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| Description
| - A quaternary benzo[c]phenanthridine alkaloid chelerythrine displays a wide range of biological activities including cytotoxicity to normal and cancer cells. In contrast, less is known about the biological activity of dihydrochelerythrine, a product of chelerythrine reduction. We examined the cytotoxicity of chelerythrine and dihydrochelerythrine in human promyelocytic leukemia HL-60 cells. After 4h of treatment, chelerythrine induced a dose-dependent decrease in the cell viability with IC50 of 2.6 microM as shown by MTT reduction assay. Dihydrochelerythrine appeared to be less cytotoxic since the viability of cells exposed to 20 microM dihydrochelerythrine for 24h was reduced only to 53%. Decrease in the viability induced by both alkaloids was accompanied by apoptotic events including the dissipation of mitochondrial membrane potential, activation of caspase-9 and -3, and appearance of cells with sub-G1 DNA. Moreover, chelerythrine, but not dihydrochelerythrine, elevated the activity of caspase-8. A d
- A quaternary benzo[c]phenanthridine alkaloid chelerythrine displays a wide range of biological activities including cytotoxicity to normal and cancer cells. In contrast, less is known about the biological activity of dihydrochelerythrine, a product of chelerythrine reduction. We examined the cytotoxicity of chelerythrine and dihydrochelerythrine in human promyelocytic leukemia HL-60 cells. After 4h of treatment, chelerythrine induced a dose-dependent decrease in the cell viability with IC50 of 2.6 microM as shown by MTT reduction assay. Dihydrochelerythrine appeared to be less cytotoxic since the viability of cells exposed to 20 microM dihydrochelerythrine for 24h was reduced only to 53%. Decrease in the viability induced by both alkaloids was accompanied by apoptotic events including the dissipation of mitochondrial membrane potential, activation of caspase-9 and -3, and appearance of cells with sub-G1 DNA. Moreover, chelerythrine, but not dihydrochelerythrine, elevated the activity of caspase-8. A d (en)
- Kvarterní benzo[c]fenanthridinový alkaloid chelerythrin vykazuje řadu biologických aktivit včetně cytotoxicity vůči normálním a nádorovým buňkám. Méně je však známo o biologické aktivitě dihydrochelerythrinu, produktu redukce chelerythrinu. My jsme se zabývali srovnáním cytotoxicity chelerythrinu a dihydrochelerythrinu v leukemických buňkách HL-60. Při inkubaci po dobu 4 h chelerythrin snižoval životnost buněk, přičemž hodnota IC50 získaná MTT metodou byla 2,6 ?mol/l. Dihydrochelerythrin byl méně cytotoxický, když při koncentraci 20 ?mol/l snížil po 24 h životnost pouze na 53%. Pokles životnosti vlivem obou alkaloidů byl doprovázen apoptotickými změnami zahrnujícími ztrátu mitochondriálního membránového poteciálu, aktivaci kaspasy-9 a -3 a degradaci DNA. Pouze chelerythrin navíc zvýšil také aktivitu kaspasy-8. Indukce apoptosy a nekrosy vlivem chelerythrinu a dihydrochelerythrinu byla potvrzena průtokovou cytometrií využívající značení buněk annexinem V a propidium jodidem. Oba alkaloidy také způsobov (cs)
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| Title
| - Chelerythrine and dihydrochelerythrine induce G1 phase arrest and bimodal cell death in human leukemia HL-60 cells
- Chelerythrin a dihydrochelerythrin způsobují v buňkách HL-60 zastavení buněčného cyklu v G1 fázi a bimodální buněčnou smrt (cs)
- Chelerythrine and dihydrochelerythrine induce G1 phase arrest and bimodal cell death in human leukemia HL-60 cells (en)
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| skos:prefLabel
| - Chelerythrine and dihydrochelerythrine induce G1 phase arrest and bimodal cell death in human leukemia HL-60 cells
- Chelerythrin a dihydrochelerythrin způsobují v buňkách HL-60 zastavení buněčného cyklu v G1 fázi a bimodální buněčnou smrt (cs)
- Chelerythrine and dihydrochelerythrine induce G1 phase arrest and bimodal cell death in human leukemia HL-60 cells (en)
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| skos:notation
| - RIV/61989592:15110/08:00006999!RIV09-MSM-15110___
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
| - P(GP303/06/P193), Z(MSM6198959216)
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/61989592:15110/08:00006999
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - Apoptosis; Cell cycle; Chelerythrine; Cytotoxicity; Dihydrochelerythrine; HL-60 cells; Mitochondrial membrane potential (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...odStatuVydavatele
| - GB - Spojené království Velké Británie a Severního Irska
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
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| http://linked.open...in/vavai/riv/obor
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| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...vavai/riv/projekt
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| http://linked.open...UplatneniVysledku
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| http://linked.open...v/svazekPeriodika
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| http://linked.open...iv/tvurceVysledku
| - Doležel, Petr
- Ulrichová, Jitka
- Vrba, Jiří
- Modrianský, Martin
- Vičar, Jaroslav
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| http://linked.open...n/vavai/riv/zamer
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| issn
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| number of pages
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| http://localhost/t...ganizacniJednotka
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