About: Assay for determination of daunorubicin in cancer cells with multidrug resistance phenotype

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	A sensitive assay for direct determination of intracellular level of daunorubicin (DRN) in resistant leukemia cells with overexpressed P-glycoprotein has been developed. This assay is based on a rapid separation of cells from media and fast cut-off of DRN transportation by centrifugation of cells through a layer of silicone oil. Cell pellets were extracted using 1% (v/v) formic acid in 50% (v/v) ethanol in water. The cell extracts were subsequently analysed by liquid chromatography (HPLC) coupled a low-energy collision tandem mass spectrometer equipped with an electrospray ionization source (ESI-CID-MS/MS) operated in the multiple-reaction monitoring (MRM) mode. Calibration curve was linear from 0.4 to 250nM with correlation coefficient (r2) better than 0.998. The limit of quantitation (LOQ) was 0.4 nM. The assay has been successfully applied to a determination of intracellular content of daunorubicin in sensitive K562 and resistant K562/Dox and K562/HHT300 cells. A sensitive assay for direct determination of intracellular level of daunorubicin (DRN) in resistant leukemia cells with overexpressed P-glycoprotein has been developed. This assay is based on a rapid separation of cells from media and fast cut-off of DRN transportation by centrifugation of cells through a layer of silicone oil. Cell pellets were extracted using 1% (v/v) formic acid in 50% (v/v) ethanol in water. The cell extracts were subsequently analysed by liquid chromatography (HPLC) coupled a low-energy collision tandem mass spectrometer equipped with an electrospray ionization source (ESI-CID-MS/MS) operated in the multiple-reaction monitoring (MRM) mode. Calibration curve was linear from 0.4 to 250nM with correlation coefficient (r2) better than 0.998. The limit of quantitation (LOQ) was 0.4 nM. The assay has been successfully applied to a determination of intracellular content of daunorubicin in sensitive K562 and resistant K562/Dox and K562/HHT300 cells. (en)
Title	Assay for determination of daunorubicin in cancer cells with multidrug resistance phenotype Assay for determination of daunorubicin in cancer cells with multidrug resistance phenotype (en)
skos:prefLabel	Assay for determination of daunorubicin in cancer cells with multidrug resistance phenotype Assay for determination of daunorubicin in cancer cells with multidrug resistance phenotype (en)
skos:notation	RIV/61989592:15110/11:33118991!RIV12-MZ0-15110___
http://linked.open...avai/riv/aktivita	P S Z
http://linked.open...avai/riv/aktivity	P(NR9482), S, Z(MSM6198959216)
http://linked.open...iv/cisloPeriodika	21
http://linked.open...vai/riv/dodaniDat	2012
http://linked.open...aciTvurceVysledku	KRUMPOCHOVÁ, Petra MLEJNEK, Petr DOLEŽEL, Petr
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Palackého v Olomouci / Lékařská fakulta
http://linked.open...dnocenehoVysledku	187212
http://linked.open...ai/riv/idVysledku	RIV/61989592:15110/11:33118991
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	silicone oil; ABCB1; P-glycoprotein; daunomycin; K562 cells; Intracellular drug level (en)
http://linked.open.../riv/klicoveSlovo	ABCB1 Intracellular drug level daunomycin K562 cells P-glycoprotein silicone oil
http://linked.open...odStatuVydavatele	NL - Nizozemsko
http://linked.open...ontrolniKodProRIV	[9E2034C62D12]
http://linked.open...i/riv/nazevZdroje	JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
http://linked.open...in/vavai/riv/obor	CB
http://linked.open...ichTvurcuVysledku	3 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...vavai/riv/projekt	P-glycoprotein and its possible contribution to development of resistance to Imatinib in Bcr-Abl expressing cells
http://linked.open...UplatneniVysledku	2011
http://linked.open...v/svazekPeriodika	879
http://linked.open...iv/tvurceVysledku	DOLEŽEL, Petr KRUMPOCHOVÁ, Petra Kocúrová, Andrea MLEJNEK, Petr
http://linked.open...n/vavai/riv/zamer	Modulation of signalling and regulatory pathways in normal and cancer cells
issn	1570-0232
number of pages	6 (xsd:int)
http://bibframe.org/vocab/doi	10.1016/j.jchromb.2011.05.008
http://localhost/t...ganizacniJednotka	15110

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