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| Description
| - Velmi malé změny mRNA stability mohou ovlivnit rozhodující body buněčných energetických drah. Cílem této studie bylo prozkoumat vliv energeticky bohatých substrátů na stálé hladiny mRNA receptorů aktivovaných proliferátory peroxisomů (PPARs) a PPAR-gamma koktivátorů (PGCs). Byla provedena kvantitativní RT-PCR studie pro vyhodnocení vlivu nulové nebo normální (5 mmol/l) glukózy a kyseliny olejové (0,3 mmol/l) na hladiny mRNA (PPARs) (PGCs) v hepatomových HepG2 buňkách. Hladiny mRNA. PGC-1alfa byly signifikantně zvýšené v buňkách ovlivněných nedostatkem glukózy (123% kontrolní hodnoty; p < 0,05), zatímco hladiny mRNA PGC 1beta byly signifikantně zvýšené v buňkách ovlivněných kyselinou olejovou (134% a 160% kontrolní hodnoty v buňkách ovlivněných nulovou glukózou s kyselinou olejovou a ovlivněných normální glukózou s kyselinou olejovou; p < 0,05) během 0,5 h inkubace. Po 4 h inkubaci byly signifikantně zvýšené hladiny mRNA PPAR-gamma a PGC-1alfa v buňkách s nulovou glukózou (142% a 163% kontrolní (cs)
- Very modest changes in mRNA stability can aff ect critical points in cellular energy pathways. The aim of this study was to investigate the impact of energy abundant substrates on peroxisome proliferator-activated receptors (PPARs) and PPAR-. coactivators (PGCs) mRNA?s steady-state levels. Quantitative RT-PCR study was performed to assess the effect of zero or normal (5 mmol/l) glucose and/or oleic acid (0.3 mmol/l) on mRNA levels of (PPARs) (PGCs) in HepG2 cells. PGC-1alfa mRNA was significantly upregulated in glucose deprived cells (123 % of the control level; p < 0.05), while PGC-1ß mRNA was significantly enhanced in oleate-fed cells (134 % and 160 % of control levels for zero glucose plus oleate and normal glucose plus oleate, respectively; p < 0.05) during the 0.5 h incubation. Upon the 4 h incubation, PPAR-gamma1 and PGC-1alfa mRNAs were significantly elevated in cells lacking glucose (142 % and 163 % of control levels, respectively; p < 0.05). Oleate significantly suppressed PPAR-al
- Very modest changes in mRNA stability can aff ect critical points in cellular energy pathways. The aim of this study was to investigate the impact of energy abundant substrates on peroxisome proliferator-activated receptors (PPARs) and PPAR-. coactivators (PGCs) mRNA?s steady-state levels. Quantitative RT-PCR study was performed to assess the effect of zero or normal (5 mmol/l) glucose and/or oleic acid (0.3 mmol/l) on mRNA levels of (PPARs) (PGCs) in HepG2 cells. PGC-1alfa mRNA was significantly upregulated in glucose deprived cells (123 % of the control level; p < 0.05), while PGC-1ß mRNA was significantly enhanced in oleate-fed cells (134 % and 160 % of control levels for zero glucose plus oleate and normal glucose plus oleate, respectively; p < 0.05) during the 0.5 h incubation. Upon the 4 h incubation, PPAR-gamma1 and PGC-1alfa mRNAs were significantly elevated in cells lacking glucose (142 % and 163 % of control levels, respectively; p < 0.05). Oleate significantly suppressed PPAR-al (en)
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| Title
| - mRNA levels of peroxisome proliferator-activated receptors and their coactivators are affected by glucose deprivation and oleate in heuman hepatoma HepG2 cells
- mRNA levels of peroxisome proliferator-activated receptors and their coactivators are affected by glucose deprivation and oleate in heuman hepatoma HepG2 cells (en)
- Hladiny mRNA receptorů aktivovaných proliferátory peroxisomů a jejich koaktivátorů jsou ovlivněny nedostatkem glukózy a kyselinou olejovou v lidských hepatomových HepG2 buňkách (cs)
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| skos:prefLabel
| - mRNA levels of peroxisome proliferator-activated receptors and their coactivators are affected by glucose deprivation and oleate in heuman hepatoma HepG2 cells
- mRNA levels of peroxisome proliferator-activated receptors and their coactivators are affected by glucose deprivation and oleate in heuman hepatoma HepG2 cells (en)
- Hladiny mRNA receptorů aktivovaných proliferátory peroxisomů a jejich koaktivátorů jsou ovlivněny nedostatkem glukózy a kyselinou olejovou v lidských hepatomových HepG2 buňkách (cs)
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| skos:notation
| - RIV/61989592:15110/07:00004428!RIV08-MSM-15110___
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| http://linked.open.../vavai/riv/strany
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
| - P(OC 153), Z(MSM6198959205)
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/61989592:15110/07:00004428
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - Starvation; Glucose; Oleic acid; Peroxisome proliferator-activated receptor (PPAR); PPAR-gama coactivator (PGC); mRNA stability; Superinduction; HepG2 cells (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...odStatuVydavatele
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
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| http://linked.open...in/vavai/riv/obor
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| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...vavai/riv/projekt
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| http://linked.open...UplatneniVysledku
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| http://linked.open...v/svazekPeriodika
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| http://linked.open...iv/tvurceVysledku
| - Veselý, Jaroslav
- Uherková, Lenka
- Rypka, Miroslav
- Bogdanová, Kateřina
- Poczatková, Hana
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| http://linked.open...n/vavai/riv/zamer
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| issn
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| number of pages
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| http://localhost/t...ganizacniJednotka
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