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  • Pythiosis is a rare infectious disease caused by Pythium insidiosum, which typically occurs in tropical and subtropical regions. The high mortality rate may be in consequence of the lack of diagnosis. The objective of this study was to evaluate reliability of a new single-tube nested PCR for detection of P. insidiosum DNA. A total of 78 clinical isolates of various fungi and bacteria, 106 clinical specimens and 80 simulated positive blood samples were tested. The developed primer pairs CPL6-CPR8 and YTL1-YTR1 are located on 18S subunit of the rRNA gene of P. insidiosum. The specificity, negative and positive predictive values were 100, 100 and 87.5 %, respectively, as compared with direct microscopy and cultivation. The detection limit of the single-tube nested PCR was 21 zoospores corresponding to 2.7 pg of the DNA. The results demonstrate that the new single-tube nested PCR offers a highly sensitive, specific and rapid genetic method for detecting P. insidiosum.
  • Pythiosis is a rare infectious disease caused by Pythium insidiosum, which typically occurs in tropical and subtropical regions. The high mortality rate may be in consequence of the lack of diagnosis. The objective of this study was to evaluate reliability of a new single-tube nested PCR for detection of P. insidiosum DNA. A total of 78 clinical isolates of various fungi and bacteria, 106 clinical specimens and 80 simulated positive blood samples were tested. The developed primer pairs CPL6-CPR8 and YTL1-YTR1 are located on 18S subunit of the rRNA gene of P. insidiosum. The specificity, negative and positive predictive values were 100, 100 and 87.5 %, respectively, as compared with direct microscopy and cultivation. The detection limit of the single-tube nested PCR was 21 zoospores corresponding to 2.7 pg of the DNA. The results demonstrate that the new single-tube nested PCR offers a highly sensitive, specific and rapid genetic method for detecting P. insidiosum. (en)
Title
  • Evalutation for the Clinical Diagnosis of Pythium insidiosum Using a Single-Tube Nested PCR
  • Evalutation for the Clinical Diagnosis of Pythium insidiosum Using a Single-Tube Nested PCR (en)
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  • Evalutation for the Clinical Diagnosis of Pythium insidiosum Using a Single-Tube Nested PCR
  • Evalutation for the Clinical Diagnosis of Pythium insidiosum Using a Single-Tube Nested PCR (en)
skos:notation
  • RIV/61989592:15110/13:33153310!RIV15-MSM-15110___
http://linked.open...avai/riv/aktivita
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  • RIV/61989592:15110/13:33153310
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  • Single-tube nested PCR.18S rRNA; Pythiosis; Pythium insidiosum (en)
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  • NL - Nizozemsko
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  • MYCOPATHOLOGIA
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  • 176
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  • Hamal, Petr
  • Svobodová, Lucie
  • Chaiprasert, Angkana
  • Pakarasang, Maitree
  • Prariyachatigul, Chularut
  • Thongsri, Yordhathai
  • Wonglakorn, Lumyai
http://linked.open...ain/vavai/riv/wos
  • 000328194100007
issn
  • 0301-486X
number of pages
http://bibframe.org/vocab/doi
  • 10.1007/s11046-013-9695-3
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  • 15110
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