in amine oxidase activity in developing bean seedlings by the use of biosensor-immobilized tissue cuttings and by histochemical analysis. Amine oxidase was isolated and purified to apparent homogeneity from 5-days old etiolated seedlings. The isolation scheme included fractionation by ammonium sulphate, ion-exchange chromatography on DEAE-cellulose and hydrophobic chromatography on hydroxyapatite. Further purification was achieved through HPLC on ion-exchange and gel columns. Purified enzyme was characterised by native and SDS-electrophoresis. After native electrophoresis, the enzyme was transferred to cellulose acetate membranes by semi-dry blotting, but antibodies to amine oxidase from pea (Pisum sativum) did not show cross-reactivity with corresponding protein from Phaseolus. Spectroscopic properties (en) Byla provedena purifikace a charakterizace aminoxidasy a jejího endogenního inhibitoru z Phaseolus vulgaris. (cs)
Title	Purifikace a charakterizace aminoxidasy a jejího endogenního inhibitoru z Phaseolus vulgaris. (cs) Purification and characterization of amine oxidase and its endogenous inhibitor from Phaseolus vulgaris Purification and characterization of amine oxidase and its endogenous inhibitor from Phaseolus vulgaris (en)
skos:prefLabel	Purifikace a charakterizace aminoxidasy a jejího endogenního inhibitoru z Phaseolus vulgaris. (cs) Purification and characterization of amine oxidase and its endogenous inhibitor from Phaseolus vulgaris Purification and characterization of amine oxidase and its endogenous inhibitor from Phaseolus vulgaris (en)
skos:notation	RIV/61989592:15310/01:00002026!RIV/2005/GA0/153105/N
http://linked.open.../vavai/riv/strany	122
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(GA203/00/D119)
http://linked.open...vai/riv/dodaniDat	2005
http://linked.open...aciTvurceVysledku	Peč, Pavel Šebela, Marek Luhová, Lenka Lemr, Karel Petřivalský, Marek Zajoncová, Ludmila
http://linked.open.../riv/druhVysledku	D - Článek ve sborníku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Palackého v Olomouci / Přírodovědecká fakulta
http://linked.open...dnocenehoVysledku	693707
http://linked.open...ai/riv/idVysledku	RIV/61989592:15310/01:00002026
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	amine oxidase;enzyme;inhibitor;Phaseolus vulgaris (en)
http://linked.open.../riv/klicoveSlovo	Phaseolus vulgaris amine oxidase enzyme inhibitor
http://linked.open...ontrolniKodProRIV	[DC4556D7642F]
http://linked.open...i/riv/mistoVydani	Praha
http://linked.open...i/riv/nazevZdroje	Proceedings of 2nd International Symposium %22Separations in the BioSciences - SBS 2001%22
http://linked.open...in/vavai/riv/obor	CE
http://linked.open...ichTvurcuVysledku	6 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...vavai/riv/projekt	Structure and properties of endogenous amine oxidase inhibitor from bean seedlings and its analogues
http://linked.open...UplatneniVysledku	2001
http://linked.open...iv/tvurceVysledku	Lemr, Karel Petřivalský, Marek Šebela, Marek Luhová, Lenka Zajoncová, Ludmila Peč, Pavel
number of pages	159 (xsd:int)
http://purl.org/ne...btex#hasPublisher	Česká společnost chemická
https://schema.org/isbn	80-7080-437-8
http://localhost/t...ganizacniJednotka	15310

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