About: Relationship between reactive oxygen species production in human semen and sperm DNA damage assessed by Sperm Chromatin Structure Assay

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About: Relationship between reactive oxygen species production in human semen and sperm DNA damage assessed by Sperm Chromatin Structure Assay Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

Attributes	Values
rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
rdfs:seeAlso	http://biomed.papers.upol.cz/pdfs/bio/2013/04/18.pdf
Description	The aim of this study was to find possible relationship between ROS production measured by chemiluminescence and flow cytometry in human semen and sperm DNA damage. Study included 39 men from infertile couples and 23 fertile volunteers who served as a control group. Aliquot of neat semen was used for ROS detection by chemiluminescence. Aliquot of sperm suspension in phosphate buffered saline was used for the detection of ROS by flow cytometry. Another aliquot of sperm suspension was used for SCSA to measure DNA fragmentation index and High DNA stainability. DNA fragmentation index correlated negatively with sperm morphology and motility. High DNA stainability correlated positively with ROS production and negatively with sperm morphology and concentration. Relationship between DNA fragmentation index and ROS production did not reach statistical significance. The aim of this study was to find possible relationship between ROS production measured by chemiluminescence and flow cytometry in human semen and sperm DNA damage. Study included 39 men from infertile couples and 23 fertile volunteers who served as a control group. Aliquot of neat semen was used for ROS detection by chemiluminescence. Aliquot of sperm suspension in phosphate buffered saline was used for the detection of ROS by flow cytometry. Another aliquot of sperm suspension was used for SCSA to measure DNA fragmentation index and High DNA stainability. DNA fragmentation index correlated negatively with sperm morphology and motility. High DNA stainability correlated positively with ROS production and negatively with sperm morphology and concentration. Relationship between DNA fragmentation index and ROS production did not reach statistical significance. (en)
Title	Relationship between reactive oxygen species production in human semen and sperm DNA damage assessed by Sperm Chromatin Structure Assay Relationship between reactive oxygen species production in human semen and sperm DNA damage assessed by Sperm Chromatin Structure Assay (en)
skos:prefLabel	Relationship between reactive oxygen species production in human semen and sperm DNA damage assessed by Sperm Chromatin Structure Assay Relationship between reactive oxygen species production in human semen and sperm DNA damage assessed by Sperm Chromatin Structure Assay (en)
skos:notation	RIV/61989592:15110/13:33146665!RIV14-MZ0-15110___
http://linked.open...avai/riv/aktivita	P S
http://linked.open...avai/riv/aktivity	P(NT11083), S
http://linked.open...iv/cisloPeriodika	4
http://linked.open...vai/riv/dodaniDat	2014
http://linked.open...aciTvurceVysledku	Řeřuchová, Marie Molíková, Radka Novotný, Jiří Březinová, Jana
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Palackého v Olomouci / Lékařská fakulta
http://linked.open...dnocenehoVysledku	102197
http://linked.open...ai/riv/idVysledku	RIV/61989592:15110/13:33146665
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	DNA damage; SCSA; ROS; human semen (en)
http://linked.open.../riv/klicoveSlovo	ROS DNA damage SCSA human semen
http://linked.open...odStatuVydavatele	CZ - Česká republika
http://linked.open...ontrolniKodProRIV	[840FF7D79D86]
http://linked.open...i/riv/nazevZdroje	Biomedical Papers-Olomouc
http://linked.open...in/vavai/riv/obor	FK
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	8 (xsd:int)
http://linked.open...vavai/riv/projekt	Selection of functional spermatozoa using chosen biomarkers in diagnosis of male infertility
http://linked.open...UplatneniVysledku	2013
http://linked.open...v/svazekPeriodika	157
http://linked.open...iv/tvurceVysledku	Březinová, Jana Novotný, Jiří Oborná, Ivana Rybář, Roman Kopecká, Věra Filipčíková, Radka Aziz, Nabil Řeřuchová, Marie
http://linked.open...ain/vavai/riv/wos	000329091500019
issn	1213-8118
number of pages	4 (xsd:int)
http://bibframe.org/vocab/doi	10.5507/bp.2013.065
http://localhost/t...ganizacniJednotka	15110

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