| Attributes | Values |
|---|
| rdf:type
| |
| Description
| - Cytochrom P450 (CYP) 1A1 přitahuje pozornost hlavně prosvou úlohu v tvorbě karcinogenních reaktivních metabolitů z polycyklických aromatických uhlovodíků jako je benzo-a-pyren, ale recentní poznatky naznačují jeho zapojení do regulace buněčného cyklu. Exprese tohoto enzymu je regulována receptorem pro aromatické uhlovodíky (AhR). Ukázali jsme, že indukce CYP1A1 dioxinem v primárních potkaních hepatocytech a HepG2 buňkách je narušena kolchicinem a nokodazolem. Obě sloučeniny snížily obsah CYP1A1 proteinu, mRNA a katalytickou aktivitu v HepG2 buňkách a primárních potkaních hepatocytech. Žádná ze sloučenin neovlivnila vazbu 3H-TCDD do AhR, tedy jejich účinek na transkripční aktivitu AhR je nepřímý. Jelikož kolchicin a nokodazol jsou látky narušující mikrotubuly, zkoumali jsme jejich účinek na integritu mikrotubulů v obou typech buněk.Obě sloučeniny rozbily mikrotubulární síť s odlišnou účinností, v závislosti na typu buněk. Pozorované potlačení transkripční aktivity AhR kolchicinem a nokodazolem by v Hep (cs)
- Cytochrome P450 (CYP) 1A1 attracts attention mainly because of its role in production of carcinogenic reactive metabolites from polycyclic aromatic hydrocarbons such as benzo[a]pyrene, but recent developments indicate its apparent role in cell cycle progression. Expression of the enzyme is subject to regulation by aryl hydrocarbon receptor (AhR). It has been shown that induction of CYP 1A1 in HepG2 cells and primary rat hepatocytes by tetrachloro-p-dibenzodioxin (TCDD) is diminished by colchicine and nocodazole. Both compounds decrease CYP1A1 mRNA, protein, and activity levels in HepG2 cells and mRNA level in primary rat hepatocytes. Neither compound significantly affected [(3)H]-TCDD binding to AhR, thus their effect on AhR transcriptional activity proceeds via indirect means. For colchicine and nocodazole are well-known microtubule interfering agents, we also assessed their effect on microtubule integrity in both cell types under investigation. Both compounds disrupt cytoskeleton integrity with diff
- Cytochrome P450 (CYP) 1A1 attracts attention mainly because of its role in production of carcinogenic reactive metabolites from polycyclic aromatic hydrocarbons such as benzo[a]pyrene, but recent developments indicate its apparent role in cell cycle progression. Expression of the enzyme is subject to regulation by aryl hydrocarbon receptor (AhR). It has been shown that induction of CYP 1A1 in HepG2 cells and primary rat hepatocytes by tetrachloro-p-dibenzodioxin (TCDD) is diminished by colchicine and nocodazole. Both compounds decrease CYP1A1 mRNA, protein, and activity levels in HepG2 cells and mRNA level in primary rat hepatocytes. Neither compound significantly affected [(3)H]-TCDD binding to AhR, thus their effect on AhR transcriptional activity proceeds via indirect means. For colchicine and nocodazole are well-known microtubule interfering agents, we also assessed their effect on microtubule integrity in both cell types under investigation. Both compounds disrupt cytoskeleton integrity with diff (en)
|
| Title
| - Involvement of cytoskeleton in AhR-dependent CYP1A1 expression
- Zapojení cytoskeletu do AhR-závislé exprese CYP1A1 (cs)
- Involvement of cytoskeleton in AhR-dependent CYP1A1 expression (en)
|
| skos:prefLabel
| - Involvement of cytoskeleton in AhR-dependent CYP1A1 expression
- Zapojení cytoskeletu do AhR-závislé exprese CYP1A1 (cs)
- Involvement of cytoskeleton in AhR-dependent CYP1A1 expression (en)
|
| skos:notation
| - RIV/61989592:15110/06:00003106!RIV07-GA0-15110___
|
| http://linked.open.../vavai/riv/strany
| |
| http://linked.open...avai/riv/aktivita
| |
| http://linked.open...avai/riv/aktivity
| - P(1P05ME767), P(GP303/04/P074), Z(MSM6198959216)
|
| http://linked.open...iv/cisloPeriodika
| |
| http://linked.open...vai/riv/dodaniDat
| |
| http://linked.open...aciTvurceVysledku
| |
| http://linked.open.../riv/druhVysledku
| |
| http://linked.open...iv/duvernostUdaju
| |
| http://linked.open...titaPredkladatele
| |
| http://linked.open...dnocenehoVysledku
| |
| http://linked.open...ai/riv/idVysledku
| - RIV/61989592:15110/06:00003106
|
| http://linked.open...riv/jazykVysledku
| |
| http://linked.open.../riv/klicovaSlova
| - AhR receptor; Cell Cycle; Cytochrome P450; Drug Metabolism; Microtubules (en)
|
| http://linked.open.../riv/klicoveSlovo
| |
| http://linked.open...odStatuVydavatele
| |
| http://linked.open...ontrolniKodProRIV
| |
| http://linked.open...i/riv/nazevZdroje
| |
| http://linked.open...in/vavai/riv/obor
| |
| http://linked.open...ichTvurcuVysledku
| |
| http://linked.open...cetTvurcuVysledku
| |
| http://linked.open...vavai/riv/projekt
| |
| http://linked.open...UplatneniVysledku
| |
| http://linked.open...v/svazekPeriodika
| |
| http://linked.open...iv/tvurceVysledku
| - Dvořák, Zdeněk
- Ulrichová, Jitka
- Vrzal, Radim
- Modrianský, Martin
- Maurel, Patrick
- Pascussi, Jean-Marc
|
| http://linked.open...n/vavai/riv/zamer
| |
| issn
| |
| number of pages
| |
| http://localhost/t...ganizacniJednotka
| |
![[RDF Data]](/fct/images/sw-rdf-blue.png)
![[cxml]](/fct/images/cxml_doc.png)
![[csv]](/fct/images/csv_doc.png)
![[text]](/fct/images/ntriples_doc.png)
![[turtle]](/fct/images/n3turtle_doc.png)
![[ld+json]](/fct/images/jsonld_doc.png)
![[rdf+json]](/fct/images/json_doc.png)
![[rdf+xml]](/fct/images/xml_doc.png)
![[atom+xml]](/fct/images/atom_doc.png)
![[html]](/fct/images/html_doc.png)