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| rdf:type
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| Description
| - Kryoprezervace poskytuje možnost maximalizovat využití a dostupnost biologických materiálů, jejichž zdroje jsou omezené. Tato studie předkládá zlepšenou techniku současné kryoprezervace serie řezů jaterní tkáně s využitím přípravku z hliníkové folie při nízké koncentraci kryoprotektantu. Rychlost zchlazení a zahřívání přibližně 2000 a 3900 °C.min-1 je dosažena významným snížením tepelné kapacity hliníkové folie ve srovnání se sandwichovým zařízením, popsaným Dayem et al. [S.H. Day, D.A. Nicoll-Griffith, J.M. Silva, Cryopreservation of rat and human liver slices by rapid freezing, Cryobiology 38 (1999) 154-159]. Viabilita řezů byla ověřována měřením redukce tetrazoliové soli, uvolňování cytosolové laktátdehydrogenázy do media a metabolismu ethoxykumarinu. (cs)
- Cryopreservation oVers the potential to maximize the use and availability of biological materials that have a limited supply. This study demonstrates an enhanced technique for the parallel cryopreservation of a series of liver tissue slices using a tray modeled from aluminium foil and low concentrations of a cryoprotectant. Cooling and warming rates of approximately 2000 and 3900 °Cminˇ1, respectively, were achieved as the thermal capacity of the foil-tray was significantly reduced compared to the aluminium sandwich device introduced by Day et al. [S.H. Day, D.A. Nicoll-Grifith, J.M. Silva, Cryopreservation of rat and human liver slices by rapid freezing, Cryobiology 38 (1999) 154-159]. Additionally, the two critical steps involved in the sandwich approach, i.e., clamping the plates and complete filling of the entire space between the plates with liquid, can be omitted using the foil tray. The viability of the slices was verified by measuring tetrazolium salt reduction capacity, cytosolic enzyme lact
- Cryopreservation oVers the potential to maximize the use and availability of biological materials that have a limited supply. This study demonstrates an enhanced technique for the parallel cryopreservation of a series of liver tissue slices using a tray modeled from aluminium foil and low concentrations of a cryoprotectant. Cooling and warming rates of approximately 2000 and 3900 °Cminˇ1, respectively, were achieved as the thermal capacity of the foil-tray was significantly reduced compared to the aluminium sandwich device introduced by Day et al. [S.H. Day, D.A. Nicoll-Grifith, J.M. Silva, Cryopreservation of rat and human liver slices by rapid freezing, Cryobiology 38 (1999) 154-159]. Additionally, the two critical steps involved in the sandwich approach, i.e., clamping the plates and complete filling of the entire space between the plates with liquid, can be omitted using the foil tray. The viability of the slices was verified by measuring tetrazolium salt reduction capacity, cytosolic enzyme lact (en)
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| Title
| - A novel simplified ultra-rapid freezing technique for cryopreservation of tissue slices
- A novel simplified ultra-rapid freezing technique for cryopreservation of tissue slices (en)
- Nová zjednodušená technika ultrarychlého zmrazování pro kryoprezervaci tkáňových řezů (cs)
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| skos:prefLabel
| - A novel simplified ultra-rapid freezing technique for cryopreservation of tissue slices
- A novel simplified ultra-rapid freezing technique for cryopreservation of tissue slices (en)
- Nová zjednodušená technika ultrarychlého zmrazování pro kryoprezervaci tkáňových řezů (cs)
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| skos:notation
| - RIV/61989592:15110/06:00002714!RIV07-MSM-15110___
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| http://linked.open.../vavai/riv/strany
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| http://linked.open...avai/riv/aktivita
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| http://linked.open...avai/riv/aktivity
| - P(OC B17.20), Z(MSM6198959205)
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| http://linked.open...iv/cisloPeriodika
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| http://linked.open...vai/riv/dodaniDat
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| http://linked.open...aciTvurceVysledku
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| http://linked.open.../riv/druhVysledku
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| http://linked.open...iv/duvernostUdaju
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| http://linked.open...titaPredkladatele
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| http://linked.open...dnocenehoVysledku
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| http://linked.open...ai/riv/idVysledku
| - RIV/61989592:15110/06:00002714
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| http://linked.open...riv/jazykVysledku
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| http://linked.open.../riv/klicovaSlova
| - Cryopreservation; Precision-cut slices; Ultra-rapid freezing; Warming rate; Mouse liver (en)
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| http://linked.open.../riv/klicoveSlovo
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| http://linked.open...odStatuVydavatele
| - GB - Spojené království Velké Británie a Severního Irska
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| http://linked.open...ontrolniKodProRIV
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| http://linked.open...i/riv/nazevZdroje
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| http://linked.open...in/vavai/riv/obor
| |
| http://linked.open...ichTvurcuVysledku
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| http://linked.open...cetTvurcuVysledku
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| http://linked.open...vavai/riv/projekt
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| http://linked.open...UplatneniVysledku
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| http://linked.open...v/svazekPeriodika
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| http://linked.open...iv/tvurceVysledku
| - Veselý, Jaroslav
- Uherková, Lenka
- Rypka, Miroslav
- Florschutz, Anthony V.
- Poczatková, Hana
- Červenková, Kateřina
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| http://linked.open...n/vavai/riv/zamer
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| issn
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| number of pages
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| http://localhost/t...ganizacniJednotka
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