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| Description
| - We compared the effects of chelerythrine (CHE) and sanguinarine (SA) on human prostate cancer cell lines (LNCaP and DU-145) and primary culture of human gingival fibroblasts. CHE and SA treatment of cell lines for 24 h resulted in (1) inhibition of cell viability in a dose-dependent manner in all tested cells (as evaluated by MTT test and bromodeoxyuridine incorporation assay); (2) dose-dependent increase in DNA damage in all tested cells (as evaluated by DNA comet assay); (3) changes in apoptosis (assessed by western blot analysis and TUNEL assay); and (4) significant induction of cyclin kinase inhibitors p21(Waf1/Cip1) and p27(Kip1) in prostate cancer cells (identified by western blot analysis). Our study demonstrates that CHE had significant cytotoxic effect, independent of p53 and androgen status, on human prostate cancer cell lines. Normal gingival fibroblasts and DU-145 cells were more sensitive to the treatment with both alkaloids than were LNCaP cells. CHE and SA may be prospective natural mol
- We compared the effects of chelerythrine (CHE) and sanguinarine (SA) on human prostate cancer cell lines (LNCaP and DU-145) and primary culture of human gingival fibroblasts. CHE and SA treatment of cell lines for 24 h resulted in (1) inhibition of cell viability in a dose-dependent manner in all tested cells (as evaluated by MTT test and bromodeoxyuridine incorporation assay); (2) dose-dependent increase in DNA damage in all tested cells (as evaluated by DNA comet assay); (3) changes in apoptosis (assessed by western blot analysis and TUNEL assay); and (4) significant induction of cyclin kinase inhibitors p21(Waf1/Cip1) and p27(Kip1) in prostate cancer cells (identified by western blot analysis). Our study demonstrates that CHE had significant cytotoxic effect, independent of p53 and androgen status, on human prostate cancer cell lines. Normal gingival fibroblasts and DU-145 cells were more sensitive to the treatment with both alkaloids than were LNCaP cells. CHE and SA may be prospective natural mol (en)
- V této studii jsme porovnávali vliv chelerythrinu (CHE) a sanguinarinu (SA) na lidské prostatické nádorové buněčné linie (LNCaP a DU-145) a primární kulturu lidských gingiválních fibroblastů. Buňky byly vystaveny 24 h působení SA a CHE. Poté bylo pozorováno (1) snížení životnosti buněk v závislosti na koncentraci SA a CHE u všech testovaných buněčných modelů (hodnoceno MTT testem a inkorporací bromdeoxyuridinu do buněk); (2) nárůst poškození DNA v závislosti na koncentraci látek u všech testovaných buněk (hodnoceno metodou Comet essay); (3) změny v hladinách proteinů zapojených do procesu apoptózy (sledované western blot analýzou a metodou TUNEL); (4) signifikantní indukce inhibitorů cyklin-dependentních kinas p21Waf1/Cip1 a p27Kip1 u prostatických nádorových buněčných linií (stanovené western blot analýzou). V této studii jsme dokázaly, že CHE má statisticky významný cytotoxický účinek na prostatické nádorové buněčné linie, nezávislý na obsahu proteinu p53 a androgenu. Normální gingivální fibroblast (cs)
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| Title
| - The effect of chelerythrine on cell growth, apoptosis, and cell cycle in human normal and cancer cells in comparison with sanguinarine
- Porovnání vlivu chelerythrinu a sanguinarine na buněčný růst, apoptózu a buněčný cyklus u lidských gingiválních fibroblastů a nádorových buněk (cs)
- The effect of chelerythrine on cell growth, apoptosis, and cell cycle in human normal and cancer cells in comparison with sanguinarine (en)
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| skos:prefLabel
| - The effect of chelerythrine on cell growth, apoptosis, and cell cycle in human normal and cancer cells in comparison with sanguinarine
- Porovnání vlivu chelerythrinu a sanguinarine na buněčný růst, apoptózu a buněčný cyklus u lidských gingiválních fibroblastů a nádorových buněk (cs)
- The effect of chelerythrine on cell growth, apoptosis, and cell cycle in human normal and cancer cells in comparison with sanguinarine (en)
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| skos:notation
| - RIV/61989592:15110/06:00003481!RIV07-MSM-15110___
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| http://linked.open.../vavai/riv/strany
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/61989592:15110/06:00003481
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - Apoptosis; Cell cycle; Chelerythrine; Cytotoxicity; DNA damage; Sanguinarine (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...odStatuVydavatele
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
| - Cell Biology and Toxicology
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| http://linked.open...in/vavai/riv/obor
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| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...UplatneniVysledku
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| http://linked.open...v/svazekPeriodika
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| http://linked.open...iv/tvurceVysledku
| - Ulrichová, Jitka
- Malíková, Jana
- Hlobilková, Alice
- Zdařilová, Adéla
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| http://linked.open...n/vavai/riv/zamer
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| issn
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| number of pages
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| http://localhost/t...ganizacniJednotka
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