About: Comparison of chemiluminescence and flow cytometry in the estimation of reactive oxygen and nitrogen species in human semen

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About: Comparison of chemiluminescence and flow cytometry in the estimation of reactive oxygen and nitrogen species in human semen Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	OBJECTIVE: To determine the relative contribution of different cell types in washed sperm to the overall intracellular production of H(2)O(2) and peroxynitrite. SETTING: University hospital. MAIN OUTCOME MEASURE(S): Simultaneous estimates of global ROS production assessed by chemiluminescence assay compared with flow cytometric measurements. RESULT(S): The estimates of ROS with use of chemiluminescence positively correlated with the estimates of H(2)O(2) (r = 0.53) and peroxynitrite (r = 0.62) as assessed with flow cytometry. H(2)O(2) and peroxynitrite were measurable also in samples in which chemiluminescence did not detect measurable values. Increased production of H(2)O(2) by one cell type was associated with a relative increase in its peroxynitrite production. OBJECTIVE: To determine the relative contribution of different cell types in washed sperm to the overall intracellular production of H(2)O(2) and peroxynitrite. SETTING: University hospital. MAIN OUTCOME MEASURE(S): Simultaneous estimates of global ROS production assessed by chemiluminescence assay compared with flow cytometric measurements. RESULT(S): The estimates of ROS with use of chemiluminescence positively correlated with the estimates of H(2)O(2) (r = 0.53) and peroxynitrite (r = 0.62) as assessed with flow cytometry. H(2)O(2) and peroxynitrite were measurable also in samples in which chemiluminescence did not detect measurable values. Increased production of H(2)O(2) by one cell type was associated with a relative increase in its peroxynitrite production. (en)
Title	Comparison of chemiluminescence and flow cytometry in the estimation of reactive oxygen and nitrogen species in human semen Comparison of chemiluminescence and flow cytometry in the estimation of reactive oxygen and nitrogen species in human semen (en)
skos:prefLabel	Comparison of chemiluminescence and flow cytometry in the estimation of reactive oxygen and nitrogen species in human semen Comparison of chemiluminescence and flow cytometry in the estimation of reactive oxygen and nitrogen species in human semen (en)
skos:notation	RIV/61989592:15110/10:10212108!RIV11-MZ0-15110___
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(1A8621), Z(MSM6198959223)
http://linked.open...iv/cisloPeriodika	7
http://linked.open...vai/riv/dodaniDat	2011
http://linked.open...aciTvurceVysledku	Novotný, Jiří Oborná, Ivana Fingerová, Helena Březinová, Jana
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Palackého v Olomouci / Lékařská fakulta
http://linked.open...dnocenehoVysledku	251394
http://linked.open...ai/riv/idVysledku	RIV/61989592:15110/10:10212108
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	leukocytes; round cells; flow cytometry; chemiluminescence; ROS; human semen; Male infertility (en)
http://linked.open.../riv/klicoveSlovo	ROS leukocytes chemiluminescence flow cytometry Male infertility human semen round cells
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[FEC8CAE47827]
http://linked.open...i/riv/nazevZdroje	Fertility and Sterility
http://linked.open...in/vavai/riv/obor	FK
http://linked.open...ichTvurcuVysledku	4 (xsd:int)
http://linked.open...cetTvurcuVysledku	6 (xsd:int)
http://linked.open...vavai/riv/projekt	Oxidative stress in pathophysiology of male infertility.
http://linked.open...UplatneniVysledku	2010
http://linked.open...v/svazekPeriodika	94
http://linked.open...iv/tvurceVysledku	Březinová, Jana Fingerová, Helena Novotný, Jiří Oborná, Ivana Svobodová, Magda Aziz, Nabil
http://linked.open...n/vavai/riv/zamer	Nové možnosti diagnostiky a imunomodulace u infekčních onemocnění a imunopatologických stavů
issn	0015-0282
number of pages	5 (xsd:int)
http://localhost/t...ganizacniJednotka	15110

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