About: A New Method of the Visualization of the Double-Stranded Mitochondrial and Nuclear DNA

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About: A New Method of the Visualization of the Double-Stranded Mitochondrial and Nuclear DNA Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	The study describes the method of a sensitive detection of double-stranded DNA molecules in situ. It is based on the oxidative attack on the deoxyribose moiety by copper(I) in the presence of oxygen. We have shown previously that the oxidative attack leads to the formation of frequent gaps in DNA. Here we have demonstrated that the gaps can be utilized as the origins for an efficient synthesis of complementary labeled strands by DNA polymerase I and that such enzymatic detection of the double-stranded DNA is a sensitive approach enabling in-situ detection of both the nuclear and mitochondrial genomes in formaldehyde-fixed human cells. The study describes the method of a sensitive detection of double-stranded DNA molecules in situ. It is based on the oxidative attack on the deoxyribose moiety by copper(I) in the presence of oxygen. We have shown previously that the oxidative attack leads to the formation of frequent gaps in DNA. Here we have demonstrated that the gaps can be utilized as the origins for an efficient synthesis of complementary labeled strands by DNA polymerase I and that such enzymatic detection of the double-stranded DNA is a sensitive approach enabling in-situ detection of both the nuclear and mitochondrial genomes in formaldehyde-fixed human cells. (en)
Title	A New Method of the Visualization of the Double-Stranded Mitochondrial and Nuclear DNA A New Method of the Visualization of the Double-Stranded Mitochondrial and Nuclear DNA (en)
skos:prefLabel	A New Method of the Visualization of the Double-Stranded Mitochondrial and Nuclear DNA A New Method of the Visualization of the Double-Stranded Mitochondrial and Nuclear DNA (en)
skos:notation	RIV/61989592:15110/13:33143922!RIV15-TA0-15110___
http://linked.open...avai/riv/aktivita	P
http://linked.open...avai/riv/aktivity	P(ED0030/01/01), P(TA03010598), P(TA03010719)
http://linked.open...iv/cisloPeriodika	6
http://linked.open...vai/riv/dodaniDat	2015
http://linked.open...aciTvurceVysledku	Koberna, Karel Ligasová, Anna
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Palackého v Olomouci / Lékařská fakulta
http://linked.open...dnocenehoVysledku	58826
http://linked.open...ai/riv/idVysledku	RIV/61989592:15110/13:33143922
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	copper(I) ions; light microscopy; mitochondrial DNA; nuclear DNA (en)
http://linked.open.../riv/klicoveSlovo	mitochondrial DNA light microscopy copper(I) ions nuclear DNA
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[2D524CFE2273]
http://linked.open...i/riv/nazevZdroje	PLoS One
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	3 (xsd:int)
http://linked.open...vavai/riv/projekt	Biomedicine for regional development and human resources Modified nucleosides, nucleotides and oligonucleotides and their application in biomedicine Novel Tools for Cell Cycle Analysis
http://linked.open...UplatneniVysledku	2013
http://linked.open...v/svazekPeriodika	8
http://linked.open...iv/tvurceVysledku	Koberna, Karel Ligasová, Anna Strunin, Dmytro
http://linked.open...ain/vavai/riv/wos	000320576400163
issn	1932-6203
number of pages	6 (xsd:int)
http://bibframe.org/vocab/doi	10.1371/journal.pone.0066864
http://localhost/t...ganizacniJednotka	15110

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