About: Low-dose DNA damage and replication stress responses quantified by optimized automated single-cell image analysis

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About: Low-dose DNA damage and replication stress responses quantified by optimized automated single-cell image analysis Goto Sponge NotDistinct Permalink

An Entity of Type : http://linked.opendata.cz/ontology/domain/vavai/Vysledek, within Data Space : linked.opendata.cz associated with source document(s)

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Maintenance of genome integrity is essential for homeostasis and survival as impaired DNA damage response (DDR) may predispose to grave pathologies such as neurodegenerative and immunodeficiency syndromes, cancer and premature aging. Therefore, accurate assessment of DNA damage caused by environmental or metabolic genotoxic insults is critical for contemporary biomedicine. The available physical, flow cytometry and sophisticated scanning approaches to DNA damage estimation each have some drawbacks such as insufficient sensitivity, limitation to analysis of cells in suspension, or high costs and demand for trained personnel. Here we present an option how to transform a regular fluorescence microscope and personal computer with common software into a functional alternative to high-throughput screening devices. In two detailed protocols we introduce a new semi-automatic procedure allowing for very sensitive, quantitative, rapid and simple fluorescence image analysis in thousands of adherent cells per day Maintenance of genome integrity is essential for homeostasis and survival as impaired DNA damage response (DDR) may predispose to grave pathologies such as neurodegenerative and immunodeficiency syndromes, cancer and premature aging. Therefore, accurate assessment of DNA damage caused by environmental or metabolic genotoxic insults is critical for contemporary biomedicine. The available physical, flow cytometry and sophisticated scanning approaches to DNA damage estimation each have some drawbacks such as insufficient sensitivity, limitation to analysis of cells in suspension, or high costs and demand for trained personnel. Here we present an option how to transform a regular fluorescence microscope and personal computer with common software into a functional alternative to high-throughput screening devices. In two detailed protocols we introduce a new semi-automatic procedure allowing for very sensitive, quantitative, rapid and simple fluorescence image analysis in thousands of adherent cells per day (en)
Title	Low-dose DNA damage and replication stress responses quantified by optimized automated single-cell image analysis Low-dose DNA damage and replication stress responses quantified by optimized automated single-cell image analysis (en)
skos:prefLabel	Low-dose DNA damage and replication stress responses quantified by optimized automated single-cell image analysis Low-dose DNA damage and replication stress responses quantified by optimized automated single-cell image analysis (en)
skos:notation	RIV/61989592:15110/09:00009749!RIV10-MSM-15110___
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(IAA501370902), Z(MSM6198959216)
http://linked.open...iv/cisloPeriodika	16
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Bártek, Jiří Mistrík, Martin
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Palackého v Olomouci / Lékařská fakulta
http://linked.open...dnocenehoVysledku	324188
http://linked.open...ai/riv/idVysledku	RIV/61989592:15110/09:00009749
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	DNA damage; replication stress; γ-H2AX; Rad51 foci; cancer cells; image analysis; homologous recombination; DNA breakage repair kinetics; cell cycle (en)
http://linked.open.../riv/klicoveSlovo	γ-H2AX homologous recombination DNA damage image analysis cancer cells cell cycle DNA breakage repair kinetics Rad51 foci replication stress
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[C19F5176C8CF]
http://linked.open...i/riv/nazevZdroje	Cell cycle
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	4 (xsd:int)
http://linked.open...vavai/riv/projekt	Cellular responses to replication stress: Mechanisms and the role of fragile sites in genomic instability
http://linked.open...UplatneniVysledku	2009
http://linked.open...v/svazekPeriodika	8
http://linked.open...iv/tvurceVysledku	Bártek, Jiří Mistrík, Martin Lukáš, Jiří Opluštilová, Lenka
http://linked.open...n/vavai/riv/zamer	Modulation of signalling and regulatory pathways in normal and cancer cells
issn	1538-4101
number of pages	8 (xsd:int)
http://localhost/t...ganizacniJednotka	15110

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