| Attributes | Values |
|---|
| rdf:type
| |
| Description
| - Abstrakt. DFFa podobný faktor indukující buněčnou smrt, CIDEa, může iniciovat apoptózu rozložením komplexu sestávajícího z nukleázy aktivující kaspázu-3 o velikosti 40-kDa (DFF40/CAD) a jeho inhibitoru o velikosti 45-kDa (DFF45/ICAD). Nicméně bylo zjištěno, že se CIDEa nachází v mitochodnriích. Provedli jsme imunodetekci CIDEa v celých buňkách a subcelulárních frakcích HeLa buněk adaptovaných na tetracyklinem indukovanou expresi CIDEa. S využitím imunocytochemie jsem pozorovali redistribuci CIDEa do jádra, zvýšenou působením camptothecinu nebo valinomycinu. Podobně byl obsah CIDEa zvýšený v nukleární frakci a naopak snížený v cytosolické frakci v buňkách vedoucí k iniciaci apoptózy. Hypotéza zní, že CIDEa je osamocená v mitochondriích, přičemž přenos tohoto potenciálně nebezpečného proteinu z mitochondrií do jádra zvyšuje nebo dokonce iniciuje apoptózu. (cs)
- Cell death-inducing DFF[DNA fragmentation factor]-like effector-a (CIDEa), may initiate apoptosis by disrupting a complex consisting of 40-kDa caspase-3-activated nuclease (DFF40/CAD) and its 45-kDa inhibitor (DFF45/ICAD). CIDEa, however, was found to be localized in mitochondria. We have performed immunodetection of CIDEa in whole cells and subcellular fractions of HeLa cells adapted for a tetracycline-inducible CIDEa expression. Using immunocytochemistry we observed redistribution, enhanced upon treatment with camptothecin or valinomycin, of CIDEa to nucleus. Similarly, CIDEa content increased in the nuclear fraction but decreased in cytosolic fraction in cells treated to initiate apoptosis. We hypothesize that CIDEa is sequestered in mitochondria while transfer of this potentially dangerous protein from mitochondria into nucleus intensifies or even initiates apoptosis.
- Cell death-inducing DFF[DNA fragmentation factor]-like effector-a (CIDEa), may initiate apoptosis by disrupting a complex consisting of 40-kDa caspase-3-activated nuclease (DFF40/CAD) and its 45-kDa inhibitor (DFF45/ICAD). CIDEa, however, was found to be localized in mitochondria. We have performed immunodetection of CIDEa in whole cells and subcellular fractions of HeLa cells adapted for a tetracycline-inducible CIDEa expression. Using immunocytochemistry we observed redistribution, enhanced upon treatment with camptothecin or valinomycin, of CIDEa to nucleus. Similarly, CIDEa content increased in the nuclear fraction but decreased in cytosolic fraction in cells treated to initiate apoptosis. We hypothesize that CIDEa is sequestered in mitochondria while transfer of this potentially dangerous protein from mitochondria into nucleus intensifies or even initiates apoptosis. (en)
|
| Title
| - Redistribution of cell death-inducing DNA fragmentation factor-like effector-a (CIDEa) from mitochondria to nucleus is associated with apoptosis in HeLa cells
- Redistribution of cell death-inducing DNA fragmentation factor-like effector-a (CIDEa) from mitochondria to nucleus is associated with apoptosis in HeLa cells (en)
- Redistribuce CIDEa (DFFa podobný faktor indukující buněčnou smrt) z mitochondrií do jádra v HeLa buňkách (cs)
|
| skos:prefLabel
| - Redistribution of cell death-inducing DNA fragmentation factor-like effector-a (CIDEa) from mitochondria to nucleus is associated with apoptosis in HeLa cells
- Redistribution of cell death-inducing DNA fragmentation factor-like effector-a (CIDEa) from mitochondria to nucleus is associated with apoptosis in HeLa cells (en)
- Redistribuce CIDEa (DFFa podobný faktor indukující buněčnou smrt) z mitochondrií do jádra v HeLa buňkách (cs)
|
| skos:notation
| - RIV/61989592:15110/08:00007375!RIV09-MSM-15110___
|
| http://linked.open...avai/riv/aktivita
| |
| http://linked.open...avai/riv/aktivity
| |
| http://linked.open...iv/cisloPeriodika
| |
| http://linked.open...vai/riv/dodaniDat
| |
| http://linked.open...aciTvurceVysledku
| |
| http://linked.open.../riv/druhVysledku
| |
| http://linked.open...iv/duvernostUdaju
| |
| http://linked.open...titaPredkladatele
| |
| http://linked.open...dnocenehoVysledku
| |
| http://linked.open...ai/riv/idVysledku
| - RIV/61989592:15110/08:00007375
|
| http://linked.open...riv/jazykVysledku
| |
| http://linked.open.../riv/klicovaSlova
| - CIDEa; Mitochondria; Apoptosis; Migration to nucleus; HeLa cells (en)
|
| http://linked.open.../riv/klicoveSlovo
| |
| http://linked.open...odStatuVydavatele
| |
| http://linked.open...ontrolniKodProRIV
| |
| http://linked.open...i/riv/nazevZdroje
| - General Physiology and Biophysics
|
| http://linked.open...in/vavai/riv/obor
| |
| http://linked.open...ichTvurcuVysledku
| |
| http://linked.open...cetTvurcuVysledku
| |
| http://linked.open...UplatneniVysledku
| |
| http://linked.open...v/svazekPeriodika
| |
| http://linked.open...iv/tvurceVysledku
| - Ježek, Petr
- Modrianský, Martin
- Šantorová, Jitka
- Valoušková, Eva
- Smolková, Katarina
|
| http://linked.open...n/vavai/riv/zamer
| |
| issn
| |
| number of pages
| |
| http://localhost/t...ganizacniJednotka
| |
![[RDF Data]](/fct/images/sw-rdf-blue.png)
![[cxml]](/fct/images/cxml_doc.png)
![[csv]](/fct/images/csv_doc.png)
![[text]](/fct/images/ntriples_doc.png)
![[turtle]](/fct/images/n3turtle_doc.png)
![[ld+json]](/fct/images/jsonld_doc.png)
![[rdf+json]](/fct/images/json_doc.png)
![[rdf+xml]](/fct/images/xml_doc.png)
![[atom+xml]](/fct/images/atom_doc.png)
![[html]](/fct/images/html_doc.png)