About: Human Fbh1 helicase contributes to genome maintenance via pro- and anti-recombinase activities

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About: Human Fbh1 helicase contributes to genome maintenance via pro- and anti-recombinase activities Goto Sponge NotDistinct Permalink

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rdf:type	skos:Concept http://linked.opendata.cz/ontology/domain/vavai/Vysledek
Description	Homologous recombination (HR) is essential for faithful repair of DNA lesions yet must be kept in check, as unrestrained HR may compromise genome integrity and lead to premature aging or cancer. To limit unscheduled HR, cells possess DNA helicases capable of preventing excessive recombination. In this study, we show that the human Fbh1 (hFbh1) helicase accumulates at sites of DNA damage or replication stress in a manner dependent fully on its helicase activity and partially on its conserved F box. hFbh1 interacted with singlestranded DNA (ssDNA), the formation of which was required for hFbh1 recruitment to DNA lesions. Conversely, depletion of endogenous Fbh1 or ectopic expression of helicase-deficient hFbh1 attenuated ssDNA production after replication block. Although elevated levels of hFbh1 impaired Rad51 recruitment to ssDNA and suppressed HR, its small interfering RNA?mediated depletion increased the levels of chromatin associated Rad51 and caused unscheduled sister chromatid exchange. Thus, by Homologous recombination (HR) is essential for faithful repair of DNA lesions yet must be kept in check, as unrestrained HR may compromise genome integrity and lead to premature aging or cancer. To limit unscheduled HR, cells possess DNA helicases capable of preventing excessive recombination. In this study, we show that the human Fbh1 (hFbh1) helicase accumulates at sites of DNA damage or replication stress in a manner dependent fully on its helicase activity and partially on its conserved F box. hFbh1 interacted with singlestranded DNA (ssDNA), the formation of which was required for hFbh1 recruitment to DNA lesions. Conversely, depletion of endogenous Fbh1 or ectopic expression of helicase-deficient hFbh1 attenuated ssDNA production after replication block. Although elevated levels of hFbh1 impaired Rad51 recruitment to ssDNA and suppressed HR, its small interfering RNA?mediated depletion increased the levels of chromatin associated Rad51 and caused unscheduled sister chromatid exchange. Thus, by (en)
Title	Human Fbh1 helicase contributes to genome maintenance via pro- and anti-recombinase activities Human Fbh1 helicase contributes to genome maintenance via pro- and anti-recombinase activities (en)
skos:prefLabel	Human Fbh1 helicase contributes to genome maintenance via pro- and anti-recombinase activities Human Fbh1 helicase contributes to genome maintenance via pro- and anti-recombinase activities (en)
skos:notation	RIV/61989592:15110/09:00009747!RIV10-MSM-15110___
http://linked.open...avai/riv/aktivita	P Z
http://linked.open...avai/riv/aktivity	P(IAA501370902), Z(MSM6198959216)
http://linked.open...iv/cisloPeriodika	5
http://linked.open...vai/riv/dodaniDat	2010
http://linked.open...aciTvurceVysledku	Bártek, Jiří Mistrík, Martin
http://linked.open.../riv/druhVysledku	J - Článek v odborném periodiku
http://linked.open...iv/duvernostUdaju	S - Úplné a pravdivé údaje nepodléhající ochraně podle zvláštních právních předpisů
http://linked.open...titaPredkladatele	Univerzita Palackého v Olomouci / Lékařská fakulta
http://linked.open...dnocenehoVysledku	318024
http://linked.open...ai/riv/idVysledku	RIV/61989592:15110/09:00009747
http://linked.open...riv/jazykVysledku	eng - angličtina
http://linked.open.../riv/klicovaSlova	Homologous recombination; DNA repair; genome integrity; cancer; DNA helicases; replication stress; single-stranded DNA (en)
http://linked.open.../riv/klicoveSlovo	cancer Homologous recombination DNA repair genome integrity DNA helicases single-stranded DNA replication stress
http://linked.open...odStatuVydavatele	US - Spojené státy americké
http://linked.open...ontrolniKodProRIV	[13D0FD9EDF38]
http://linked.open...i/riv/nazevZdroje	Journal of Cell Biology
http://linked.open...in/vavai/riv/obor	EB
http://linked.open...ichTvurcuVysledku	2 (xsd:int)
http://linked.open...cetTvurcuVysledku	8 (xsd:int)
http://linked.open...vavai/riv/projekt	Cellular responses to replication stress: Mechanisms and the role of fragile sites in genomic instability
http://linked.open...UplatneniVysledku	2009
http://linked.open...v/svazekPeriodika	186
http://linked.open...iv/tvurceVysledku	Bártek, Jiří Mistrík, Martin Lukáš, Jiří Fugger, Kasper Mailand, Niels Dinant, Christoffel Falck, Jacob Rendtlew Danielsen, Jannie
http://linked.open...n/vavai/riv/zamer	Modulation of signalling and regulatory pathways in normal and cancer cells
issn	0021-9525
number of pages	9 (xsd:int)
http://localhost/t...ganizacniJednotka	15110

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